Rahmad Zakaria

Soils on exposed Sunda Shelf shaped biogeographic patterns in the equatorial forests of Southeast Asia

The marked biogeographic difference between western (Malay Peninsula and Sumatra) and eastern (Borneo) Sundaland is surprising given the long time that these areas have formed a single landmass. A dispersal barrier in the form of a dry savanna corridor during glacial maxima has been proposed to explain this disparity. However, the short duration of these dry savanna conditions make it an unlikely sole cause for the biogeographic pattern. An additional explanation might be related to the coarse sandy soils of central Sundaland. To test these two nonexclusive hypotheses, we performed a floristic cluster analysis based on 111 tree inventories from Peninsular Malaysia, Sumatra, and Borneo. We then identified the indicator genera for clusters that crossed the central Sundaland biogeographic boundary and those that did not cross and tested whether drought and coarse-soil tolerance of the indicator genera differed between them. We found 11 terminal floristic clusters, 10 occurring in Borneo, 5 in Sumatra, and 3 in Peninsular Malaysia. Indicator taxa of clusters that occurred across Sundaland had significantly higher coarse-soil tolerance than did those from clusters that occurred east or west of central Sundaland. For drought tolerance, no such pattern was detected. These results strongly suggest that exposed sandy sea-bed soils acted as a dispersal barrier in central Sundaland. However, we could not confirm the presence of a savanna corridor. This finding makes it clear that proposed biogeographic explanations for plant and animal distributions within Sundaland, including possible migration routes for early humans, need to be reevaluated.

Cryptic coloration of Macaranga bancana seedlings: A unique strategy for a pioneer species.

ABSTRACT Macaranga bancana is considered as a successful pioneer plant species. Usually found in disturbed and open areas, most of the current research focused on its relations with ants. One of the unique feature of the plants is that the seedling leaves are red, resembling and almost matching the background. Using a portable spectrometer, we measured the color reflectance of M. bancana seedlings (less than 20 cm in height). We also measured the leaf litter reflectance, adult M. bancana leaves and also seedlings of several other species found in the vicinity of M. bancana seedlings. The reflectances of M. bancana seedlings are very similar to that of the leaf litter background. We suggest that this cryptic coloration is crucial during the early stages of the plant when it still cannot rely on the protection of ants.

The flora composition of Sabang Island, Aceh, Indonesia

We provide a checklist of Angiosperms from a rapid flora inventory of Sabang Island, Aceh, Indonesia. This inventory, conducted in April 2010, was carried out to prepare a baseline data of the floral composition in the north-western island archipelago of Aceh. In this exercise, we covered a total of 7 plots (0.2 ha each) and 120 quadrats (1x1 m each) in 7 sampling locations within the island. In total, we recorded 325 species from 211 genera of 68 families. Nine predominant families (i.e. family with ≥ 10 species) are Euphorbiaceae (32 species), Cyperaceae (19 species), Fabaceae (17 species), Moraceae (15 species), Meliaceae (13 species), Lauraceae and Sterculiaceae (12 species, respectively), Rubiaceae (11 species) and Annonaceae (10 species). The volcanic soil of the island could have played a significant role in defining species composition and their abundance. On the non-woody species’ composition and abundance, the increasing magnitude of anthropogenic activities, for example, road and pathways constructions into the forested area, could have enhanced propagule dispersal of non-native plants into the area.

Measuring Orangutan nest structure using Unmanned Aerial Vehicle (UAV) and ImageJ

The nest is one of the crucial elements in orangutan daily activities. Previously, most of the nest structure studies were done manually by estimating measurement directly from visual observation. However, using the latest unmanned aerial vehicle (UAV) technology, we can reduce the workforce, time and energy while simultaneously ensuring the safety of the researcher conducting nest structure analysis. We recorded 49 pictures of orangutan nests at Sepilok Orangutan Rehabilitation Centre (SORC) using UAV (DJI Phantom 3 Quadcopter). The nest structure (length, depth, and width) was digitally measured by using ImageJ. Most of the nests were built at a strong, stable, and comfortable position at the top of the tree. Most orangutans chose Eusideroxylon zwageri to build nest compared to other tree species because of the strong and durable wood characteristic which would create a sturdy, strong and comfortable nest. We propose the use of drone with digital image analysis could provide a more accurate, less time consuming and safe method for studying orangutan nest structure.

Autofluorescence study and selected cyanidin quantification in the Jewel orchids Anoectochilus sp. and Ludisia discolor

Anoectochilus sp. and Ludisia discolor are known as Jewel orchids. Both species are terrestrial wild orchids that grow in shaded areas of forests. The Jewel orchids are renowned for the beauty of their leaves, which are dark-green laced with silvery or golden veins. The orchids are used as a cure in various parts of Asia. Overharvesting and anthropogenic disturbances threaten the existence of the Jewel orchids in the wild, necessitating human intervention in their survival. An understanding of the structure and adaptations of a plant may assist in its survival when propagated outside of its habitat. In this study, ex vitro leaves of Anoectochilus sp. and L. discolor were subjected to freehand sectioning, and then inspected through brightfield and fluorescence microscopy. The study indicated that all parts of both plants presented typical monocotyledonous characteristics except the leaves. The leaves displayed dorsiventrality with distinct palisade and spongy mesophyll layers. The spongy mesophyll layer contained cells which fluoresced a bright red when exposed to ultraviolet, blue, and green light wavelengths, hinting at the presence of anthocyanins for photoprotection. Cyanidin was detected in the leaves of L. discolor, as enumerated through high performance liquid chromatography (HPLC). The observations indicated that Anoectochilus sp. and L. discolor are well-adapted to live under shaded conditions with minimal exposure to light.