Peng Qiang

Differential effects of various trans fatty acids isomers on apoptosis rate of human umbilical vein endothelial cell in vitro

Objective To explore the effects of trans fatty acids (TFA) isomers on apoptosis rate of human umbilical vein endothelial cell (HUVECs) in vitro. Method we obtained the original generation of HUVECs from umbilical cord. All cells were divided into six groups, including one control group and five TFA isomers groups, and cell count of every group was regulated under 0.5∼1×106. In TFA isomers groups, cells were cultured in a medium containing 200 μmol/ml different five TFA isomers, namely trans palmitic acid (Δ9 T-C16:1), trans linoleic acid (Δ9, Δ12 T-C18:2), Δ6 trans oleic acid (Δ6 T-C18:1), Δ9 trans oleic acid (Δ9 T-C18:1), Δ11 trans oleic acid (Δ11 T-C18:1). After 24 h of incubation, cells were detected the apoptosis rate by using cytometry techniques. Results Compared with the control group, the apoptosis rate of every TFA isomers groups were significantly higher. The apoptosis of T-C16:1, T-C18:2, Δ6 T-C18:1, Δ9 T-C18:1 and Δ11 T-C18:1 were, respectively 12.84±2.7%, 29.38±4.7%, 25.12±3.6%, 22.76±3.4% and 15.49±2.7%. In three different T-C18:1 isomers, the apoptosis of Δ6 T-C18:1 and Δ9 T-C18:1 groups were higher than Δ11 T-C18:1 group. Conclusion Under the same TFA isomers concentrations, there are differences between apoptosis rate of HUVECs in vitro.

ASSA13-14-24 The Seasonal Variation of Blood Pressure in Hypertensive Patients Treated with Combination Therapy in CHIEF Trial

Objective To investigate the seasonal variation of BP level and BP control rate in patients with well controlled BP by combination therapy. Methods A total of 332 hypertensive patients enrolled in Chinese Hypertension Intervention Efficacy Study (CHIEF) in Nanchang area. The patients were enrolled from February to August of 2008 and randomised to amlodipine + compound amiloride (group AA) and amlodipine + telmisartan (group AT) therapy. Their BP was reached to target BP by gradually increasing the dosage of drugs at the 12 week. The 2 years data up to June 2010 were collected and the BP on follow-up week was translated to on calendar month. The average month temperature was from Jiangxi Provincial Meteorological Observatory. Results 1. The BP curve on week decreased gradually as the following duration increased, but the BP curve on calendar month showed obvious seasonal variation, with the BP peak in cold season during the 2 years period. The SBP increment amplitude in cold season was about 9.7–10.5 mmHg and DBP about 3.1–3.9 mmHg. 2. Generally, BP control rates on week increased from 70% to 90% as following duration increased. But the BP control rates on calendar month varied significantly from 58.1% to 95.5%, with dips in the cold seasons. 3. No significant deference on BP level, BP control rate and BP seasonal variation was seen between AA and AT groups. Conclusions Even in BP well controlled patients with combination therapy exits obvious seasonal variation of BP control rate.

ASSA13-16-6 The Increased Circulatory RAS Activity Can Be Inhibited by Statins

Objective To investigate the profile of circulatory renin-angiotensin system (RAS) activity in hypercholesterolemia (HC) patients treated with statins. Methods This study included 18 primary HC patients and 18 sex-and age-matched healthy adults. Total cholesterol (TC), triglyceride (TG), LDL-C and blood glucose, angiotensin-converting enzyme (ACE) activity and angiotensin II (AngII) levels were measured at baseline. These parameters were measured again at 4 and 8 weeks after statin treatment in HC group respectively. Results At baseline, the TC, TG and LDL-C, as well as ACE activity and AngII level, were significantly higher in HC group. On the baseline data of 36 participates, significant positive correlations existed between ACE activity and TC(r = 0.54) or LDL-C(r = 0.51), and between AngII level and TC(r = 0.34) or LDL-C(r = 0.27). In HC patients, 8 weeks statin treatment significantly decreased TC, LDL-C, as well as Ang II (35.46 ± 14.49 vs 71.10 ± 20.47 pg/ml, P < 0.05) levels and ACE activity (108.9 ± 51.9vs 180.1 ± 71.3 U/L, P < 0.05), meanwhile, positive correlations between RAS activity and TC or LDL-C levels measured before and after treatment were seen in HC patients. Conclusions Serum cholesterol-lowering with statins is associated with decreasing circulatory RAS activity in hypercholesterolemia patients.

