R. Jailler

Effects of age and breed of beef bulls on growth parameters, carcass and muscle characteristics

The effects of age and breed on growth parameters, carcass and muscle characteristics of bulls, slaughtered at 15, 19 and 24 months of age, were analysed in four French breeds: Aubrac (AU), Charolais (CH), Limousin (LI), and Salers (SA). Muscle characteristics were determined in three muscles: longissimus thoracis (LT), semitendinosus (ST) and triceps brachii (TB). They included: (1) the % frequency, cross-sectional area and % area of fibre types, which were classified according to the contractile nature of the fibres and their metabolic properties (SO slow oxidative, FOG fast oxidative glycolytic and FG fast glycolytic); (2) the isocitrate dehydrogenase (ICDH) and lactate dehydrogenase (LDH) activities, representative of oxidative and glycolytic metabolism respectively; and (3) the total and insoluble collagen contents. In the four breeds, the average daily gain and the food efficiency decreased with age ( P P P P P P P P P So carcass characteristics were modified between 15 and 24 months, and muscle characteristics were especially modified from 19 months of age. In addition, differences in slaughter data between breeds were clear and consistent, whereas those of muscle characteristics were few and not consistent.

Effect of season on contractile and metabolic properties of desert camel muscle (Camelus dromedarius)

Thirty fattened one humped desert camels were used to examine the effect of season on contractile and metabolic properties of Longissimus thoracis (LT) muscle. Ten camels were slaughtered according to seasons of the year (winter, summer and autumn). Season significantly influenced muscle chemical composition, ultimate pH (pHu) and color. Activities of metabolic enzymes were higher during autumn season compared to summer and winter for phosphofructokinase (+64% compared to both seasons) and for isocitrate dehydrogenase (+35% and +145% in autumn vs. summer and winter, respectively). Quantification of muscle myosin heavy chain isoforms by SDS-PAGE electrophoresis showed only presence of type I and type IIa MyHC in camel muscle and indicated high proportion in winter for type I and in autumn for type IIa with respect to other seasons. Several correlations between different MyHC proportions and enzyme activities were reported. These findings indicated that muscle characteristics in camels are influenced by season.

Young Salers suckled bull production: effect of diet on performance, carcass and muscle characteristics and meat quality

The aim of this work was to improve the knowledge on young suckled Salers bull production and to study the effect of forage type and concentrate level on performance, carcass and muscle characteristics as well as on meat quality. Twenty-four Salers male calves of 150 days of age were assigned to six groups: C0 (fed exclusively with hay and dams milk and slaughtered at approximately 6 months of age), and HH (hay - high concentrate), HL (hay - low concentrate), GH (cut grass - high concentrate), GL (cut grass - low concentrate) and CP (control pasture: pasture - high concentrate) groups differing in feeds received until slaughter and slaughtered unweaned at approximately 10 months of age. Carcass weights averaged 210 kg at 10 months of age at slaughter. Average daily weight gain (ADWG) in HH and GH groups tended to be higher (P = 0.09) than in HL and GL groups (1354 v. 1248 g/day). ADWG in CP group (1542 g/day) was higher (P < 0.05) than in the other groups. Carcass weight in CP group (230 kg) tended to be higher (P < 0.1) than in HL (198 kg) and GL (200 kg) groups. Carcass muscle weight was higher (P < 0.05) in GH (155 kg) and CP groups (165 kg) than in HL (141 kg) and GL (142 kg) groups. Carcass and offal fatty tissue weights and carcass fatness did not differ between groups. Neither forage type nor concentrate level had significant effect on the area of muscle fibres or on muscle metabolic enzyme activities (namely, lactate dehydrogenase - LDH, phosphofructokinase - PFK, isocitrate dehydrogenase - ICDH, citrate synthase - CS and cytochrome-c oxidase - COX). semitendinosus muscle of CP group presented higher CS enzyme activities (8.10 μmol/min per g) than HH (5.30 μmol/min per g) and GL (4.52 μmol/min per g) groups. Neither total nor insoluble collagen content significantly differed between groups. Lipid content in rectus abdominis muscle was relatively low (average 67.5 mg/g dry matter) and was not affected by diet (P > 0.05). The ratio between n-6 and n-3 polyunsaturated fatty acid content was lower (P = 0.01) in the low-concentrate-fed than in the high-concentrate-fed group (3.95 v. 5.37, respectively). Sensory analysis noted that longissimus thoracis muscle from CP animals was more tender and juicy than that from HH and GH animals (P < 0.05).

