Poliana Camila Marinello
Universidade Estadual de Londrina
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Publication
Featured researches published by Poliana Camila Marinello.
Tumor Biology | 2017
Larissa Sanches; Poliana Camila Marinello; Carolina Panis; Tatiane Renata Fagundes; José Andrés Morgado-Díaz; Julio Cesar Madureira de-Freitas-Junior; Rubens Cecchini; Alessandra Lourenço Cecchini; Rodrigo Cabral Luiz
Citral is a natural compound that has shown cytotoxic and antiproliferative effects on breast and hematopoietic cancer cells; however, there are few studies on melanoma cells. Oxidative stress is known to be involved in all stages of melanoma development and is able to modulate intracellular pathways related to cellular proliferation and death. In this study, we hypothesize that citral exerts its cytotoxic effect on melanoma cells by the modulation of cellular oxidative status and/or intracellular signaling. To test this hypothesis, we investigated the antiproliferative and cytotoxic effects of citral on B16F10 murine melanoma cells evaluating its effects on cellular oxidative stress, DNA damage, cell death, and important signaling pathways, as these pathways, namely, extracellular signal-regulated kinases 1/2 (ERK1/2), AKT, and phosphatidylinositol-3 kinase, are involved in cell proliferation and differentiation. The p53 and nuclear factor kappa B were also investigated due to their ability to respond to intracellular stress. We observed that citral exerted antiproliferative and cytotoxic effects in B16F10; induced oxidative stress, DNA lesions, and p53 nuclear translocation; and reduced nitric oxide levels and nuclear factor kappa B, ERK1/2, and AKT. To investigate citral specificity, we used non-neoplastic human and murine cells, HaCaT (human skin keratinocytes) and NIH-3T3 cells (murine fibroblasts), and observed that although citral effects were not specific for cancer cells, non-neoplastic cells were more resistant to citral than B16F10. These findings highlight the potential clinical utility of citral in melanoma, with a mechanism of action involving the oxidative stress generation, nitric oxide depletion, and interference in signaling pathways related to cell proliferation.
Life Sciences | 2016
Fernando Tadeu Trevisan Frajacomo; Camila S. Padilha; Poliana Camila Marinello; Flávia Alessandra Guarnier; Rubens Cecchini; José Alberto Duarte; Rafael Deminice
AIMS Well-characterized animal tumor models of cancer cachexia are warranted to elucidate underlying mechanisms and provide a better approach to the human scenario. We aimed to investigate whether solid Ehrlich carcinoma reproduces clinical, functional and biological conditions of tumor-induced cachexia in mice. METHODS Eight-week old female Swiss mice were subcutaneously inoculated with Ehrlich tumor cells (tumor-bearing, TB group) or vehicle (sham) into the right flank and monitored for 28days. Tumor histopathological features and tumor-host interaction, including tissue weight, muscle structure, strength and biochemical parameters were carried out. KEY FINDINGS Tumor growth curve demonstrated a linear pattern with no difference in final carcass weight between groups. A well-defined capsule composed by connective tissue infiltrated by inflammatory and neoplastic cells surrounded the tumors. The TB group had reduced handgrip strength, aside from lower cross sectional area (CSA) and critically reduced parametrial fat pads. Plasma parameters of lactate dehydrogenase (LDH), creatine kinase (CK) and tumor necrosis factor-α (TNF-α) were higher in the TB group, suggesting predominance of catabolic and pro-inflammatory activities. Conversely, food intake and tissue weight did not differ between groups. SIGNIFICANCE Our data elucidated that the solid Ehrlich tumor model is feasible and effective in reproducing some of the relevant issues experienced by cancer patients with cachexia. The solid Ehrlich carcinoma emerges as an alternative tool against more aggressive cancer cachexia models during preclinical research.
Muscle & Nerve | 2017
Poliana Camila Marinello; Sara Santos Bernardes; Flávia Alessandra Guarnier; Thamara Nishida Xavier da Silva; Fernando H. Borges; Natália M.D. Lopes; Andréa Name Colado Simão; André Armani; Rubens Cecchini; Alessandra Lourenço Cecchini
We sought to verify whether isoflavin‐beta (Iso‐β), a mixture of isoflavones with antioxidant properties, could prevent thyrotoxicosis‐induced loss of muscle mass and the participation of oxidative stress (OS) in the mechanisms of this prevention.
