Prabowo Purwono Putro

Influence of feed intake on blood chemistry parameters in Kacang goats

Influence of feed intake on blood chemistry parameters in Kacang goats was studied. Nine clinically healthy female adult goats were used in this study. Animals were kept in individual box for 8 weeks. During the first 4 weeks the animals were fed at the level of about 40% of the feed requirement for maintenance plus medium activity (the restricted feeding period) and during the 5th-8th week (the fullfeeding period) they were offered full feed intake for maintenance plus medium activity (dry matter intake at the level of 3% of body weight). Feed intake was measured daily and the body weight was measured weekly. Blood samples were collected at the end of the 4th week and the 8th week for blood chemistry analyses. Paired t-test was used to evaluate the effects of feed intake on blood chemistry parameters. Restricted feeding (0.320±0.065 kg dry matter/d) for 4 weeks in clinically healthy female adult Kacang goats resulted in a decrease of body weight gain as well as serum calcium and protein concentrations, b...

Pelacakan Virus Bovine Viral Diarrhea pada Darah yang Dikoleksi dengan Kertas Saring Flinders Technology AssociatesTM (DETECTION OF BOVINE VIRAL DIARRHEA VIRUS FROM BLOOD SAMPLES COLLECTED USING FLINDERS TECHNOLOGY ASSOCIATESTM CARDS)

Bovine viral diarrhea virus (BVDV) is a common pathogen that causes major economic losses to dairyindustry worldwide. Amplification of 5’-UTR regions of BVDV genome from blood samples usingreversetranscriptase polymerase chain reaction (RT-PCR) is one of sensitive and accurate techniques forBVDV detection. A common limitation for molecular detection of virus is the ability to obtain high qualityof nucleic acid in the samples. Inappropriate storage conditions will result in a false negative test. FiveEDTA-blood samples collected from BVDV persistently infected cattle were used in the present study.Ten repetitions were done for each sample. The blood were then dripped in the middle part of the FlindersTechnology AssociatesTM (FTA) cards and let it dried for approximately 2 hours. The effectiveness of theFTATMcards for storage and retrieval of BVDV RNA from blood samples molecularly using RT-PCRtechnique were tested and evaluated. The results proved that FTATM cards are effective and safe for thestorage of BVDV genome for a long period at room temperature. Based on the result of the present study,it can be concluded that in veterinary medicine field, the application of FTATM technology needs to bedeveloped to various type of samples and should be used routinely for collecting samples containinginfectious agents.