Raja Khanal

Candidate Gene Identification with SNP Marker-Based Fine Mapping of Anthracnose Resistance Gene Co-4 in Common Bean

Anthracnose, caused by Colletotrichum lindemuthianum, is an important fungal disease of common bean (Phaseolus vulgaris). Alleles at the Co–4 locus confer resistance to a number of races of C. lindemuthianum. A population of 94 F4:5 recombinant inbred lines of a cross between resistant black bean genotype B09197 and susceptible navy bean cultivar Nautica was used to identify markers associated with resistance in bean chromosome 8 (Pv08) where Co–4 is localized. Three SCAR markers with known linkage to Co–4 and a panel of single nucleotide markers were used for genotyping. A refined physical region on Pv08 with significant association with anthracnose resistance identified by markers was used in BLAST searches with the genomic sequence of common bean accession G19833. Thirty two unique annotated candidate genes were identified that spanned a physical region of 936.46 kb. A majority of the annotated genes identified had functional similarity to leucine rich repeats/receptor like kinase domains. Three annotated genes had similarity to 1, 3-β-glucanase domains. There were sequence similarities between some of the annotated genes found in the study and the genes associated with phosphoinositide-specific phosphilipases C associated with Co-x and the COK–4 loci found in previous studies. It is possible that the Co–4 locus is structured as a group of genes with functional domains dominated by protein tyrosine kinase along with leucine rich repeats/nucleotide binding site, phosphilipases C as well as β-glucanases.

Dynasty kidney bean

Abstract Dynasty is a dark red kidney bean (Phaseolus vulgaris L.) cultivar with excellent yield potential and resistant to races 17 and 73 of anthracnose.

AAC Shock navy dry bean

Abstract: AAC Shock is a high-yielding navy bean (Phaseolus vulgaris L.) cultivar with a semi-upright, indeterminate bush growth habit, large seed size, and resistance to common bacterial blight and races 1 and 15 of bean common mosaic virus. AAC Shock is recommended for dry bean growing areas in Ontario, Canada.

OAC Spark common bean

Abstract: OAC Spark is a determinate, early maturing navy bean (Phaseolus vulgaris L.) cultivar with an upright plant architecture and high yield potential. OAC Spark is adapted to and recommended for the dry bean growing areas in the Prairie provinces of Canada.

Mist common bean

Abstract: Mist is an indeterminate navy bean (Phaseolus vulgaris L.) variety with full season maturity, high yield potential, and resistance to common bacterial blight caused by Xanthomonas axonopodis pv. phaseoli. Mist is adapted and recommended for bean growing areas in southwestern Ontario.

Lighthouse common bean

Abstract: Lighthouse is an indeterminate, full-season navy bean (Phaseolus vulgaris L.) cultivar with an upright plant architecture, suitable for direct harvest, with a high yield potential, and a high level of resistance to common bacterial blight (CBB; caused by Xanthomonas axonopodis pv. phaseoli). Lighthouse is adapted to and recommended for the dry bean growing areas in southwestern Ontario.

Yeti kidney bean

Abstract Yeti is a full-season white kidney bean (Phaseolus vulgaris L.) cultivar with tall bush plant architecture; high yield potential andgood cooking quality. Yeti is adapted to and recommended for the dry bean growing areas in Southwestern Ontario.

Bolt common bean

Abstract The breeding line ACUG10-1 named as Bolt (CFIA registration no. 7366) of common bean (Phaseolus vulgaris L.) is resistant to anthracnose [caused by Colletotrichum lindemuthianum (Sacc. & Magnus)] with an upright plant architecture, suitable for direct harvest and high yield potential. Bolt is recommended for the dry bean growing areas in southwestern Ontario.

Fathom common bean

Abstract Fathom (CFIA registration no. 7544) is a full-season navy bean (Phaseolus vulgaris L.) cultivar with a high yield potential, and resistance to anthracnose (caused by Colletotrichum lindemuthianum ) and common bacterial blight (CBB; caused by Xanthomonas axonopodis pv. phaseoli). Fathom is recommended for the dry bean growing areas in southwestern Ontario.