R. Daghfous

Érythème pigmenté fixe à la sulfaguanidine

Resume Introduction L’erytheme pigmente fixe est une eruption consideree comme exclusivement medicamenteuse. Les classes de medicaments les plus incriminees sont les sulfamides, les tetracyclines et les pyrazoles. Nous rapportons 9 cas d’erytheme pigmente fixe a la sulfaguanidine qui est un sulfamide peu resorbe par voie orale. Observations Tous les malades ont eu un ou plusieurs episodes de reactivation d’erytheme pigmente fixe consecutifs a une automedication a la sulfaguanidine pour une diarrhee. Le nombre des lesions lors des recidives a augmente chez 7 malades sur 9. Le delai de reactivation des lesions a ete raccourci au fur et a mesure des poussees. Les mains ont ete les plus touchees par ces lesions (8 cas sur 9). Discussion La sulfaguanidine doit etre ajoutee a la liste des medicaments peu resorbes par voie digestive et qui peuvent induire un erytheme pigmente fixe.

DRESS Syndrome Following Levofloxacin Exposure With Positive Patch-test

Drug rash with eosinophilia and systemic symptoms (DRESS syndrome) in a severe cutaneous drug reaction, which can be life threatening. Levofloxacin has not been reported in literature as a causative drug. We are presenting an exceptional case of levofloxacin-induced DRESS without eosinophilia and with positive patch-tests to levofloxacin.

Probable disseminated BCG infection in a 10-month-old child after BCG vaccination

The disseminated BCG infection is a rare and serious complication of bacillus Calmette-Guerin (BCG) vaccine. We report a case of probable disseminated BCG infection in a 10-month-old child who had been vaccinated by BCG vaccine at birth. No incident was noted on the first days of vaccination. At the age of 40 days, she developed left supra clavicular and axillar abcessed lymph nodes. She was treated by surgical flattening of the lymph adenopathy. The same lymph node recurred at the age of 6 months and was treated with surgery. Few weeks after the second episode, cutaneous ulcerative lesions appeared. Cutaneous biopsy was performed and showed lesions compatible with tuberculosis. X-rays showed osteolysis of P1 of the middle finger in the left hand. She was treated by anti-tuberculosis antibiotics.

Lichen Associated with Metformin

We report an exceptional case of bullous lichen induced by metformin in a patient with diabetes mellitus.

Multiple Drug Intolerance Syndrome in an Epileptic Woman

The term multiple drug intolerance syndrome (MDIS) is used for patients who express adverse drug reactions to three or more drugs without a known immunological mechanism [1]. It is a distinct clinical entity, different from cross-reactivity and flare-up reactions. The symptoms can range from a benign rash to life threatening syndromes like drug reaction with eosinophilia and systemic symptoms (DRESS syndrome). We report a case of an epileptic woman who presented MDIS. The case was notified to the Tunisian pharmacovigilance center on February 20th 2014.

Bullous Eruption Associated With Dihydropyridines With Cross Reactivity.

The spectrum of cutaneous eruptions associated with dihydropyridines is extensive, varying from exanthemas to severe adverse events. We report a case of bullous eruption, one month after starting nicardipine and lercanidipine. The same symptoms recurred few days after taking nitrendipine.

