Pei-Sze Ng
Purdue University
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Featured researches published by Pei-Sze Ng.
Bioconjugate Chemistry | 2010
Pei-Sze Ng; Brian M. Laing; Ganesan Balasundarum; Maneesh Pingle; Alan M. Friedman; Donald E. Bergstrom
A series of aliphatic and aromatic spacer molecules designed to cap the ends of DNA duplexes have been synthesized. The spacers were converted into dimethoxytrityl-protected phosphoramidites as synthons for oligonucleotides synthesis. The effect of the spacers on the stability of short DNA duplexes was assessed by melting temperature studies. End-caps containing amide groups were found to be less stabilizing than the hexaethylene glycol spacer. End-caps containing either a terthiophene or a naphthalene tetracarboxylic acid diimide were found to be significantly more stabilizing. The former showed a preference for stacking above an A*T base pair. Spacers containing only methylene (-CH(2)-) and amide (-CONH-) groups interact weakly with DNA and consequently may be optimal for applications that require minimal influence on DNA structure but require a way to hold the ends of double-stranded DNA together.
Nucleosides, Nucleotides & Nucleic Acids | 2003
Pei-Sze Ng; Maneesh Pingle; Ganesan Balasundarum; Alan M. Friedman; Xiaolin Zu; Donald E. Bergstrom
Abstract The syntheses of endcaps for covalently linking the 3′ and 5′ hydroxyl groups of blunt end double-stranded DNA are described. Endcap diols were converted into DMTr protected phosphoramidites and incorporated between nucleotides 4 and 5 of a self-complementary octamer. The stabilizing effect of the endcaps on duplex DNA was determined by Tm experiments on the self-complementary octamer.
Current protocols in human genetics | 2003
Maneesh Pingle; Pei-Sze Ng; Xiaolin Xu; Donald E. Bergstrom
Endcaps may be either aromatic or aliphatic molecules that specifically cross‐link the 5′ end of one strand with the 3′ end of the complementary strand in a DNA duplex. Endcaps may be viewed as a replacement of the loop region nucleotides of a DNA hairpin, with the added advantage of increased thermal stability. An endcap is incorporated into the sequence during oligonucleotide synthesis. Three endcaps are described in this unit. The naphthalene diimide endcap prefers to base stack with GC base pairs. The terthiophene endcap has higher lipophilicity than the naphthalene diimide endcap and provides higher stability when stacked over an AT base pair. The 2,2′‐oxydiacetamide endcap provides lower enhancement in stability but a more rigid and well‐defined structure than the oligo(ethylene glycol) endcaps. Synthesis of endcapped oligonucleotides can be carried out using standard automated synthesis protocols with only minor modifications.
Archive | 2011
Scott Eliasof; Oliver S. Fetzer; Jungyeon Hwang; Soo Patrick Lim; Pei-Sze Ng; Sonke Svenson; Donald E. Bergstrom
Nano Letters | 2005
Pei-Sze Ng; Donald E. Bergstrom
Nucleic Acids Research | 2004
Pei-Sze Ng; Donald E. Bergstrom
Archive | 2011
Oliver S. Fetzer; Jungyeon Hwang; Soo Patrick Lim; Pei-Sze Ng; Sonke Svenson
Archive | 2015
Scott Eliasof; Oliver S. Fetzer; Jungyeon Hwang; Patrick Lim Soo; Pei-Sze Ng; Sonke Svenson; Donald E. Bergstrom
Archive | 2014
エス. フェッツァー オリバー; S Fetzer Oliver; ホワン, ジュンヨン; Jungyeon Hwang; リム スー, パトリック; Lim Soo Patrick; ン, ペイ−シェ; Pei-Sze Ng; スフェンゾン, ゾンケ; Sonke Svenson
Archive | 2011
Donald E. Bergstrom; Scott Eliasof; Oliver S. Fetzer; Jungyeon Hwang; Soo Patrick Lim; Pei-Sze Ng; Sonke Svenson