Peigen Xiao

Chemical analysis and anti-inflammatory comparison of the cell culture of Glycyrrhiza with its field cultivated variety.

Suspended cells of Glycyrrhiza (CG) possessed a similar content of flavonoids and a lower content of triterpenes, when compared with its field cultivated equivalent (NG). Xylene-induced ear oedema and ovalbumin-induced mouse paw oedema were applied, to compare the effects of CG and NG on the acute inflammatory response. Extracts of the cell culture of Glycyrrhiza possessed a similar anti-inflammatory effect to those of NG, through the enhancement of the SOD activity of plasma and liver tissues. The use of a cell culture of liquorice instead of field cultivation would be potentially profitable.

Effect of bioreactor angle and aeration rate on growth and hydromechanics parameters in bioreactor culture of ginseng suspension cells

Suspension cells of Panax ginseng C.A. Meyer were cultivated in 3-L balloon-type bubble bioreactors and the bioreactor with the angle of 90° at the bottom side was optimized. The gaseous composition in plant cell and tissue cultures is regarded as an important factor affecting the plant growth. Gas hold-up was remarkably higher in the bioreactor with an angle of 90° than the other ones. Aeration rates impacted on the growth ratio, the specific O2 uptake rate (SOUR) of ginseng cells were investigated. 0.4xa0vvm was selected as the optimal aeration rate with a dry weight of 6.45xa0gxa0L−1. The specific O2 uptake rate in the culture time was detected and reached the top value at the maximum growth ratio.

Production of flavonoids and polysaccharide by adding elicitor in different cellular cultivation processes of Glycyrrhiza uralensis Fisch

In this study, callus and cell suspension were induced from seedlings of licorice (G. uralensis). In addition, it was revealed that the appropriate concentration of sucrose could promote the callus growth and increase the content of polysaccharide. The methyl jasmonate (MJ) and phenylalanine (PHE) could enhance the callus growth and content of flavonoids for G. uralensis. For producing more flavonoids and polysaccharide, two-stage cultivation was performed. In the first step, 30xa0gxa0L−1 sucrose was fed into a 5-L balloon-type bubble bioreactor on 8th day of culture to enhance cell production and metabolite production. In a two-stage cultivation process, PHE (2xa0mM) and MJ (5xa0mgxa0L−1) were added into a 5-L balloon-type bubble bioreactor after 10 days of culture. Using a fed-batch cultivation strategy (30xa0gxa0L−1 sucrose was fed into a 5-L balloon-type bubble bioreactor on 8th day), polysaccharide production was enhanced to 1.19xa0gxa0L−1, which was 2.12-fold greater than that in batch cultivation. The flavonoids yield (55.42xa0mgxa0L−1) which was about 22xa0% higher than that in batch cultivation was obtained on 21stxa0day. In a two-stage cultivation process, the polysaccharide content was increased by 1.14- and 2.12-fold compared with fed-batch cultivation and batch cultivation on 15th day. Meanwhile, total flavonoids yield (132.36xa0mgxa0L−1) on 15th day, was increased by 2.26- and 2.67-fold compared with fed-batch cultivation and batch cultivation. In conclusion, two-stage cultivation process combined with the sucrose and elicitor treatment could promote both the callus growth and the secondary metabolites accumulation.