Rafaela Cuenca

Clinical biochemical parameters of the endangered catalonian donkey breed: normal values and the influence of sex, age, and management practices effect

Twelve clinical biochemical parameters were determined in 97 animals of all age groups and both sexes of the endangered Catalonian donkey breed. Significant sex differences were observed for phospholipid concentration (P<0.01). Evaluating the effect of management practices on the various parameters showed significant differences for total bilirubin (P<0.001) and creatinine (P<0.05) concentrations and gamma-glutamyltransferase (P<0.05) activity. Moreover, it was observed that inorganic phosphorus concentration decreased with age (P<0.001), whereas albumin and triglyceride concentrations increased with age (P<0.01 and P<0.001, respectively). Comparison of biochemical ranges obtained for the Catalonian donkey breed with reference ranges for other breeds and populations (Mammoth, USA donkeys, UK donkeys, Indian donkeys and Poitou donkeys), indicated that most values were similar, with the exceptions of enzymatic activities mainly. The results reported in the present study could serve as reference ranges for donkey populations.

Antibodies to selected pathogens in wild boar (Sus scrofa) from Catalonia (NE Spain)

From 2004 to 2007, blood samples from 273 healthy wild boars (Sus scrofa), culled during the hunting season, were obtained in three areas of Catalonia (NE Spain): Pyrenees, Sant Llorenç del Munt i l’Obac Natural Park (SLM), and Ports de Tortosa i Beseit National Hunting Reserve (PTB). We investigated the presence of antibodies against classical swine fever virus (CSFV), African swine fever virus (ASFV), porcine vesicular disease virus (PVDV), porcine respiratory and reproductive syndrome virus (PRRSV), Aujeszky’s disease virus (ADV), porcine influenza A virus (PIV), porcine circovirus type 2 (PCV2), porcine parvovirus (PPV), Mycoplasma hyopneumoniae, Erysipelothrix rhusiopathiae, Salmonella spp., and Toxoplasma gondii. Four wild boars were suspicious for CSFV, but the infection was discarded with a virus neutralization test, and infection with a border disease virus was confirmed. Negative results were obtained against ASFV and PVDV. Antibodies were detected against PRRSV (3%), ADV (0.8%), PIV (6.4%), PCV2 (64.6%), PPV (54.7%), M. hyopneumoniae (26.6%), E. rhusiopathiae (5.3%), Salmonella spp. (11.3%), and T. gondii (43.5%). In SLM, we detected a higher seroprevalence for PIV and M. hyopneumoniae and a lower seroprevalence for E. rhusiopathiae than in the other two areas. In PTB, seroprevalence was higher for PPV, Salmonella spp., and PCV2. Adult wild boar displayed higher seroprevalence for PPV, PIV, and M. hyopneumoniae, whereas presence of antibodies for Salmonella spp. was higher in juveniles compared with adults and piglets.

Effect of a synthetic appeasing pheromone on behavioral, neuroendocrine, immune, and acute-phase perioperative stress responses in dogs

OBJECTIVE To study the effects of a synthetic, dog-appeasing pheromone (sDAP) on the behavioral, neuroendocrine, immune, and acute-phase perioperative stress responses in dogs undergoing elective orchiectomy or ovariohysterectomy. DESIGN Randomized, controlled clinical trial. ANIMALS 46 dogs housed in animal shelters and undergoing elective orchiectomy or ovariohysterectomy. PROCEDURES Intensive care unit cages were sprayed with sDAP solution or sham treated with the carrier used in the solution 20 minutes prior to use. Dogs (n = 24 and 22 in the sDAP and sham treatment exposure groups, respectively) were placed in treated cages for 30 minutes before and after surgery. Indicators of stress (ie, alterations in behavioral, neuroendocrine, immune, and acute-phase responses) were evaluated perioperatively. Behavioral response variables, salivary cortisol concentration, WBC count, and serum concentrations of glucose, prolactin, haptoglobin, and C-reactive protein were analyzed. RESULTS Behavioral response variables and serum prolactin concentration were influenced by sDAP exposure. Dogs exposed to sDAP were more likely to have alertness and visual exploration behaviors after surgery than were dogs exposed to sham treatment. Decreases in serum prolactin concentrations in response to perioperative stress were significantly smaller in dogs exposed to sDAP, compared with findings in dogs exposed to the sham treatment. Variables examined to evaluate the hypothalamic-pituitary-adrenal axis, immune system, and acute-phase responses were unaffected by treatment. CONCLUSIONS AND CLINICAL RELEVANCE sDAP appeared to affect behavioral and neuroendocrine perioperative stress responses by modification of lactotropic axis activity. Use of sDAP in a clinical setting may improve the recovery and welfare of dogs undergoing surgery.

Ultrasonographic imaging of loggerhead sea turtles (Caretta caretta)

Twenty live and five dead juvenile and subadult loggerhead sea turtles were examined ultrasonographically. Ten soft tissue areas of the integument were used as acoustic windows: cervical-dorsal and cervical-ventral, left and right cervicobrachial, left and right axillary, left and right prefemoral and left and right postfemoral windows. Anatomical cross-sections were performed on the dead turtles to provide reference data. The fourth and fifth cervical vertebrae, the spinal cord, and the venous sinuses of the external jugular vein were clearly visible through the cervical-dorsal acoustic window, and the oesophagus and the heart were imaged through the cervical-ventral acoustic window. The stomach was more frequently visible through the left axillary acoustic window. The liver could be imaged through both sides, but the right axillary acoustic window was better for visualising the gall bladder. The large and small intestines and the kidneys were visible through the right and left prefemoral acoustic windows; the kidneys were easily identified by their intense vasculature.