ASSA13-16-4 The Influence of Combination of Amlodipine and Valsartan on Plasma Free Fat Acid and Adiponectin in Patients with Essential Hypertension

Objective To investigate the influence of combination therapy of amlodipine and valsartanon on the plasma adiponectin (ADPN) and free fatty acids (FFAs) levels in patients with essential hypertension. Methods Fifty hypertensive patients were included. After 4 week amlodipine (5mg/d) treatment, 34 patients with diastolic blood pressure (DBP) equal to or more than 90 mmHg were included in the double blind period and randomly divided into amlodipine (amlodipine, 5mg/d) and the combination (amlodipine 5mg + valsartan 80 mg/d) groups for other 8 weeks. BP, blood lipids, blood glucose, ADPN and FFA were measured at baseline, 4 and 12 week after treatment. Eighteen normotensive adults were selected as the control. Results At baseline, FFA, total cholesterol (TC) and triglycerides (TG) were significantly higher, but ADPN lower in the hypertensive group than in the control group. Four weeks amlodipine treatment increased significantly ADPN levels (4.75 ± 1.88 vs 5.09 ± 1.97 mg/L, P. Conclusions Four weeks amlodipine monotherapy can significantly increase the plasma ADPN level. On this base, the combination therapy of amlodipine and valsartan can better improve plasma ADPN and FFA than amlodipine monotherapy.

ASSA13-14-27 The Impact of Blood Pressure Levels, Gender and Age on Within-Visit Systolic Blood Pressure Variability

Objective The study aims to investigate the variability in three blood pressure (BP) readings within one measurement (within-visit BPV) and the relevant influencing factors. Methods Three BP readings were taken at 2-min interval in 2092 adults (1028 males and 1064 females) with mercurial sphygmomanometer. The average of three BP readings was used as the mean BP. The standard deviation (SD) of three BP readings and coefficient of variation (CV: SD/mean BP) were calculated for evaluating within-visit BPV. According to the history of hypertension and use of antihypertensive drugs, the participants were divided into hypertensive (EH group, 756 cases) and normotensive groups (NT group,). Multiple stepwise regression analysis was used to analyse the relevant influencing factors. Results 1. A positive relationship existed between the mean SBP rank and SD(r = 0.168, P < 0.01) in the whole population. Although the SBP and SD were higher in EH group than in NT group, no significant difference of CV was found between two groups. 2. The SD and CV were significantly higher in females than in males although lower SBP in female. 3 Positive relationship was found between age rank and SD (r = 0.129, P < 0.01) and between age rank and CV (r = 0.153, P < 0.01), with stronger relationship in females (r = 0.137 and 0.166, both P < 0.05) than in males (r = 0.098 and 0.122, both P < 0.05). Conclusions The mean SBP, female and ageing are related with higher within-visit SBP variability, but female and ageing are independent facilitating factors.

ASSA13-14-26 Preoperative Anxiety is Associated with Higher Blood Pressure in Patients Waiting For Surgery

Objective The relationship of preoperative anxiety and blood pressure (BP) has not been fully investigated. This study assesses the impact of preoperative anxiety on BP. Methods A total of 660 in-patients waiting for elective surgery were enrolled. Repeated BPs were measured after admission (basic BP), in every morning, after anesthesiologist visit (BPv) and 30 min before entering operation room (BPe). The BPs taken in the 3 days before and after operation were recorded as BP-3, BP-2 and BP-1 as well as BP1, BP2 and BP3 respectively. The difference between the BPe and basic BP was calculated as the BP increase amplitude (ΔBP). The anxiety scale was evaluated ( > 50 points anxiety) after anesthesiologist visit for all patients. Results The SBP and DBP continuously increased before operation and to the peak before entering operation room. The SBPe (136 ± 15 vs 118 ± 14mmHg, P < 0.05) and the DBPe (79 ± 9 vs 72 ± 9mmHg, P < 0.05) were significantly higher than their basic values respectively. After operation both SBP and DBP decreased. The prevalence of preoperative anxiety was 37.1%. The anxiety group has higher basic SBP, pre-operative SBP and ΔSBP, but more rapid SBP and ΔSBP decline than non-anxiety group. The profile of DBP change was similar to, but weaker than that of SBP in the observation duration. Multifactor analysis demonstrated that anxiety scale was positively associated with ΔSBP. Conclusions The patients with anxiety have obvious BP fluctuation and anxiety scale is positively associated with the SBP increase during the preoperative duration.