Effects of hay quality on intake, growth path, body composition and muscle characteristics of Salers heifers

A comparison between continuously (CG group, n = 9) and discontinuously (DG, n = 14) growing Salers heifers during a two year growth period after weaning was performed. Feed was available to all animals ad libitum throughout the experiment. Growth differences in the winter resulted from a different quality of the offered hay. In the summer, the animals were conducted indoors and offered the same good quality hay. Serial slaughter was carried out in order to estimate the evolution of body composition and muscle characteristics (fibre type and area, collagen) which may influence meat quality. This experimental scheme made it possible to simulate two types of nutritional environment (poor and rich) and to study their impact on intake, growth, body composition and muscle characteristics of the animals. DG heifers always underwent a summer compensatory growth, even though not complete, when compared with CG animals. The final slaughter was carried out at the same live weight, approximately 700 kg. On average, DG heifers were therefore slaughtered 56 days later than CG animals. The differences in mean daily weight gains always neared 300 g.d–1, respectively in favour of CG in the winter and DG in the summer. Growth compensation was attributable to an increased intake (between +6 and +7%) in the summer and a tendency for a higher growth efficiency of DG animals. No significant major difference was detected between CG and DG body composition at the final slaughter at which final carcass was approximately 400 kg and total adipose tissue weight reached approximately 130 kg (21.5% of the empty body weight). Final muscle characteristics were not modified by growth type either. It was concluded that growth path can vary according to food availability, without affecting the characteristics of the finished carcasses at constant weight. growth path / heifer / body composition / muscle characteristics / hay intake * Corresponding author: hoch@vet-nantes.fr Present address: UMR ENV Nantes INRA Gestion de la Santé Animale, Atlanpole-Chantrerie, BP 40706, 44307 Nantes Cedex 03, France. Article published by EDP Sciences and available at http://www.edpsciences.org/animres or http://dx.doi.org/10.1051/animres:2005022

Influence of the diet and grazing on adipose tissue lipogenic activities and plasma leptin in steers.

The objectives of the two experiments were to determine the respective effects and interactions of diet type (grass v. maize diets) and physical activity (grazing v. zero grazing) on lipogenic enzyme activities and adipose cell size in subcutaneous, perirenal and intermuscular adipose tissues and on plasma metabolites and hormones in Charolais steers. After weaning, the steers were assigned to two (Experiment 1, n = 24) or three (Experiment 2, n = 24) groups, with steers in Experiment 1 grazed grass or indoors maize-silage-fed and steers in Experiment 2 grazed grass, indoors cut grass- or indoors maize-silage-fed. Both experiments lasted for 23 months. All grass-fed animals were fed grass silage during the two winter seasons. During the two summer seasons, steers fed on grass were rotationally grazed on a perennial rye-grass pasture while steers fed on cut grass were fed indoors on freshly cut grass alone. Steers fed on maize silage were fed maize silage indoors during the entire experiment. All animals were reared for similar body weight and growth rates and slaughtered at the same age (31 to 32 months). Activities of lipogenic enzymes were significantly lower in the three adipose tissue sites of steers fed cut grass compared with maize silage, although there were less-marked effects in intermuscular adipose tissue. Plasma insulin and glucose concentrations were also lower in steers fed cut grass whereas plasma leptin concentration was similar. As body fat content was not affected by nutritional treatment, it is suggested that the decrease in potential lipogenic activity was associated with the nature of the diet and not to differences in available net energy. In other respects, grazed grass compared with eating cut grass did not affect lipogenic enzyme activities but decreased plasma leptin concentrations in the older steers and increased plasma non-esterified fatty acids and glucose concentrations without affecting adipose tissue weight and adipose cell size.