Pere Huguet
Autonomous University of Barcelona
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Publication
Featured researches published by Pere Huguet.
Journal of Cellular and Molecular Medicine | 2009
Jordi Guillen-Marti; Ramon Diaz; Maria T. Quiles; Manuel López-Cano; Ramon Vilallonga; Pere Huguet; Santiago Ramon-y-Cajal; Albert Sanchez-Niubo; Jaume Reventós; Manel Armengol; M.A. Arbós
Background: Incisional hernia is a common and important complication of laparotomies. Epidemiological studies allude to an underlying biological cause, at least in a subset of population. Interest has mainly focused on abnormal collagen metabolism. However, the role played by other determinants of extracellular matrix (ECM) composition is unknown. To date, there are few laboratory studies investigating the importance of biological factors contributing to incisional hernia development. We performed a descriptive tissue‐based analysis to elucidate the possible relevance of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in association with local cytokine induction in human incisional hernia tissues. The expression profiles of MMPs, TIMPs and pro‐inflammatory cytokine signalling were investigated in aponeurosis and skeletal muscle specimens taken intraoperatively from incisional hernia (n= 10) and control (n= 10) patients. Semiquantitative RT‐PCR, zymography and immunoblotting analyses were done. Incisional hernia samples displayed alterations in the microstructure and loss of ECM, as assessed by histological analyses. Moreover, incisional hernia tissues showed increased MMP/TIMP ratios and de‐regulated inflammatory signalling (tumor necrosis factor [TNFA] and interleukin [IL]‐6 tended to increase, whereas aponeurosis TNFA receptors decreased). The changes were tissue‐specific and were detectable at the mRNA and/or protein level. Statistical analyses showed several associations between individual MMPs, TIMPs, interstitial collagens and inflammatory markers. The increment of MMPs in the absence of a counterbalance by TIMPs, together with an ongoing de‐regulated inflammatory signalling, may contribute in inducing a functional defect of the ECM network by post‐translational mechanisms, which may trigger abdominal wall tissue loss and eventual rupture. The notable TIMP3 protein down‐regulation in incisional hernia fascia may be of pathophysiological significance. We conclude that this study may help to pinpoint novel hypotheses of pathogenesis that can lead to a better understanding of the disease and ultimately to improvement in current therapeutic approaches.
American Journal of Pathology | 2011
Ramon Diaz; Maria T. Quiles; Jordi Guillem-Marti; Manuel López-Cano; Pere Huguet; Santiago Ramon-y-Cajal; Jaume Reventós; Manel Armengol; M.A. Arbós
Incisional hernia often occurs following laparotomy and can be a source of serious problems. Although there is evidence that a biological cause may underlie its development, the mechanistic link between the local tissue microenvironment and tissue rupture is lacking. In this study, we used matched tissue-based and in vitro primary cell culture systems to examine the possible involvement of fascia fibroblasts in incisional hernia pathogenesis. Fascia biopsies were collected at surgery from incisional hernia patients and non-incisional hernia controls. Tissue samples were analyzed by histology and immunoblotting methods. Fascia primary fibroblast cultures were assessed at morphological, ultrastructural, and functional levels. We document tissue and fibroblast loss coupled to caspase-3 activation and induction of apoptosis-like cell-death mechanisms in incisional hernia fascia. Alterations in cytoskeleton organization and solubility were also observed. Incisional hernia fibroblasts showed a consistent phenotype throughout early passages in vitro, which was characterized by significantly enhanced cell proliferation and migration, reduced adhesion, and altered cytoskeleton properties, as compared to non-incisional hernia fibroblasts. Moreover, incisional hernia fibroblasts displayed morphological and ultrastructural alterations compatible with autophagic processes or lysosomal dysfunction, together with enhanced sensitivity to proapoptotic challenges. Overall, these data suggest an ongoing complex interplay of cell death induction, aberrant fibroblast function, and tissue loss in incisional hernia fascia, which may significantly contribute to altered matrix maintenance and tissue rupture in vivo.
