Rebecca West

Taming anxiety in laboratory mice

Routine laboratory animal handling has profound effects on their anxiety and stress responses, but little is known about the impact of handling method. We found that picking up mice by the tail induced aversion and high anxiety, whereas use of tunnels or open hand led to voluntary approach, low anxiety and acceptance of physical restraint. Using the latter methods, one can minimize a widespread source of anxiety in laboratory mice.

Monitoring for adaptive management in a trial reintroduction of the black-footed rock-wallaby Petrogale lateralis

Reintroduction practitioners must often make critical decisions about reintroduction protocols despite having little understanding of the reintroduction biology of the focal species. To enhance the available knowledge on the reintroduction biology of the warru, or black-footed rock-wallaby Petrogale lateralis MacDonnell Ranges race, we conducted a trial reintroduction of 16 captive individuals into a fenced predator and competitor exclosure on the Anangu Pitjantjatjara Yankunytjatjara Lands in South Australia. We conducted seven trapping sessions and used radio-tracking and camera traps to monitor survival, reproduction and recruitment to the population over 36 months. Blood samples were collected pre-release and during two trapping sessions post-release to assess nutritional health. The survival rate of founders was 63%, with all losses occurring within 10 weeks of release. Post-release blood biochemistry indicated that surviving warru adapted to their new environment and food sources. Female warru conceived within 6 months of release; 28 births were recorded during the study period and 52% of births successfully recruited to the population. Our results suggest that captive-bred warru are capable of establishing and persisting in the absence of introduced predators. However, the high mortality rate immediately post-release, with only a modest recruitment rate, suggests that future releases into areas where predators and competitors are present should use a trial approach to determine the viability of reintroduction. We recommend that future releases of warru into unfenced areas include an intensive monitoring period in the first 3 months post-release followed by a comprehensive long-term monitoring schedule to facilitate effective adaptive management.

Conservation challenges and benefits of multispecies reintroductions to a national park – a case study from New South Wales, Australia

Species reintroductions into predator-controlled areas are an increasingly used conservation tool. Typically, ecological outcomes of such projects (e.g. recruitment, predation) are the focus but seldom necessary legislative, policy, social and institutional processes required for establishing large projects. This particularly applies to protected areas, managed by governments for conservation. Reintroductions are recommended for a third of threatened Australian vertebrates, with the New South Wales Government boldly embarking on a 2013 initiative to return locally extinct mammals to three protected areas. We detail the legislative, policy, social and institutional processes required for one of these sites, Wild Deserts, in Sturt National Park. Seven locally extinct species, including the greater bilby (Macrotis lagotis), western barred bandicoot (Perameles bougainville), golden bandicoot (Isoodon auratus), greater stick-nest rat (Leporillus conditor), crest-tailed mulgara (Dasycercus cristicauda), western quoll (Dasyurus geoffroii) and burrowing bettong (Bettongia lesueur) will be reintroduced into two exclosures. The scale of the project required identification of an appropriate location, satisfaction of statutory requirements for major infrastructure in a national park, assessments of potential ecological costs and benefits, engagement of the national park agency and other stakeholders (Traditional Owners, other government agencies, neighbours), and staff training to meet government agency requirements. We outline the resourcing, costs and benefits of such a project on government-managed land along with lessons learnt for similar large-scale restoration and reintroduction projects. Future projects would benefit from understanding legislative and policy frameworks and the need for transparency, while maximising efficiencies.

Testing the potential for supplementary water to support the recovery and reintroduction of the black-footed rock-wallaby

Abstract Context. Supplementary resource provision is increasingly used by conservation managers to manipulate habitat conditions that limit population growth of threatened species. These methods are popular in reintroduction programs because they can assist released individuals to adapt to novel environments. In situ management and reintroductions are being used to recover warru (black-footed rock-wallaby, Petrogale lateralis MacDonnell Ranges race) on the arid Anangu Pitjantjatjara Yankunytjatjara (APY) Lands of South Australia. Direct predation by introduced predators is thought to be the main cause of population decline, but indirect predation effects reducing access to water resources has also been proposed as a limiting factor. Aims. To determine whether warru would use supplementary water and so provide a tool to alleviate resource pressure for in situ (wild) and reintroduced warru populations. Methods. We provided supplementary water to a wild and reintroduced warru population across 12 months. Drinking rates were calculated by monitoring water points with camera traps and modelled against plant moisture content and total rainfall. We also examined whether number of visits to water points by warru predators and competitors was significantly different to control points (no water present). Key results. Wild and reintroduced warru used water points within 0–10 days of installation. No significant increase in visits by predators or competitors was observed at water points. Drinking rates were significantly higher during dry winter months (March–October) for both wild and re-introduced populations. Conclusions. Supplementary water is readily utilised by warru. Water could be provided in this manner to warru populations where predators are present, particularly during drier months (generally March–October on the APY Lands), periods of drought or after fire, when food resources will have a lower water content and/or be less abundant. This may increase breeding rates and recruitment of young, and improve the probability of persistence for populations of this threatened species, and should be further investigated. Implications. Supplementary water provision may be a useful tool to increase population growth rates for threatened mammalian herbivores in arid habitats. Experimental trials of the uptake of supplementary water and effects on population dynamics will provide important data for implementing adaptive management frameworks for conservation.

More than just the animals: opportunities and costs of reintroducing threatened black-footed rock-wallabies to remote Indigenous land

Conservation activities, including translocations of threatened species, are increasingly important priorities for conserving biodiversity and culture on Indigenous-managed lands, which occupy over 20% of the Australian landmass. In the Anangu Pitjantjatjara Yankunytjatjara (APY) Lands of north-western South Australia, the Warru Recovery Team reintroduced the threatened black-footed rock-wallaby (warru) to Wamitjara in May 2018. We outline the unique planning and logistical issues inherent in securing support and involvement of Traditional Owners and Indigenous rangers for predator, weed and fire management, cross-fostering, captive breeding, monitoring and translocation of the rock-wallabies. Altogether, the project cost AU

Searching for an effective pre-release screening tool for translocations: can trap temperament predict behaviour and survival in the wild?

3.86 million (actual – AU

Deep evolutionary experience explains mammalian responses to predators

1.86 million, in kind – AU

Predator exposure improves anti‐predator responses in a threatened mammal

2.00 million) over 10 years of preparation and one year of post-translocation monitoring. This consisted of AU

Looking back to go forward: genetics informs future management of captive and reintroduced populations of the black-footed rock-wallaby Petrogale lateralis

1.18 million for cross-fostering and captive breeding at Monarto Zoo and a further AU

Designer prey: Can controlled predation accelerate selection for anti-predator traits in naïve populations?

0.55 million for the construction and maintenance of a fenced exclosure in the APY Lands. It is estimated that AU