Peter G. Condliffe

Iodine and the brain

Though the means of preventing iodine-deficiency disorders is obvious, the mechanisms are of great interest to developmental neurologists and psychologists. In particular, endemic cretinism is virtually unseen and so unstudied in the West, but the considerable research done by the Chinese is now bei

Two-stage gel electrophoresis: application to the separation of bovine thyroid stimulating hormone sub-units and calf thymus histones.

Abstract Sodium dodecylsulfate (SDS) can be removed from protein by gel electrophoresis. This principle is useful for separating protein bands which are close to each other in SDS gel electrophoresis. We accomplished this by “two-stage” gel electrophoresis. In this system, SDS gel electrophoresis was carried out as the first step. Gel electrophoresis was then continued (after replacing the buffer) without SDS. SDS was then eluted from the gel into the lower buffer during the second stage. Separation of the subunits was significantly improved relative to simple SDS gel electrophoresis.