Per Munk Nielsen

Application of tailormade pectinases

Abstract Heterologous expression of pectinolytic enzymes has made it possible to tailormake pectinolytic enzyme preparations. Composition of pectinase products for existing applications can be improved, and products for new applications can be made. Apple mash treatment with galactanase, rhamnogalacturonase and rhamnogalacturonan esterase improves the cloud stability of the juice. Pectin methyl esterase is useful to gelate fruits, and the gelling effect can be enhanced by a pectin hairy region degrading enzyme combination. Rhamno-galacturonase releases soluble high molecular rhamnogalacturonans from the soy cell wall, and the use of this enzyme to produce a fibre enriched soy protein product is demonstrated. Carrot mash treatment with combinations of polygalacturonase, pectin lyase, and rhamnogalacturonase gives a puree.