Frontiers in Physiology | 2018
Breno Quintella Farah; Sérgio Lc Rodrigues; Gustavo O. Silva; Rodrigo P. Pedrosa; Marilia A. Correia; Mauro Virgílio Gomes de Barros; Rafael Deminice; Poliana Camila Marinello; Neil A. Smart; Lauro C. Vianna; Raphael Mendes Ritti-Dias
Meta-analyses have shown that supervised isometric handgrip training reduces blood pressure in hypertensives. However, the mechanism(s) underlying these effects in medicated hypertensive patients, as well as the effects from home-based exercise training, is uncertain. The purpose of this study was to compare the effects of supervised and home-based isometric handgrip training on cardiovascular parameters in medicated hypertensives. In this randomized controlled trial, 72 hypertensive individuals (38–79 years old, 70% female) were randomly assigned to three groups: home-based, supervised isometric handgrip training or control groups. Home-based and supervised isometric handgrip training was completed thrice weekly (4 × 2 min at 30% of maximal voluntary contraction, with 1-min rest between bouts, alternating the hands). Before and after 12 weeks brachial, central and ambulatory blood pressures (BP), arterial stiffness, heart rate variability, vascular function, oxidative stress and inflammation markers were obtained. No significant (p > 0.05) effect was observed for ambulatory BP, arterial stiffness, heart rate variability, vascular function and oxidative stress and inflammatory markers in all three groups. Brachial BP decreased in the supervised group (Systolic: 132 ± 4 vs. 120 ± 3 mmHg; Diastolic: 71 ± 2 vs. 66 ± 2 mmHg, p < 0.05), whereas no significant differences were observed in the home-based (Systolic: 130 ± 4 vs. 126 ± 3 mmHg; diastolic: 73 ± 3 vs. 71 ± 3 mmHg) and control groups (p > 0.05). Supervised handgrip exercise also reduced central BP systolic (120 ± 5 vs. 109 ± 5 mmHg), diastolic (73 ± 2 vs. 67 ± 2 mmHg); and mean BP (93 ± 3 vs. 84 ± 3 mmHg), whereas no significant effects were found in the home-based (Systolic: 119 ± 4 vs. 115 ± 3 mmHg; Diastolic: 74 ± 3 vs. 71 ± 3 mmHg) and control groups (p > 0.05). In conclusion, supervised, but not home-based, isometric training lowered brachial and central BP in hypertensives.
Melanoma Research | 2017
Fernando Pinheiro de Souza Neto; Sara Santos Bernardes; Poliana Camila Marinello; Gabriella Pasqual Melo; Rodrigo Cabral Luiz; Rubens Cecchini; Alessandra Lourenço Cecchini
Cutaneous melanoma is one of the most lethal cancers because of its increased rate of metastasis and resistance to available therapeutic options. Early studies indicate that metformin has beneficial effects on some types of cancer, including melanoma. To clarify knowledge of the mechanism of action of metformin on this disease, two treatment-based approaches are presented using metformin on melanoma progression: an in-vitro and an in-vivo model. The in-vitro assay was performed for two experimental treatment periods (24 and 48 h) at different metformin concentrations. The results showed that metformin decreased cell viability, reduced proliferation, and apoptosis was a major event 48 h after treating B16F10 cells. Oxidative stress was characterized by the decrease in total thiol antioxidants immediately following 24 h of metformin treatment and showed an increase in lipid peroxidation. The in-vivo model was performed by injecting B16F10 cells into the subcutaneous of C57/BL6 mice. Treatment with metformin began on day 3 and on day 14, the mice were killed. Treatment of mice with metformin reduced tumor growth by 54% of its original volume compared with nontreatment. The decrease in systemic vascular endothelial growth factor, restoration of antioxidants glutathione and catalase, and normal levels of lipid peroxidation indicate an improved outcome for melanoma following metformin treatment, meeting a need for new strategies in the treatment of melanoma.
Tumor Biology | 2016
Poliana Camila Marinello; Thamara Nishida Xavier da Silva; Carolina Panis; Amanda Fouto Neves; Kaliana Larissa Machado; Fernando H. Borges; Flávia Alessandra Guarnier; Sara Santos Bernardes; Julio Cesar Madureira de-Freitas-Junior; José Andrés Morgado-Díaz; Rodrigo Cabral Luiz; Rubens Cecchini; Alessandra Lourenço Cecchini
Journal of Cancer Survivorship | 2017
Camila S. Padilha; Poliana Camila Marinello; Daniel A. Galvão; Robert U. Newton; Fernando H. Borges; Fernando Tadeu Trevisan Frajacomo; Rafael Deminice
Cell and Tissue Research | 2014
Sara Santos Bernardes; Flávia Alessandra Guarnier; Poliana Camila Marinello; André Armani; Andréa Name Colado Simão; Rubens Cecchini; Alessandra Lourenço Cecchini
Revista de Patologia Tropical | 2015
Matheus Azevedo Barbosa; Leônidas Gomes Angelin; Gustavo Issamu Asai Saikawa; Cayo Julius Cesar de Oliveira; Suelen Santos da Silva; Jeanne Weber Vendruscolo; Poliana Camila Marinello; Thiago Cezar Fujita; Sérgio Paulo Dejato da Rocha; Maria Angelica Ehara Watanabe; Regina Mitsuka-Breganó; Idessania Nazareth Costa
American Journal of Immunology | 2014
Poliana Camila Marinello; Kaliana Larissa Machado; Rubens Cecchini; Alessandra Lourenço Cecchini