Evaluation of C-reactive protein, haptoglobin and cardiac troponin 1 levels in brachycephalic dogs with upper airway obstructive syndrome.

BackgroundBrachycephalic dogs have unique upper respiratory anatomy with abnormal breathing patterns similar to those in humans with obstructive sleep apnea syndrome (OSAS). The objective of this study was to evaluate the correlation between anatomical components, clinical signs and several biomarkers, used to determine systemic inflammation and myocardial damage (C-reactive protein, CRP; Haptoglobin, Hp; cardiac troponin I, cTnI), in dogs with brachycephalic upper airway obstructive syndrome (BAOS).ResultsFifty brachycephalic dogs were included in the study and the following information was studied: signalment, clinical signs, thoracic radiographs, blood work, ECG, components of BAOS, and CRP, Hp and cTnI levels. A high proportion of dogs with BAOS (88%) had gastrointestinal signs. The prevalence of anatomic components of BAOS was: elongated soft palate (100%), stenotic nares (96%), everted laryngeal saccules (32%) and tracheal hypoplasia (29.1%). Increased serum levels of biomarkers were found in a variable proportion of dogs: 14% (7/50) had values of CRP > 20 mg/L, 22.9% (11/48) had values of Hp > 3 g/L and 47.8% (22/46) had levels of cTnI > 0.05 ng/dl. Dogs with everted laryngeal saccules had more severe respiratory signs (p<0.02) and higher values of CRP (p<0.044). No other statistical association between biomarkers levels and severity of clinical signs was found.ConclusionsAccording to the low percentage of patients with elevated levels of CRP and Hp, BAOS does not seem to cause an evident systemic inflammatory status. Some degree of myocardial damage may occur in dogs with BAOS that can be detected by cTnI concentration.

Brucella species antibodies and isolation in wild boar in north-east Spain

BRUCELLOSIS is found in domestic and feral pigs around the world, and also in wild boar ( Sus scrofa ), and can be transmitted to human beings. It is considered to be a re-emerging disease in domestic pigs in Europe due to the increasing number of outbreaks on pig farms, often associated with

Fishhook lesions in loggerhead sea turtles.

This study describes four cases of loggerhead sea turtles with fishhooks in the gastrointestinal tract. Two dead turtles with a hook in the esophagus had local fibrosis with an invagination of the keratinized stratified squamous epithelium surrounding the hook, isolating it from the subjacent stroma, one had a hook in the cloaca which was expelled spontaneously, and one had plication of the intestine with necrosis caused by the long monofilament line attached to the hook lodged in the esophagus. Lethal injuries were related to the effect of strangulation and traction produced by the line throughout the gastrointestinal tract rather than the presence of the hook in the esophageal mucosa. Hook size, point of attachment to the gastrointestinal tract, the presence or not of a long monofilament line, and the traction applied by it could be crucial for turtle survival.

Reproductive status of captive Loggerhead sea turtles based on serum levels of gonadal steroid hormones, corticosterone and thyroxin.

Serum levels of gonadal steroid hormones, corticosterone and thyroxin (T(4)), were monitored monthly in two male and one female captive Loggerhead sea turtles (Caretta caretta) over a period of 12 months in 2004 and 3 months in 2006. Ovary ultrasonography was performed in April and July 2006. The turtles were kept together in an outdoor sea pool in natural temperature and photoperiod conditions from May to November, then in separate indoor pools from December to April. Circulating hormone levels were measured by radioimmunoassay. Oestradiol levels in the female turtle surged significantly in July, as did the progesterone level in September. Total testosterone levels were different in both males, but both peaked in September. The peaks of oestradiol in the female and testosterone in the males did not coincide, both showing delay with respect to the hormone cycle described in free-ranging Loggerhead sea turtles. A seasonal pattern in T(4) levels was not observed. The three captive turtles showed very low corticosterone levels throughout the year, with a September peak coinciding with the peaks of progesterone in females and testosterone in males. The results suggested that conditions of captivity should be as close as possible to natural conditions throughout the entire year since the process of vitellogenesis in this species is protracted. Exposure to natural conditions for only a few months is insufficient to induce reproduction/oviposition.

Haematology and blood biochemistry of capercaillie (Tetrao urogallus).

Haematological and blood biochemical parameters of 15 capercaillie {Tetrao urogallus) reared in captivity have been determined. Statistically significant age differences were observed in the following parameters: haemoglobin, MCHC, total bilirubin, triglycerides, alkaline phosphatase, potassium and total proteins.

Haematology and serum chemistry of Pyrenean chamois (Rupicapra pyrenaica) naturally infected with a border disease virus

In 2005 and 2006 an outbreak of disease associated with border disease virus (BDV) infection caused high mortality in the Pyrenean chamois (Rupicapra pyrenaica) in the Catalan Pyrenees (NE Spain). The aim of this study was to determine values for different haematological and serum biochemical analytes in 32 free-ranging Pyrenean chamois affected by the disease and to compare them with those obtained from healthy chamois. In the affected chamois red blood cell counts, haemoglobin concentrations, packed cell volumes, mean corpuscular volumes and lymphocyte counts were all lower, while the neutrophil and platelet counts were higher. Glucose, lactate, triglycerides, creatinine, total protein concentrations and alkaline phosphatase activity were also lower, in contrast to the concentrations of total bilirubin, urea and aspartate aminotransferase activity, which were higher. Most of the observed changes could be associated with cachexia and inflammation in the affected chamois. Lymphopenia could be directly related to the BDV, which would lead to immunosuppression and explain the high rate of secondary infection observed in these animals.