Rajinder Raina

Sub-acute deltamethrin and fluoride toxicity induced hepatic oxidative stress and biochemical alterations in rats.

The current study investigated the effects of deltamethrin, fluoride (F−) and their combination on the hepatic oxidative stress and consequent alterations in blood biochemical markers of hepatic damage in rats. Significant hepatic oxidative stress and hepatic damage were observed in the toxicant exposed groups. These changes were higher in the deltamethrin-F− co-exposure treatment group, depicting a positive interaction between the two chemicals.

Haematolohical profile of subacute oral toxicity of molybdenum and ameliorative efficacy of copper salt in goats.

Molybdenum toxicity produces a state of secondary hypocuprosis, resulting into alterations in normal hematological profile. In the present study, ammonium molybdate alone and with copper sulfate (II) pentahydrate (ameliorative agent) was administered orally for 30 consecutive days in healthy goats of group 1 and 2, respectively, to access the effect on the hematological profile on different predetermined days of dosing. Administration of ammonium molybdate alone produced significant decline in the mean values of hemoglobin (Hb), packed cell volume (PCV), total leukocyte count (TLC), total erythrocyte count (TEC), and mean corpuscular hemoglobin concentration (MCHC), with a significant increase in neutrophil level and mean corpuscular volume (MCV). However, values of erythrocyte sedimentation rate, mean corpuscular hemoglobin, and differential leukocyte count were not significantly altered. On comparing observations of ameliorative group with the group 1 goats, it is concluded that the ameliorative copper salt has beneficial effects in alleviating the alterations in the values of Hb, PCV, TLC, TEC, MCV, MCHC, and neutrophils.

Effect of repeated oral administration of bifenthrin antioxidant status and acetylcholinesterase activity in brain of rats

The oxidative-stress-inducing potential of the pyrethroid, bifenthrin, in the brain of rats was evaluated following daily oral administration of 5.8 mg/kg body weight. Lipid peroxidation in the brain was increased, while the activities of superoxide dismutase, catalase, glutathione peroxidase, and glutathione S-transferase decreased. The activity of acetylcholinesterase (AChE) in erythrocytes and brain also decreased. Bifenthrin induces severe oxidative stress in brain, and inhibits brain and erythrocyte AChE.

Single and multiple daily dose toxicokinetics of fluoride after oral administration of sodium fluoride in goats

The toxicokinetics of fluoride was investigated in healthy cross breed goats after single and repeated oral administration of sodium fluoride (20 mg kg−1 b.wt.) for 30 days. Serial blood samples at various predetermined time intervals were collected for plasma separation on days 1 and 30 of administration. The plasma fluoride levels were estimated by using ion selective electrode of Orion Fluoride Analyzer using 96-09 combination fluoride electrode. The plasma levels of fluoride against time were adequately described by one compartment open model. The plasma peak level of fluoride was observed for 2 h. The toxicokinetic data revealed that for last dose, the mean values of β and ClB were significantly (p < 0.01) lower, whereas, the values of t 1/2β, AUC and MRT were significantly (p < 0.05) higher from their respective first dose values. The time concentration profile of plasma fluoride levels and toxicokinetics data revealed that repeated oral administration of fluoride has a cumulative effect in the body of the goats.