Reginaldo Nassar Ferreira

Quantificação das Bactérias Sólido-Aderidas, Bactérias e Protozoários Líquido-Associados do Rúmen de Bovinos Jovens Alimentados com Amiréia

ABSTRACTS - It were utilized four crossbreed, castrated, rumen cannuladed bovines, receiving dietary starea (containing 30% ofurea) in the concentrate and corn silage, with the objective of quantify the liquid-associated bacteria and protozoa, solid-associatedbacteria and related them with the ruminal pH. Three samples from ruminal content were harvested at 1 a.m., 2 a.m. and 30 min a nd 11a.m. and 30 min after feeding. The microbial mass was quantified and qualified in the different fractions of solid-associated b acteria (SAB),liquid-associated bacteria (LAB) and liquid-associated protozoa (LAP) and their nitrogen contents (N), dry matter (DM) and orga nicmatter (OM) were determined. The microbial population presented increasing contribution of SAB with the time, what did not occu rwith LAB and LAP. The level of OM in DM from the SAB increased as well, until 2 a.m. and 30 min when remains unaffected until11 a.m. and 30 min. The nitrogen levels of the SAB expressed in the organic matter decreased in equal or superior time of harve st at2 a.m. and 30 min, although the N level in the dry matter did not present this difference. The relations between bacteria:proto zoa wereequal to 1: 2.1 at 1 hour, 2.6: 1 at 2h and 30 min and 2.2: 1 at 11h and 30 min after the feeding. It was still observed after feeding prevalenceof protozoa and bacteria, both associated to the ruminal liquid. The total amounts and the ruminal microbial pool were not infl uencedby pH, probably because it always maintained itself above 6.39.Key Words: dry matter, organic matter, nitrogen, ruminal microorganisms

Anti-Inflammatory, Antinociceptive, and Sedating Effects of Lafoensia pacari. Aqueous Extract

Abstract The acetic acid–induced abdominal writhing, the tail flick, and the croton oil–induced mouse ear edema tests were used to study the anti-inflammatory effects of the aqueous extract from Lafoensia pacari. A. St.-Hil. (Lythraceae) stem bark. Pentobarbital-induced sleeping time was used to study the central nervous system depressant effect of the extract. The aqueous extract caused a dose-dependent inhibition of acetic acid–induced abdominal writhing and ear edema formation and produced a significant (p < 0.05) dose-related increase in the duration of sleep. The results suggest that Lafoensia pacari. stem bark contains compounds with anti-inflammatory, analgesic, and central depressant actions.

Central activities of hydroalcoholic extract from Lafoensia pacari A. St.-Hil. stem bark

Lafoensia pacari A. St.-Hil. can be found from Amapa to Rio Grande do Sul states, and also in Paraguay and Bolivia. It is popularly known as pacari or mangava-brava and is used to promote weight loss, as an anti-thermal or tonic, to treat gastritis, ulcers, scarring, itching, discouragement, and cancer. In the open field tests, the hydroalcoholic extract from L. pacari stem bark (HEP) decreased the number of rearings, number of invaded squares, and increased immobility time compared to control animals. In the pentobarbital-induced sleep time test, HEP decreased latency time to sleep and increased sleeping time. In the rota-rod test, no changes in the studied parameters were observed. In the elevated plus maze, HEP increased the percentage time and percentage entries in the open arms, indicating that this extract exerts an anxiolytic-like activity.

Animal performance and carcass characteristics from confined lambs fed on concentrate feed and additives

The number of sheep flocks in Brazil is increasing. It is known that lambs must be slaughtered when young for producing quality meat. The current study evaluated the inclusion of protected methionine, protected lysine, lysophospholipid and amylolytic enzymes in a diet to lambs and their effects on weight gain and quantitative carcass traits at slaughtering. Eighty non-castrated male crossbred Dorper x Santa Inês lambs, 20.57 ± 4.33 kg live weight, were used. The feedlot lasted 64 days and 60 animals were slaughtered. There were no differences for live weight, daily feed intake, feed conversion and average daily weight gain at the first 28 days of feedlot. From the 28th day lysophospholipid treatment presented the highest live weight. Lysophospholipid and amylolytic enzyme presented the best performance in average daily gain, followed by protected methionine, control and protected lysine. Lysophospholipid treatment presented higher daily feed intake rates than protected lysine and protected methionine. Feed conversion was lower for amylolytic enzyme and higher for control. No changing in carcass traits was reported due to additives. Better performance may be achieved with feedlot lambs fed on diets with the addition of amylolytic enzyme and lysophospholipid at the finishing phase.