Renata Antunes Esteves

Effects of Rosmarinus officinalis essential oil on germ tube formation by Candida albicans isolated from denture wearers

INTRODUCTION The aim of this study was to investigate the effects of Rosmarinus officinalis essential oil on germ tube formation by Candida albicans isolated from denture wearers. METHODS Ten C. albicans isolates recovered from denture wearers were tested using 10% fetal bovine serum with or without 4% R. officinalis essential oil. RESULTS The essential oil from R. officinalis completely inhibited germ tube formation in the investigated C. albicans isolates. CONCLUSIONS The results demonstrate that the essential oil of R. officinalis modulates C. albicans pathogenicity through its primary virulence factor (i.e., germ tube formation was suppressed).

Antifungal activity of Rosmarinus officinalis Linn. essential oil against Candida albicans, Candida dubliniensis, Candida parapsilosis and Candida krusei

La actividad de formacion del tubo antifungico y anti-germen del aceite esencial de Rosmarinus officinalis Linn. fue verificada en cuatro cepas de Candida (C. albicans, C. dubliniensis, C. parapsilosis y C. krusei). Se obtuvieron halos de inhibicion y concentracion inhibitoria minima (MIC) utilizando las pruebas de difusion radial y de microdilucion, respectivamente. La concentracion fungicida minima (MFC) se obtuvo a traves de ensayo de la MIC. Ademas, se evaluo el efecto del aceite esencial en la formacion del tubo germinativo de C. albicans y C. dubliniensi. La MIC50 vario de 0,5% a 2%, mientras que la MFC vario de 1% a 2%. Se observo la inhibicion total del crecimiento del tubo germinativo en C. albicans y C. dubliniensis. El aceite esencial de R. officinalis Linn demostro una potente actividad inhibitoria y fungicida contra cepas especificas de Candida

Isolation of Candida spp. from denture-related stomatitis in Pará, Brazil

The aim of this study was to isolate and identify Candida species from the oral cavity of denture wearers with denture-related stomatitis who were attended at the University Federal of Pará (Belém City, Pará State, Brazil). A total of 36 denture wearers with denture-related stomatitis were included, and type I (50%), type II (33%) and type III (17%) stomatitis were observed. Candida spp. were isolated from 89% of the cases and included five different Candida species. C. albicans was the most frequently recovered species (78% of the cases), followed by C. famata and C. tropicalis. We observed a significant association between Candida species isolation and unsatisfactory denture condition (p = 0.0017). Our results demonstrated the highly frequency of Candida species isolation in denture wearers with denture-related stomatitis and showed the relationship between these species and poor denture maintenance.

The effect of Rosmarinus officinalis essential oil on germ tube formation in Candida dubliniensis recovered from denture users

Candida dubliniensis is an emerging pathogenic yeast that is closely related to C. albicans and colonizes or infects the oral cavities of denture users. Similar to C. albicans, C. dubliniensis produces germ tubes as a major virulence factor. Due to the scarcity of data demonstrating that natural products have anti-C. dubliniensis activity, the objective of this study was to evaluate the effect of an essential oil from Rosmarinus officinalis on C. dubliniensis germ tube formation. Six isolates of C. dubliniensis were recovered from the oral cavities (n = 5/denture base and n = 1/palatal mucosa) of six denture users either with (n = 4) or without (n = 2) stomatitis. Germ tube formation was induced in Sabouraud dextrose broth containing 10% fetal bovine serum in the presence (test) or absence (control) of 4% R. officinalis essential oil. The complete inhibition of germ tube formation for all of the tested isolates of C. dubliniensis was observed (p = 0.004). Our results demonstrate the potent anti-C. dubliniensis effect of R. officinalis essential oil, indicating the need to further investigate the pharmaceutical use of R. officinalis essential oil, particularly as it impacts the colonization of denture resin and stomatitis.