Qais Banihani

Toxicity of copper(II) ions to microorganisms in biological wastewater treatment systems

Copper is an essential element, however, this heavy metal is an inhibitor of microbial activity at relatively low concentrations. The objective of this study was to evaluate the inhibitory effect of copper(II) towards various microbial trophic groups responsible for the removal of organic constituents and nutrients in wastewater treatment processes. The results of the batch bioassays indicated that copper(II) caused severe inhibition of key microbial populations in wastewater treatment systems. Denitrifying bacteria were found to be very sensitive to the presence of copper(II). The concentrations of copper(II) causing 50% inhibition (IC(50)) on the metabolic activity of denitrifiers was 0.95 mg L(-1). Copper was also inhibitory to fermentative bacteria, aerobic glucose-degrading heterotrophs, and nitrifying bacteria (IC(50) values=3.5, 4.6 and 26.5 mg L(-1), respectively). Nonetheless, denitrifying and nitrifying bacteria showed considerable recovery of their metabolic activity after only several days of exposure to high copper levels (up to 25 and 100mg Cu(II) L(-1) for denitrification and nitrification, respectively). The recovery could be due to attenuation of soluble copper or to microbial adaptation.

Toxicity of fluoride to microorganisms in biological wastewater treatment systems.

Fluoride is a common contaminant in a variety of industrial wastewaters. Available information on the potential toxicity of fluoride to microorganisms implicated in biological wastewater treatment is very limited. The objective of this study was to evaluate the inhibitory effect of fluoride towards the main microbial populations responsible for the removal of organic constituents and nutrients in wastewater treatment processes. The results of short-term batch bioassays indicated that the toxicity of sodium fluoride varied widely depending on the microbial population. Anaerobic microorganisms involved in various metabolic steps of anaerobic digestion processes were found to be very sensitive to the presence of fluoride. The concentrations of fluoride causing 50% metabolic inhibition (IC(50)) of propionate- and butyrate-degrading microorganisms as well as mesophilic and thermophilic acetate-utilizing methanogens ranged from 18 to 43 mg/L. Fluoride was also inhibitory to nitrification, albeit at relatively high levels (IC(50)=149 mg/L). Nitrifying bacteria appeared to adapt rapidly to fluoride, and a near complete recovery of their metabolic activity was observed after only 4d of exposure to high fluoride levels (up to 500 mg/L). All other microbial populations evaluated in this study, i.e., glucose fermenters, aerobic glucose-degrading heterotrophs, denitrifying bacteria, and H(2)-utilizing methanogens, tolerated fluoride at very high concentrations (>500 mg/L).

Stoichiometric and molecular evidence for the enrichment of anaerobic ammonium oxidizing bacteria from wastewater treatment plant sludge samples.

Anammox enrichments were readily developed from seven municipal wastewater treatment plants (WWTPs) sludge, but not with methanogenic granular sludge from two agro-industrial WWTPs. Only 50d was required for the first evidence of anammox activity from a return activated sludge obtained from a WWTP operated for nutrient removal. The molar ratios of nitrite and ammonium consumption of approximately 1.32 as well as nitrate and dinitrogen gas product ratios of approximately 0.095 provided evidence of the anammox reaction. The presence of anammox was confirmed by polymerase chain reaction (PCR) using primer sets (PLA46F and AMX820R) specific for anammox bacteria. The 16S rRNA gene fragment of anammox bacteria was detected in seven enrichment cultures (ECs) with demonstrated anammox activity but not in the original inocula from which the ECs were derived and also not in the two methanogenic sludge samples, which indicates the PCR predicted the anammox activity. Two genera, Brocadia and Kuenenia, were successfully identified as the Planctomycetes occurring in the clone libraries of successful anammox enrichments. Brocadia dominated in cultures that were respiked extensively; whereas Kuenenia predominated in cultures that were less aggressively respiked. These findings indicate that respiking management may play an important role on selecting the genus of anammox bacteria. The batch enrichment results clearly illustrate that anammox can be readily enriched from municipal sludge from a wide variety of process operations at WWTPs.

Fate of Polybrominated Diphenyl Ethers during Wastewater Treatment/Polishing and Sludge Stabilization/Disposal

Large quantities of polybrominated diphenyl ethers (PBDEs) have been used as flame retardants in clothing and plastic products since the 1970s. A small fraction of the PBDEs in manufactured products subsequently enters municipal wastewater. Nevertheless, the resistance of these compounds to chemical and biochemical transformations provides opportunities for accumulation in sediments that are in contact with wastewater effluent and agricultural soils that are amended with biosolids derived from wastewater treatment. Balances developed for PBDE congeners indicate that conventional wastewater treatment processes and soil infiltration of treated wastewater in recharge operations do not discriminate significantly among the major congeners in commercially available PBDE products. Accumulation of PBDEs at near part‐per‐million levels was measured in the surface sediments at the Sweetwater Recharge Facility in Tucson, Arizona, during 10–15 years of operation. Half‐lives for loss of major PBDE congeners from sediments were decades or longer. Local agricultural soils amended with biosolids over a 20‐year period showed similar accumulation of PBDEs. The widespread use of PBDEs in commercial products, compound persistence, and toxicity indicate that additional effort is warranted to better understand fate‐determining processes for PBDEs in the environment.

Treatment of high-strength synthetic sewage in a laboratory-scale upflow anaerobic sludge bed (UASB) with aerobic activated sludge (AS) post-treatment

Performance of a combined system up-flow anaerobic sludge blanket (UASB) followed by aerobic treatment activated sludge (AS) for removal of carbonaceous and nitrogenous contaminants at an average temperature of 25°C was investigated. The combined system was fed with high strength synthetic sewage having chemical oxygen demand (COD) of 2500 mg L−1. The organic loading rate (OLR) of the UASB reactor was increased gradually from 1.1 to 3.8 gCOD Lr −1 d−1. At steady state condition, the UASB reactor achieved removal efficiency up to 83.5% of total COD (CODtot), 74.0% of volatile fatty acid (VFA) and 94.0% of protein. The combined system performed an excellent organic removal pushing the overall removal efficiency of CODtot, VFA and protein to 91.0%, 99.9% and 98.2%, respectively. When the OLR of the UASB increased to 4.4 g COD Lr −1 d−1, the UASB was overloaded and; thus, its effluent quality deteriorated. In respect to nitrogen removal, both partial nitrification and complete nitrification took place in aerobic post-treatment. When the dissolved oxygen (DO) concentration was >2.0 mg L−1, complete nitrification (period B) occurred with an average nitrification efficiency of 96.2%. The partial nitrification occurred due to high OLR to AS during the overloading event (period A) and when DO concentration was <2.0 mg L−1 (period C). The maximum accumulated nitrite concentration in periods A, B and C were 90.0, 0.9 and 75.8 mg NO− 2 -N L−1, respectively. The nitrogen balance results of periods A and C indicated that there was a discrepancy between the amount of ammonium nitrogen removed and the amount of oxidized nitrogen formed. This suggests the occurrence of simultaneous nitrification/denitrification (SND) in aerobic post-treatment.