Revista Española de Patología | 2009
Pere Huguet; Carme Dinarès; Carmela Iglesias; Carmen Mª. Blázquez; Felicia Marginean; Daniel Badia; Josep Castellví; Natalia Tallada; Santiago Ramón y Cajal
Resumen Para un patologo preparado, reconocer un tumor cuando presenta la morfologia caracteristica y esta en su localizacion habitual es facil. Pero cuando se presenta en un lugar insolito y ademas la biopsia para diagnostico es pequena, es facil caer en un error de orientacion y, aunque a veces las tecnicas auxiliares nos puedan ayudar, en algunas ocasiones no se concreta el diagnostico hasta que una biopsia completa o la extirpacion del tumor nos permiten ver la totalidad de la lesion, con la sobrecarga de tiempo, riesgo para el paciente y gasto economico que ello conlleva. El Prof. Rosai describe esta situacion con la historia que contaba Lauren V. Ackerman y que titulo «El hombre de Estambul»: el empecinamiento en no reconocer un tumor porque no esta en «su» lugar. Aportamos dos casos de sarcomas de partes blandas que se presentaron en localizaciones que podriamos llamar «invertidas»: un sarcoma sinovial mandibular y un mioepitelioma maligno yuxtaarticular en un dedo del pie y exponemos las dificultades que presentaron para su diagnostico inicial.
International Journal of Colorectal Disease | 2008
Javier de la Torre Fernández de Vega; Pere Huguet; Alfredo Santana; Carmen Ruiz de Miguel; Federico Rojo; Santiago Ramón y Cajal; Eduardo Salido
Sweat gland carcinoma is a rare and heterogeneous tumor. These tumors present a challenge for histologic interpretation for many reasons: they have a broad range of microscopic features, a lack of good correlation between histologic classification and biological behaviour, confusing terminology and similarity to metastatic carcinomas. The tumor is most frequently encountered in the axillae where apocrine glands are abundant. A few cases were also reported in other areas, which typically have less apocrine glands. Most apocrine carcinomas have arisen in adults older than 40 years of age, but occasional examples have been reported in young adult and adolescents. We report a pathologic, immunohistochemical and molecular investigation of an apocrine carcinoma in the perianal region. We also discuss the differential diagnosis of other sweat gland lesions with histochemical and immunohistochemical studies, and review the literature on this unusual neoplasm. The patient was a 63-year-old white man who presented a nodular lesion with 2 years duration in the perianal region. The tumor began to enlarge 4 months before the examination. Physical examination revealed a 28×13 mm, well-defined, subcutaneous nodule, which was firm and reddish and was attached to the overlying skin but had good mobility. The clinical diagnosis was angiofibroma and an excisional biopsy was performed. Macroscopically, a 35× 10 mm portion of perianal skin and underlying soft tissue that included a pedunculated lesion of 25×10 mm was received. No evidence of recurrent or metastatic disease was seen after a follow-up period of 36 months. The specimen was processed according to the usual histopathologic techniques and stained with hematoxylin– eosin, periodic acid Schiff’s before and after digestion with diastase, Alcian blue and Perls’ Prussian blue stain.
Medicina Oral Patologia Oral Y Cirugia Bucal | 2004
Pere Huguet; Javier de la Torre; Judith Pallarès; Marta Carrera; Francesc Soler; Blanca Espinet; Daniel Malet
Journal of Surgical Research | 2013
Manuel López-Cano; Manel Armengol; Maria T. Quiles; Alex Biel; Janice Velasco; Pere Huguet; Aleix Mestre; Lluís María Delgado; Francesc Xavier Gil; M.A. Arbós
Journal of The American Academy of Dermatology | 2007
Domingo Bodet; Laura Rodríguez-Cano; Ramon Bartralot; Jordi Mollet; Antonio Medina; Cristina de las Heras; Patricia Bassas; Pere Huguet; Vicente García-Patos
Pediatric Dermatology | 2008
Maureen Rogers; Domingo Bodet; Patricia Bassas; Ramon Bartralot; Jordi Mollet; Gloria Aparicio; Cristina de las Heras; Pere Huguet; Moisés Labrador; Vicente García-Patos
Medicina oral | 2001
Pere Huguet; Josep Castellví; Marta Ávila; María Alejo; Fina Autonell; Carles Basas; María Socorro Bescos
Revista Española de Patología | 2010
Pere Huguet; María Alejandra Gabaldón; Irene Sansano; María Eugenia Semidey; Natalia Tallada; Josep Castellví; Javier Hernández-Losa; Santiago Ramón y Cajal