Qiao Mingqi

Studies on the Metabonomics of Premenstrual Syndrome Liver-Qi Invasion Patient Interfered by Jingqianping Granules

Abstract This work deals with the variation of urinary metabolite of patients suffering from premenstrual syndrome (PMS) and the function of Jingqianping granules (JG) in intervening liver-Qi invasion by metabonomic. The metabonomic characteristics and the micromolecular marking compound, which are closely related to the pathogenesis of PMS liver-Qi invasion cases, are also investigated. PMS liver-Qi invasion cases are calibrated according to the diagnostic criteria, including criteria, and excluding criteria. The urine samples from PMS liver-Qi invasion patients are analyzed at different times using UPLC-Q-TOF in combination with PCA pattern analysis, followed by discrimination of the metabolic outline and searching for the possible biological tags and metabolic pathways. The metabolic outline of premenstrual urine samples has significant difference among the control group, the PMS liver-Qi invasion group, and the PMS liver-Qi invasion treatment group. The metabolic outline of premenstrual urine sample of the PMS liver-Qi invasion group diverges from the premenstrual and postmenstrual urine samples of the control group and the postmenstrual urine sample of the PMS liver-Qi invasion group. Compared with the control group, N-acetyl-2-glutamate-5-semialdehyde has decreased greatly in the N-acetyl-2-glutamate-5-semialdehyde liver-Qi invasion group, and histidine and 3-methoxy-4-hydroxymandelaldehyde have increased significantly. This research, for the first time, deals with the metabolic pattern differences between the control group and the administration group. It shows that JG may repair metabolic disturbance of endogenous micromolecules in PMS liver-Qi invasion cases in terms of metabonomics. It also demonstrates that symptoms present before menstruation in PMS patients and the characteristic of PMS is that the symptoms appear before menstruation and disappear after it among PMS liver-Qi invasion cases.

Dynamic changes in serum estradiol and progesterone levels in patients of premenstrual syndrome with adverse flow of liver-qi.

Objective To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome (PMS) and reveal partial microcosmic mechanisms of adverse flow of liver- qi . Methods Estradiol (E 2 ) and progesterone (P) levels in serum were determined at different phases of menstrual cycle by radioimmunoassay. Results In the group of PMS with adverse flow of liver- qi , the secretive peak value of E 2 and P at the follicular phase significantly decreased, and the secretive peak value at the luteal phase did not come into being. Conclusions Low E 2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver- qi . One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E 2 and P.

Behavioral Observation and Analysis of PMS Liver-qi Depression Rat Model Established by Chronic Restraint Stress and Resident-Intruder Paradigm

Abstract This study was aimed at investigating the feasibility of establishing premenstrual syndrome (PMS) liver-qi depression rat model with resident-intruder paradigm compared to chronic restraint stress. The PMS liver-qi depression rat model was prepared with chronic restraint stress and resident-intruder paradigm. The effect was systematically evaluated through behavioral methods such as open-field test and tail suspension test. Then, results from both groups were compared. The results showed that both chronic restraint stress and resident-intruder paradigm can effectively simulate the state of premenstrual depression and postmenstrual disappearance. The results of the open-field test suggested that there was no significant difference between these two methods (P > 0.05). It was concluded that as a social psychological stress method the resident-intruder paradigm can preferably simulate the process of clinical PMS liver-qi depression caused by emotional abnormality and overcome the disadvantage of obvious body stress of the chronic restraint stress method. But the success ratio was too low, and further inspection was needed to examine its stability.

Empirical Study of Changes of Pre-Menstrual Syndrome Model Rats with Liver-qi Invasion and Liver-qi Depression in Peripheral Blood, Sexual Hormones, Different Encephalic Regions and Accommodate Hormones

Abstract This work successfully used model rats with Pre-Menstrual Syndrome (PMS) liver-qi invasion and liver-qi depression in the early development days to detect the content levels of estradiol (E2), progesterone (P) and prolactin (PRL) in their blood serums and different encephalic regions, to explore the micro-mechanism of the syndrome of the liver failing to maintain the normal flow of qi. 40 rats with estrous cycles not in accepting time were selected and divided randomly into five groups: the normal control group, PMS liver-qi invasion model group, PMS liver-qi depression model group, PMS liver-qi invasion medication-administered group and PMS liver-qi depression medication-administered group. Emotional stimulation and multiple factors combination were used to prepare the PMS liver-qi invasion model. Chronic stress constraints were used to prepare the PMS liver-qi depression model. Jingqianping granules were administered to rats to interfere with the PMS liver-qi invasion mode, and Jingqianshu granules were administered to rats to interfere with the PMS liver-qi depression mode. Radioimmunity was used to determine the content levels of E2, P and PRL in the blood serum and centrum, and to compare and analyze the differences among the five groups. In the open-field experiment, the crossing scores, rearing scores and open-field experiment total scores of rats in the PMS liver-qi invasion model group increased remarkably (P

HPLC-ESI-MS n 法鉴定大鼠口服白芍提取物后血清及脑内的化学成分

目的 定性分析白芍提取物在大鼠血清及脑组织中的化学成分。 方法 大鼠ig给予白芍提取物后，采集血液并分离脑组织部位，采用HPLC-ESI-MSn法检测入血及分布于脑组织中的化学成分。 结果 通过相应保留时间和质谱数据，以及与文献资料及对照品比对，在大鼠含药血清中共检测到34个化学成分，其中8个为新代谢产物；在全脑组织中共检测到3个化学成分，其中1个为新代谢产物。 结论 建立的HPLC-ESI-MSn方法有助于阐明白芍提取物抗抑郁作用的药效物质基础。