Fixed Drug Eruption Related to Tetracyclines

withdrawn A1.02 BDNF Mediated Angiogenesis Potential is Decreased Associated with Aging D. Cai, L. Cao, S. Chen, D. Li, X. Shen, X Zheng and X. Liu Ji Nan University, Key Laboratory for Regenerative Medicine, Minstry of Education, Guangzhou, China The mechanism of age-related decrease of angiogenic potential in myocardium is still unclear. Cardiac microvascular endothelial cells (CMECs) play a key role in cardiac angiogenesis. In this study, using the CMECs which are isolated from young and old hearts, we found that the migration and proliferation capacity of old CMECs were diminished. BDNF was able to increase the migration and proliferation of CMECs no matter in young and old CMECs, however, the effects in old CMECs was less potent compared with the young CMECs. In vivo study showed that delivery of BDNF in young heart in ischemic situation was able to increase the vessel numbers in both infarct and border zone significantly, but not in young heart in non-ischemic situation. The microarray results showed that 84 genes were up-regulated, while 81 genes were down-regulated upon BDNF treatment. The functional annotations of genes are cell migration, blood vessel morphogenesis, angiogenesis, regulation of proliferation, cell cycle regulation, etc, which have been shown the strong potential effects in migration, proliferation and angiogenesis. The results of present study revealed that BDNF-TrkB pathway play an important role in angiogenesis of myocardium. Although CMECs express BDNF consistently, however, BDNF might not initiate the angiogenesis in heart individually in vivo. BDNF-mediated angiogenic potential might depend on the cross talk with focal micro-environment. Importantly, senescence of CMECs was able to impair the BDNF-mediated migration and proliferation capacity. It might contribute to age-related decrease of angiogenic potential in myocardium and poor regenerative capacity seen in aged heart. A1.03 Memory enhancing effects of saffron in adult & aged mice are correlated with the antioxidant protection: In vitro and in vivo studies M. Papandreou, M. Tsachaki, S. Efthimiopoulos, P. Cordopatis, F. Lamari and M. Margarity University of Patras, Biology, Lab. Human & Animal Physiology, Patras, Greece, University of Athens, Biology, Lab. Animal & Human Physiology, Athens, Greece, University of Athens, Biology, Animal & Human Physiology, Athens, Greece, University of Patras, Pharmacy, Lab. Pharmacognosy & Chemistry of Natural Products, Patras, Greece Oxidative stress is implicated in senescence and age-related pathologies, with memory deficits as the commonest manifestations. Herbal ingredients are sought to forestall/reverse those deficits as dietary components/or supplements. The effect on cognitive function of a 7-day, intraperitoneal administration of saffron was examined in healthy adult and aged mice by step through test. Results showed that saffron-treated mice exhibited significant improvement in learning and memory. Experiments in whole brain homogenates revealed that saffron administration resulted in significantly lower brain lipid peroxidation (malondialdehyde, 44–63%) and higher antioxidant parameters (glutathione, ascorbic acid, total antioxidant power). Saltand detergent-soluble AChE activity was significantly decreased only in adult mice. Thus, the significant cognitive enhancement conferred by saffron administration in adult and aged mice, is closely related to the antioxidant reinforcement; AChE inhibition (in adult mice) plays also a minor role. Studying further the antioxidant potential, the effect(s) of saffron and crocetin (main crocin metabolite), were examined against H2O2-induced toxicity in SH-SY5Y and HEK293 cells. Cell viability and scavenging of free radicals after co-treatment with H2O2 (250–750 lM) and the tested compounds (1–250 lg/ml saffron, 1–125 lM crocetin) were determined with MTT and DCF assays. Results showed that saffron and crocetin provide strong protection in rescuing cell viability and repressing ROS production in the SH-SY5Y cells; moderate effects in HEK293 cells. Considering, thus, earlier metabolic studies, crocetin appears to be responsible for the in vivo effects. A1.04 Protective effects of triphlorethol-A against formaldehyde-induced oxidative damage and apoptosis: role of mitochondria-mediated caspase-dependent pathway R. Zhang, K. A. Kang, M. J. Piao, K. C. Kim, J. Y. Choi, J. Choi, J. Park and J. W. Hyun School of Medicine, Jeju National University, Jejusi, Republic of Korea, Department of Pharmacology, School of Medicine, Ewha Womans University, Seoul, Republic of Korea, Faculty of Environmental Engineering, University of Seoul, Seoul, Republic of Korea, Division of Hematology and Oncology, Department of Internal Medicine, Gachon University of Medicine Science, Gil Hospital, Incheon, Republic of Korea The toxicity of formaldehyde (HCHO) has been attributed to its ability to form adducts with DNA and proteins. Triphlorethol-A, derived from Ecklonia cava, was reported to exert a cytoprotective effect against oxidative stress damage via an antioxidant mechanism. The aim of this study was to examine the mechanisms underlying triphlorethol-A ability to protect Chinese hamster lung fibroblast (V79-4) cells against HCHOinduced damage. Triphlorethol-A significantly decreased the HCHO-induced intracellular reactive oxygen species (ROS) production. Triphlorethol-A prevented increased cell damage induced by HCHO via inhibition of mitochondria-mediated caspase-dependent apoptosis pathway. Triphlorethol-A diminished HCHO-induced mitochondrial dysfunction including loss of mitochondrial membrane action potential (Y) and adenosine triphosphate (ATP) depletion. Furthermore, the anti-apoptotic effect of triphlorethol-A was exerted through inhibition of c-Jun NH2-terminal kinase (JNK) which was enhanced by HCHO. Our data indicate that triphlorethol-A exerts a cytoprotective effect in V79-4 cells against HCHO-induced oxidative stress by inhibiting the mitochondria-mediated caspase-dependent apoptotic pathway. FEBS Journal 277 (Suppl. 1) 37–271 (2010) a 2010 The Authors Journal compilation a 2010 Federation of European Biochemical Societies 37 A1 – Ageing Abstracts