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Featured researches published by Raphael Kudela.


SpringerPlus | 2013

Identification of harmful cyanobacteria in the Sacramento-San Joaquin Delta and Clear Lake, California by DNA barcoding

Tomofumi Kurobe; Dolores V. Baxa; Cécile E Mioni; Raphael Kudela; Thomas R Smythe; Scott Waller; Andrew D Chapman; Swee J. Teh

Accurate identification of cyanobacteria using traditional morphological taxonomy is challenging due to the magnitude of phenotypic plasticity among natural algal assemblages. In this study, molecular approach was utilized to facilitate the accurate identification of cyanobacteria in the Sacramento-San Joaquin Delta and in Clear Lake in Northern California where recurring blooms have been observed over the past decades. Algal samples were collected from both water bodies in 2011 and the samples containing diverse cyanobacteria as identified by morphological taxonomy were chosen for the molecular analysis. The 16S ribosomal RNA genes (16S rDNA) and the adjacent internal transcribed spacer (ITS) regions were amplified by PCR from the mixed algal samples using cyanobacteria generic primers. The obtained sequences were analyzed by similarity search (BLASTN) and phylogenetic analysis (16S rDNA) to differentiate species sharing significantly similar sequences. A total of 185 plasmid clones were obtained of which 77 were successfully identified to the species level: Aphanizomenon flos-aquae, Dolichospermum lemmermannii (taxonomic synonym: Anabaena lemmermannii), Limnoraphis robusta (taxonomic synonym: Lyngbya hieronymusii f. robusta) and Microcystis aeruginosa. To date, Dolichospermum and Limnoraphis found in Clear Lake have only been identified to the genus lavel by microscopy. During the course of this study, morphological identification and DNA barcoding confirmed A. flos-aquae as the predominant cyanobacterium in the Sacramento-San Joaquin Delta indicating a shift from M. aeruginosa that have dominated the blooms in the past decade. Lastly, the species-specific identification of Limnoraphis robusta in Clear Lake is another significant finding as this cyanobacterium has, thus far, only been reported in Lake Atitlan blooms in Guatemala.


Oceanography | 2008

New Insights into the Controls and Mechanisms of Plankton Productivity in Coastal Upwelling Waters of the Northern California Current System

Raphael Kudela; Neil S. Banas; John A. Barth; Elizabeth R. Frame; David A. Jay; John L. Largier; Evelyn J. Lessard; Tawnya D. Peterson; Andrea J. Vander Woude


Oceanography | 2017

GlobalHAB A New Program to Promote International Research, Observations, and Modeling of Harmful Algal Blooms in Aquatic Systems

Elisa Berdalet; Raphael Kudela; Ed Urban; Henrik Enevoldsen; Neil S. Banas; Eileen Bresnan; Michele Astrid Burford; Keith Davidson; Christopher J. Gobler; Bengt Karlson; Po Teen Lim; Lincoln MacKenzie; Marina Montresor; Vera L. Trainer; Gires Usup; Kedong Yin


Archive | 2017

A massive harmful algal bloom on the U.S. West Coast and the future of monitoring for early warning

Vera L. Trainer; Stephanie K. Moore; Ryan M. McCabe; Barbara M. Hickey; Raphael Kudela; Roman Marin; John Mickett; Christina M. Mikulski


Archive | 2017

Designing an observing system for early detection of harmful algal blooms

Bengt Karlson; C.R Andersson; Kathryn J. Coyne; Raphael Kudela; K.G. Sellner; Donald M. Anderson


Archive | 2015

From GEOHAB to GlobalHAB - International Research and Coordination of HABs Leading to Improved Societal Benefits

Raphael Kudela; Elisa Berdalet


Archive | 2015

SCOR-IOC Global Ecology and Oceanography of Harmful Algal Blooms (GEOHAB) Program & GlobalHAB : Progress Report 2014-2015 : Background document for Agenda item 4 and 5

Elisa Berdalet; Raphael Kudela


Archive | 2015

SCOR-IOC Global Ecology and Oceanography of Harmful Algal Blooms (GEOHAB) Program: Progress Report 2013-2015

Elisa Berdalet; Raphael Kudela


Archive | 2014

GEOHAB 2014. Global Ecology and Oceanography of Harmful Algal Blooms, GEOHAB Synthesis Open Science Meeting

Elisa Berdalet; Steward Bernard; Michele Astrid Burford; Henrik Enevoldsen; Raphael Kudela; Rob Magnien; Suzanne Roy; Patricia A. Tester; Ed Urban; Gires Usup


Harmful algae news | 2006

GEOHAB core research project - HABs in upwelling systems : Call for participants

Grant C. Pitcher; F. G. Figueiras; Raphael Kudela; Trevor A. Probyn; Teresa Moita; Sonia Sanchez; Vera L. Trainer; Ed Urban; Henrik Enevoldsen

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Elisa Berdalet

Spanish National Research Council

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Ed Urban

University of Delaware

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Vera L. Trainer

National Oceanic and Atmospheric Administration

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Neil S. Banas

University of Washington

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Gires Usup

National University of Malaysia

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F. G. Figueiras

Spanish National Research Council

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Alan Hastings

University of California

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