Randall W. Velliquette

The production of red blood cell alloantibodies in mice transfused with blood from transgenic Fyb‐expressing mice

BACKGROUND: A murine model would be useful to identify which immune mechanisms could be manipulated to treat or prevent red blood cell (RBC) alloimmunization in patients who become sensitized to multiple or widely expressed antigens.

STAR: a novel high-prevalence antigen in the Scianna blood group system.

BACKGROUND:  More than 20 years ago, a proband was described whose red blood cells (RBCs) typed Sc:1,−2,3. His serum sample contained an immunoglobulin G alloantibody that reacted with all RBCs tested except his own, his brother’s, and those with the Sc:−1,−2 phenotype. Cloning of the SC gene allowed determination of the molecular basis associated with this novel high‐prevalence antigen.

RHCE*ceTI encodes partial c and partial e and is often in cis to RHD*DIVa

BACKGROUND: In the Rh blood group system, variant RhD and RhCE express several partial antigens. We investigated RH in samples with partial DIVa that demonstrated weak and variable reactivity with anti‐C.

Alleles of the LAN blood group system: molecular and serologic investigations

Anti‐Lan has been implicated in hemolytic transfusion reactions and hemolytic disease of the fetus and newborn. The LAN blood group system is encoded by ABCB6, whose gene product, ABCB6, belongs to the ATP‐binding cassette (ABC) efflux transporter superfamily. The purpose of this study was to characterize additional alleles by analyzing DNA from 14 (13 unrelated) subjects whose red blood cells were serologically defined as Lan−, Lan+w/−, or Lan+w.

Absence of DOMR, a new antigen in the Dombrock blood group system that weakens expression of Dob, Gya, Hy, Joa, and DOYA antigens

BACKGROUND: The Dombrock (Do) blood group system consists of six distinct antigens: Doa, Dob, Gya, Hy, Joa, and DOYA. Our finding of a pregnant patient whose red blood cells (RBCs) were Hy+ but whose serum contained an apparent alloanti‐Hy suggested the presence of a seventh antigen and prompted this study.

New antigen in the Dombrock blood group system, DOYA, ablates expression of Doa and weakens expression of Hy, Joa, and Gya antigens

BACKGROUND: The Dombrock (Do) blood group system consists of five distinct antigens: Doa, Dob, Gya, Hy, and Joa. Our finding of a patient whose plasma contained a Do‐related alloantibody suggested the presence of a sixth antigen.

Three new high-prevalence antigens in the Cromer blood group system

BACKGROUND: The Cromer blood group system consists of nine high‐prevalence and three low‐prevalence antigens carried on decay‐accelerating factor (DAF). This report describes three new Cromer high‐prevalence antigens, named ZENA, CROV, and CRAM.

RHCE*ceAG (254C>G, Ala85Gly) is prevalent in blacks, encodes a partial ce‐phenotype, and is associated with discordant RHD zygosity

RHCE*ceAG has the nucleotide change c.254C>G, which encodes p.Ala85Gly associated with altered expression of e antigen. We analyzed serologic and DNA‐based testing data on samples with RHCE*ceAG to determine its effect on antigen expression, linkage with RHD, and its prevalence in African Americans.

Murine monoclonal anti‐s and other anti‐glycophorin B antibodies resulting from immunizations with a GPB.s peptide

BACKGROUND: The blood group antigens S and s are defined by amino acids Met or Thr at position 29, respectively, on glycophorin B (GPB). Commercial anti‐s reagents are expensive to produce because of the scarcity of human anti‐s serum. Our aim was to develop hybridoma cell lines that secrete reagent‐grade anti‐s monoclonal antibodies (MoAbs) to supplement the supply of human anti‐s reagents.

Novel GYP(A-B-A) hybrid gene in a DANE+ person who made an antibody to a high-prevalence MNS antigen

BACKGROUND: The glycophorin (GP) molecule associated with the GP.Dane phenotype is a GP(A‐B‐A) hybrid that contains some amino acids encoded by the Pseudoexon 3 of GYPB and Asn45 of GPA and carries the low‐prevalence MNS antigens DANE and Mur. Serum from a woman of English ancestry contained an immunoglobulin M alloantibody to a high‐prevalence MNS antigen, and the purpose of this study was to identify the molecular basis of her phenotype.