Pieter Jan Arnoldus Maria Plomp

Use of a yeast strain expressing glucoamylase in malt whisky fermentations

Fig. 2. Simultaneous measurements of (left) H+-extrusion from and (right) of K + uptake into S. pombe cells using pH-electrode (Beckman) and K+-sensitive electrode (Orion), respectively; sidedness of the inhibitory effects of vanadate and dicyclohexylcarbodiimide (DCCD); C control, / vanadate, 2 electroporated + vanadate, 3 DCCD0 4 electroporated + DCCD. The electrodes were connected to two Beckman Selection 2000 pH meters. 1.25 % (fresh mass per volume) aqueous cell suspension in 1 mmol/L MES-triethanolamine buffer (pH o = 5). Ion transport was started by the addition of 20 mmol/L glucose. The concentration of inhibitors in the medium as well as during electroporation was 5 mmol/L.