Rameez Raja Kaleri

Prevalence and Incidence of Staphylococcus aureus from Wound of Different Animal Species

The prevalence incidence and biochemical characteristics of Staphylococcus aureus isolated from wounds of buffalos, goats, dogs, donkeys and chickens were studied during present indigestion.The highest infection of Staphylococcus aureus was found in wound samples of buffalos (70.00%). as compared to goat, (33%), dog, (3%) donkey (40%) and chicken, (46.66%) respectively. The overall pure samples with Staphylococcus auerus from the animals was recorded as 39.13% while mixed infection was observed as 34.78%. The shape of Staphylococcus auerus isolated from buffalos, goat, and chicken were cocci, spherical, round in shape and characterized as G+ve. The Staphylococcus auerus isolated from all the animals were non-motile. It is concluded that highest infections of Staphylococcus aureus was found in buffalo (70.00%), whereas highest number of Staphylococcus aureus bacterial specie was observed as compared to other bacterial species. Prevalence and Incidence of Staphylococcus aureus from Wound of Different Animal Species

Influence of Phytohormones on Callus Indication and Micrpropagation on Rose (Rosa indica L.)

Roses ( Rosa indica L.) is one of the greatest significant flower collection in the biosphere and have commercial value in decorative, therapeutic and cosmetic trade. Speedy development and construction of disease free plants through in vitro technique have played a dynamic part in broadcast of salable rose cultivars. The five diverse meditations of growth controller such as (MS + 2.00 mg L -1 ), (IBA + 2.00 mg L -1 ), (AA + 30 gL -1 sucrose), (MS + 2.00 mg L -1 ), (BAP + 3.00 mg L -1 ), (NAA +30 mg L -1 sucrose), (MS + 2.00 mg L -1 ), (BAP + 3.00 mg L -1 ), ( IAA + 30 mg L -1 sucrose), (MS + 4 mg L -1 ), (BAP + 2.00 mg L -1 ), (( IAA + 30 mg L -1 sucrose) and (MS + 3.00 mg L -1 ), (BAP + 3.00 mg L -1 ), (IAA + 30 mg L -1 sucrose for shoot induction. After establishment of plantlets, stem was cast off as explants foundation for the initiation of callus. It is concluded that the two different concentrations 2.00 and 4.00 mg L -1 of 2 to 4 D were better worked out for callus induction.

Effect of Dextrose Sugar on the Growth and Production of Oyster Mushroom (Pleurotus ostreatus) through Tissue Culture

The study was conducted to investigate the dextrose sugar effect as carbon source on mycelial growth and production of Oyster mushroom ( Pleurotus ostreatus ). The experiment was performed in Mushroom Laboratory, Plant Pathology Section, Agriculture Research Institute, Tandojam, during 2013-2014. Mycelial growth was developed by using tissue culture on medium (PDA) potato dextrose agar with various concentrations of dextrose sugar. Analysis of variance for concentrations was statistically highly significant for all the parameters. In some cases among the different concentrations, 2.0% dextrose sugar showed after 2 days of micro propagation, the mycelial growth (1.9 cm) was recorded, followed by 1.5% dextrose sugar that showed (1.7 cm). The earlier spawn mycelia growth was observed in case of amending same 0/2% dextrose sugar (24.5 days). The pinhead first appeared (29.5 days) after the date of spawning by using 2.0% dextrose sugar. The minimum period (4.2 days) for maturation of mushroom fruiting body were recorded at 20% and 1.5% dextrose sugar. The maximum numbers of fruiting bodies (56.2) were observed with an application of dextrose sugar at 2.0%. The maximum number of bunches per bag (16.5) were harvested with an application of dextrose sugar 2.0%. The highest (350.5 g) fresh yield of Oyster mushroom Pleurotus ostreatus was recorded from 2.0% am ended of dextrose sugar.

Comparison of Stapler, Single Layer and Double Layer Techniques for Colon Closure in Dogs

The present study was performed on 18 healthy dogs (aged 16.80±1.22 months) and body weight (17.07± 2.21 kg) to determine the best suturing technique among single layer, double layer and stapler technique for the closure of colon in dogs. All dog were divided into three groups, placing 6 animals in each group, i.e. group A was closed with single layer suture technique, group B was closed with double layer suture technique and group C was closed with stapler technique. The number of stitches required for colon closure were 8.83, 16.33 and 9.16 in groups –A, B and C respectively. The mean number of stitches and time taken for the completion of double layer technique was significantly higher (P<0.01) than single layer and stapler techniques. The mean of total duration of abdominal closure was 47.00, 45.16 and 34.83 minutes in group A, B and C respectively. The mean of total duration for surgery was 57.16, 64.50 and 46.33 minutes in group A, B and C respectively..The study reveals that all the techniques were not completely free from complications and advantages. Although stapler technique had some advantages like minimal adhesion between anastomotic line and other structures, higher bursting wall tension at operated site and less duration required for closure of anastomosis. It is concluded that stapler technique used in this study proved better than single layer and double layer suture techniques in dogs.

Heritability Estimates for Some Growth Traits of Dhatti Camel Breed in Tharparkar

The present study was performed on 18 healthy dogs (aged 16.80±1.22 months) and body weight (17.07± 2.21 kg) to determine the best suturing technique among single layer, double layer and stapler technique for the closure of colon in dogs. All dog were divided into three groups, placing 6 animals in each group, i.e. group A was closed with single layer suture technique, group B was closed with double layer suture technique and group C was closed with stapler technique. The number of stitches required for colon closure were 8.83, 16.33 and 9.16 in groups –A, B and C respectively. The mean number of stitches and time taken for the completion of double layer technique was significantly higher (P<0.01) than single layer and stapler techniques. The mean of total duration of abdominal closure was 47.00, 45.16 and 34.83 minutes in group A, B and C respectively. The mean of total duration for surgery was 57.16, 64.50 and 46.33 minutes in group A, B and C respectively..The study reveals that all the techniques were not completely free from complications and advantages. Although stapler technique had some advantages like minimal adhesion between anastomotic line and other structures, higher bursting wall tension at operated site and less duration required for closure of anastomosis. It is concluded that stapler technique used in this study proved better than single layer and double layer suture techniques in dogs.