ASSA13-16-5 The Difference of Postprandial Plasma Triglyceride, Adiponectin and C-Reactive Protein Between Young and Middle Age Men

Objective The profile of postprandial plasma adiponectin and high-sensitivity C-reactive protein (hsCRP) is unclear in young persons and middle-aged men. Methods This study included 11 young men (18.9 ± 1.7 year) and 11 (40.8 ± 3.8year) body mass indexes (BMI) matched middle –aged men (22.8 ± 4.58 vs 22.7 ± 4.40, NS). The plasma triglyceride (TG) levels were measured before and at 2, 4 and 6 hours after a fat meal, and adiponectin and hsCRP were measured before and 2 hour after fat meal. Results The fasting TG level was higher in middle-age group than in young group. The postprandial TG levels at 2, 4 and 6h decreased in the young group, but increased in middle age men. The TG-AUC was significantly higher in middle age men than in young men (2.41 ± 1.31 vs –0.84 ± 1.19, h.mmol/L, P < 0.05). The fasting adiponectin and hsCRP levels were similar between two groups. Both groups showed significantly increased postprandial adiponectin and hsCRP levels with similar increase amplitudes, so the postprandial adiponectin and hsCRP levels were also similar. Conclusions The postprandial TG levels after a fat meal are associated with age. Although middle-aged men have significant higher postprandial TG levels, the 2 h postprandial adiponectin and hsCRP levels are similar to those of the young men.

ASSA13-15-22 The Inter-Arm Blood Pressure Difference Induced by Transradial Coronary Intervention

Background and Objective Inter-arm blood pressure difference (IAD) indicates artery disease of arm. This study was to investigate whether the transradial coronary intervention (TRI) increases the prevalence of IAD. Methods This study enrolled 154 adult in-patients underwent the first selective TRI, and without radial artery occlusion (RAO) by palpation method after TRI. Bilateral brachial and radial artery BPs were simultaneously measured using two automatic BP measurement devices in the day before (BP0) and one day (BP1d), 7 day (BP7d), 3 month (BP3m) and 6 month (BP6m) after TRI. The difference of the left and right BPs was calculated as the ΔBP l-r and the absolute value of ΔBP l-r equal to or over 10mmHg was recognised as IAD. Results The bilateral brachial and radial SBP0 and DBP0 were similar. The brachial BP1d and BP7d of the catheterized right arm decreased significantly and were significantly lower than those of left arm. At the same time, the rates of sIAD on brachial artery were higher than the baseline (27.3% and 13.0% vs 7.1%, both P < 0.05). Even the brachial artery BP6m was similar to BP0, but sIAD on brachial artery was higher somewhat than baseline (9.1%). Similar results were seen in the bilateral radial artery BP and IAD also. Conclusions TRI can induce BP decrease in the catheterized arm and increase the prevalence of systolic IAD for at least 7 days in both brachial or radial artery sites.

ASSA13-14-10 The Heterogeneity of the Two Orthostatic Hypertension Populations Defined by Amplitude and Absolute Methods

Background Two methods have been used for diagnosing orthostatic hypertension (OHT), but it is unclear whether the populations with OHT defined by these 2 methods have similar demographic characteristics. Methods Supine and upright BPs were measured (Omron HEM-7101) in 916 adults with normal supine blood pressure (BP). The difference between upright and supine BP was recorded as ΔBP. The criteria for OHT on SBP (s-OHT) in amplitude method was ΔSBP ≥ 10mmHg and than in absolute value method was upright SBP≥140mmHg. The demographic characteristics and BP of 2 s-OHT groups were compared. Results In this population, the detection rate of s-OHT on amplitude method was significantly higher, than that on absolute method (21.8% vs 14.7%, P < 0.05). Only 91 the 135 s-OHT cases on absolute method were included into the 200 cases on amplitude method and the overlapping rate was 37.3%. The s-OHT group on absolute method shows higher male percentage, age, BMI, supine and upright SBP compared with amplitude method. Conclusions The detection rates of s-OHT vary with the defining methods in a community population. The two s-OHT groups defined by amplitude method and absolute method have obvious heterogeneity in demographic characteristics, supine and upright SBP, especially in young population.

The basal and postexercise blood lipid in young students with and without regular exercise habit

Objective To explore the effect of exercise habit on the basal and postexercise blood lipid in young students. Methods This study included 76 students (19.3±1.1 years) with and without good exercise habit. The total cholesterol TC), triacylglycerol (TG), low-density lipoprotein (LDL-C) and high-density lipoprotein (HDL-C) were measured before and after exercise of 900 m walking within 9 min. Results (1) The TG and LDL-C levels reduced significantly after exercise, but other parameters did not significantly change. (2). The two groups with and without regular exercise habit had similar sex ratio, age and BMI, but the basic levels of TC and LDL-C, and LDL/HDL ratio were significantly lower in the regular exercise group than in seldom exercise group. (3). In the regular exercise group the post exercise TG level was significantly lower than pre-exercise (0.69±0.39 vs 0.84±0.40 mmol/l, p<0.05), but did not in seldom exercise group (0.97±0.43 vs 1.01±0.40 mmol/l, NS). The decrease amplitude of TG after exercise was significantly greater in regular exercise group than in seldom exercise group (0.15±0.35 vs 0.04±0.26). Conclusion The young people with good exercise habits, not only have better fasting lipid profile, but can better use triglycerides in the exercise.