Per F. Marton

Low-grade intestinal lymphoma of intraepithelial T lymphocyties with concomitant enteropathy-associated T cell lymphoma: Case report suggesting a possible histogenetic relationship☆

In a low-grade small cell intestinal lymphoma found in a 41-year-old woman with celiac disease, the neoplastic intraepithelial lymphocytes (IELs) showed cytoplasmic granules, expressed the alpha/beta T cell antigen receptor (TCR), and stained positively for the IEL antibody, HML-1. The tumor cells carried the CD3 and CD7 T cell antigens, but were double negative for CD4 and CD8 and also lacked other T cell antigens (CD1, CD2, and CD5). Tumor cell monoclonality was proven by the demonstration of a rearranged TCR-beta chain gene. The special marker profile of the lymphoma may be explained by partial antigenic deletion of the phenotype which characterizes the majority of normal IELs (TCR alpha/beta+, CD3+, CD8+, or CD4+). Alternately, the tumorous cell clone might originate from an as yet unrecognized TCR alpha/beta+, CD3+, CD7+, CD4-, CD8- normal subset of IEL. In the presented case, a concomitant high-grade large cell lymphoma supports the assumption that enteropathy-associated T cell lymphomas (EATCLs) derive from the intestinal IEL population and suggests that EATCLs may be preceded by a low-grade IEL lymphoma.

Sarcoid reaction of hilar and paratracheal lymph nodes in patients treated for testicular cancer

Bilateral hilar lymph node enlargement developed in four patients 1 to 8 years after successful radiotherapy for testicular cancer Stage I was performed. Two had additional paratracheal involvement. Biopsy showed sarcoid reaction in all four cases. An increased frequency of sarcoid reaction is assumed in patients treated for testicular cancer. The pathogenesis remains unknown. The clinical and radiologic findings should not be misinterpreted as showing recurrent malignant disease. In an otherwise disease‐free patient in whom mediastinal lymph node enlargement develops, a biopsy should always be performed.

Nuclear feulgen DNA content and nuclear size in human breast carcinoma.

Biopsy specimens from 29 patients with operable breast carcinomas were examined by Feulgen-DNA microspectrophotometry. A diploid DNA stemline was found in ten tumors, seven of which were stage I carcinomas. In 19 tumors, ten of which were stage II cancers, a non-diploid DNA stemline was observed. The diploid tumors were most often estrogen receptor-positive (nine of nine examined carcinomas), whereas 13 of 19 non-diploid tumors studied did not contain estrogen receptors. The mean nuclear size of the non-diploid tumor cells was increased compared with that of the diploid malignant cells. Three grade I carcinomas were diploid, whereas three grade III tumors were non-diploid. Of the 23 grade II carcinomas, seven were diploid and 16 non-diploid. Hum Pathol 13:626-630, 1982.

Granulocytic sarcoma (chloroma) of the uterine cervix: report of two cases

Two cases of granulocytic sarcoma in the cervix are described, adding to the six previously reported cases. Both our cases were primarily misdiagnosed as other types of small-cell malignant tumors. Misdiagnosis often occurs in granulocytic sarcomas, especially for those in extraskeletal sites or when the tumor precedes development of frank leukemia. A conclusive diagnosis of granulocytic sarcoma depends upon the demonstration of granulocytic differentiation of the tumor cells. For this purpose, special staining methods like the Leder stain and the antilysozyme immunoperoxidase stain are particularly useful.

Induction of Maturation in Different Types of B‐Cell Lymphomas in Vitro with TPA and Antibodies to Surface Immunoglobulin

Cells from eight selected cases of human non‐Hodgkin lymphomas of various histological types (lymphocytic, centrocytic, centrocytic/centroblastic, and immunocytomas) were stimulated in vitro with 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (TPA) and anti‐immunoglobulins (anti‐Ig) against the surface immunoglobulin (sIg) on the tumour cells. Six of these cases responded by intracellular Ig accumulation as measured by flow cytofluorometry and direct phenotypical change into immunoblasts/plasmablasts as detected by light microscopic immunocytochemistry. However, the response to TPA alone varied considerably from ease to case. These findings suggest that many, if not all, B‐cell subsets have the capacity to develop directly into Ig‐synthesizing cells (immunoblasts and plasmablasts). However, conditions for eliciting such events may vary, depending on the phenotypical properties and differentiation stage.