Effect of Genetic Parameters on Some Growth Performance Traits of Harnai Sheep

Present study was performed to estimates the genetic parameters forsome growth performance traits of Harnai sheep. The data was recorded for the period of 2004-2013 from the Multi-purpose research centre Yetabad, District, Baluchistan. The performance traits including birth, yearling, weaning and fleece weight was recorded for the estimation of genetic parameters. There was no significant difference was observed parity and Ram wise among some growth performance traits of Harnai sheep. While the results for heritability, estimation for birth weight, yearling weight, weaning weight and fleece weight was observed low to medium for some growth performance traits of Harnai sheep. It is concluded that low heritable and correlative traits mainly affected by the management, nutritional and temporary environmental conditions, hence improvement can be achieved through the better selection.

Correlation Estimates between Carcass Traits of Nili Ravi and Kundhi Buffalo

Present study was designed to estimates the correlation between carcass traits of Nili Ravi and Kundhi buffalo. The data for carcass traits of Nili Ravi and Kundhi buffalo was collected from Seven Star International Meat Processing Company Dhabeji at Thatta. In current study the data of total 100 animals of Kundhi and Nili Ravi breed were selected and divided into A, B, C and D group. In group A and C there were Kundhi and Nili Ravi male whereas, B and D females of both breeds respectively. The data including live body weight, carcass weight, dressing percentage and boneless weight of both breeds Kundhi and Nili Ravi were collected for the estimation of correlation. The results for correlation estimates of different carcass traits indicated that the correlation estimation were found positive and high among Nili Rave breed as compared to Kundhi breed, which shows that an increase in one carcass trait would increase the other carcass traits. It was concluded that Nili Ravi carcass traits are better expressed and produces more beef than Kundhi, while Kundhi male is better in beef production than the Nili Ravi female whereas Kundhi female produces low carcass yield.

Heritability Estimates for some Performances Traits of Baluchi Sheep

Present study was carried out to estimates the genetic parameters of Baluchi sheep during the year 2015 at Bhagnari cattle Cum Baluchi Sheep Farm Usta Muhammad, Baluchistan. The recorded data including (lactation yield and lactation length) was collected for the period 2005 to 2014.The results of current study revealed that average milk yield and lactation length was found 95.1±11.122kg and 123.60±8.44days of Baluchi sheep. The results for heritability and correlation estimates for lactation yield and lactation length was observed 0.113, 0.126 and 0.26, respectively. There was positive and low heritability and correlation was worked out for lactation yield and lactation length. Due to low results heritability and correlation estimates of some performance traits of Baluchi sheep, it was concluded that improvement can be achieved by process of mass selection.

Analysis of Growth and Carcass Traits of Dumbi Sheep Breed Male Lambs Different Management Systems

Present research was performed on twelve male lambs of Dumbi sheep breed kept in 2 management systems at Faculty of Animal Husbandry and Veterinary Sciences, Department of Livestock Management, Sindh Agriculture University, Tandojam. Animals were divided into two different groups. A groups animal were kept in semi intensive with provision of open grazing and concentrates while, Bgroup animals were kept in intensive management system with provision of green fodder and concentrate. Study was performed till 8 weeks and lambs were observed weekly foraverage body weight and carcass characteristics of both groups were recorded. The results of current study showed that average body weight of group A was (8.33 kg)found significantly high (P>0.05) as compared to the group B (6.86 kg). Moreover carcass characteristics of Dumbi lamb was also observed higher in group A as compared group B. While during comparison of the economical values of both groups, it was observed that group A was found more economical than group B. It is concluded that semi-intensive management system was found better as compared to intensive management system.

Impact of Climate Change on Sugarcane and Wheat Crops in District Hyderabad Sindh, Pakistan

Present research was conducted to observe the effect of climatically changes on agricultural crops, especially focusing on major climatic variable changes such as (temperature and rainfall) on wheat and sugarcane productions. Therefore this study is attempt to examine the climate change impact on production of wheat and sugarcane crops in Hyderabad district to measure the fluctuations every month during last 12 years from 2002 to 2014. Thus the following objectives were studied. To examine climate change (temperature and rainfall) scenario in the study area. To observe climate change impact on sugarcane and wheat crops of Hyderabad district, and to see the effect of temperature on the growth performance of wheat and sugarcane crop since 2002 to 2014. Findings of the study shows positive impact on sugarcane and wheat crop. Moreover 1 o C temperature increases then wheat yield increases 30.04 kgs/acre. Similarly 1 o C increase temperature increases sugarcane yield rise by the amount of 450 kgs/acre respectively. Additional, to see the average growth rate from 2002 to 2014, where it reveals that the temperature growth rate was increased 0.6 0 C in April. While 1 o C increased in June which is highest growth rate, similarly in July and August were 0.5 0 C and 0.5 0 C was increased respectively, Kharif temperature having increasing trend. Moreover in Rabi season there is high fluctuation in February which was 0.4 o C. It Is concluded that in last the trend of temperature fluctuations from 2002 to 2014. in the Kharif season temperature in April, May, June, July, August and September were 2.4, 1.25, 6.1, 0.85, 2.75, 3.55, moreover the fluctuations of Rabi season in October, November, December, January, February, March were average 2, 1.42, 1, 2.3, 1.1, and 2.2 respectively.