Priscila Alves Dias

Campylobacter species isolated from poultry and humans, and their analysis using PFGE in southern Brazil.

Campylobacteriosis is a bacterial disease transmitted to humans through ingestion of contaminated food. Six hundred samples were collected, 200 from human stool samples, 200 from poultry products and 200 from poultry feces in Southern Brazil, and then inoculated on blood agar plates. A total of 58% of the poultry feces, 17% of the poultry meat, and 2% the of human stools tested positive for Campylobacter. Positive Campylobacter colonies were identified as Campylobacter jejuni or Campylobactercoli by multiplex PCR. Campylobacter isolates were analyzed using PFGE to compare different profiles according to the source. This study demonstrated that there are different Campylobacter clones distributed in different aviaries in Southern Brazil. In addition, PFGE molecular profiles suggested that broilers can be a source of contamination of poultry products. However, the human isolate studied did not show any relationship with other strains examined.

Propriedades antimicrobianas do kefir

The fermented milks have been consumed since the antiquity, and currently it have been widely marketed and consumed around the world. Kefir is a fermented, acid, mildly alcoholic homemade milk produced from the fermentation of milk by kefir grains which contain a stable population of microorganisms. The fermentation process generates a series of compounds which impart flavor and aroma to kefir, and bioactive substances responsible for nutraceutic properties. The use of micro-organisms with antimicrobial properties as natural preservative is an alternative that has the advantage of inhibiting the development of pathogenic and spoilage bacteria without the use of undesirable chemicals. The interest in these species has increased in recent years, enabling the use of these probiotic micro-organisms as preservatives and natural inhibitors in several animal products such as milk, meat and their products. The aim of this paper is to present a review of literature on the antimicrobial properties attributed to kefir.

Primeiro relato de Salmonella enterica e Campylobacter spp. isolados de Garibaldi (Chrysomus ruficapillus) e Canário-da-terra (Sicalis flaveola) silvestres

Some species of wild birds have been identified as reservoirs of Campylobacter e Salmonella, they may exert as disseminators of these microorganisms. However, in Brazil, there is no study on the role of wild birds in the transmission of these two pathogens. This study aimed at searching the occurrence of Campylobacter and Salmonella in wild birds that feed in the rice paddies. By using mist nets 23 garibaldis (Chrysomus ruficapillus), one rolinha-picui (Columbina picui) and one canario-da-terra (Sicalis flaveola) were captured. The birds stool samples were collected using swabs, and they were processed for searching Campylobacter spp. and Salmonella enterica. Eight (32 %) fecal samples collected from C. ruficapillus were Campylobacter-positive and six (24 %) were positive to Salmonella enterica; five (20 %) samples collected from C. ruficapillus and one (4%) from S. flaveola were positive to Salmonella enterica. The two birds C. ruficapillus and S. flaveola showed to be reservoirs of Campylobacter and Salmonella, and they can be potential transmitters of these pathogens. This study is the first report on the isolation of Campylobacter and Salmonella from wild birds C. ruficapillus and S. flaveola.