Riccardo Fortina

Fatty Acid Composition of the Seed Oils of Selected Vicia L. Taxa from Tunisia

Whole mature seeds of eight selected varieties, subspecies and accessions of three Vicia L. species grown in Tunisia were investigated for their fatty acid (FA) profile. The FA composition ranged from lauric (C12:0) to lignoceric (C24:0) acids. The total FA content was 1235.14 to 1580.34 mg 100 g–1 dry matter (DM). Linoleic acid (C18:2 c 9c 12; 647.87 to 801.93 mg 100 g–1 DM, i.e. >50% of total FA), oleic acid (C18:1 c 9; 181.32 to 346.79 mg 100 g–1 DM, i.e. 13.2 to 24.6% of total FA) and α-linolenic acid (C18:3 c 9c 12c 15; 42.01 to 97.72 mg 100 g–1 DM, i.e. 3.4 to 7.1% of total FA) were the most abundant unsaturated FA. Palmitic acid (C16:0; 189.86 to 281.07 mg 100 g–1 DM, i.e. 15.4 to 17.8% of total FA) and stearic acid (C18:0; 24.35 to 52.75 mg 100 g–1 DM, i.e. 2.0 to 4.0% of total FA) were the major saturated ones. The sum of all other FA did not exceed 3.0% of TFA. The favourable FA profile of the studied vetch seeds makes them interesting cheap diet components to be used in the nutrition of ruminants and non-ruminants reared in the dryland agricultural regions of Mediterranean countries.

The establishment of a database of Italian feeds for the Cornell Net Carbohydrate and Protein System

Abstract A field application of the Cornell Net Carbohydrate and Protein System (CNCPS) in Italy has been limited because the feed bank is based on North American feedstuffs and still few laboratories are able to analyze feeds as requested by the CNCPS. Moreover, the standardization of analytical procedures is still not homogeneous among laboratories. This work was carried out to establish a first database for feeds commonly used in Italy, providing nutritionists and producers an accurate and current feed composition, also indicating methods and apparatus for analytical procedures potentially available for routine analysis. A total of 909 samples of hays, silages and raw materials (protein feeds, cereals and by-products) were analyzed through 1999 and 2002; analysis included protein solubility and degradability, protein fractions, structural carbohydrate fractions and the calculation of neutral detergent structural carbohydrates. When possible, average data were compared with those included in the feed bank of CNCPS ver. 3 and with those obtained by another Italian laboratory. The main differences were observed in chemical composition of forages and silages, whose composition largely depends on environmental conditions and physiological stage; protein feeds, cereals and by-products showed some differences in crude protein, soluble protein and protein fractions even in feeds of national origin. The intent to modify the feed bank values of CNCPS for establishing an Italian data base of feeds will require a collaborative study of many laboratories not only for forages, hays and silages samples - whose composition is greatly dependent on environmental factors and agronomic techniques - but also for protein fractions, whose values are largely influenced by even small changes in analytical techniques.

Evaluation of equine oocyte developmental competence using polarized light microscopy

The purpose of this study was to observe in vitro-matured equine oocytes with an objective computerized technique that involves the use of a polarized light microscope (PLM) in addition to the subjective morphological evaluation obtained using a classic light microscope (LM). Equine cumulus-oocyte complexes (COCs, n = 922) were subjected to different in vitro maturation times (24, 36 or 45 h), however, only 36-h matured oocytes were analyzed using CLM. The 36-h matured oocytes that reached maturity were parthenogenetically activated to evaluate the quality and meiotic competence. Average maturation percentages per session in groups 1, 2 and 3 (24-, 36- and 45-h matured oocytes respectively) were 29.31 ± 13.85, 47.01 ± 9.90 and 36.62 ± 5.28%, whereas the average percentages of immature oocytes per session were 28.78 ± 20.17, 7.83 ± 5.51 and 22.36 ± 8.39% respectively. The zona pellucida (ZP) birefringent properties were estimated and correlated with activation outcome. ZP thickness and retardance of the inner layer of the zona pellucida (IL-ZP) were significantly increased in immature oocytes compared with mature oocytes (P < 0.001 and P < 0.01 respectively). The comparison between parthenogenetically activated and non-activated oocytes showed a significant increase in the area and thickness of the IL-ZP in parthenogenetically activated oocytes (P < 0.01). These results show that the 36-h in vitro maturation (IVM) protocol allowed equine oocytes to reach maturity, and PLM observation of ZP can be used to distinguish mature and immature oocytes as well as activated and non-activated oocytes.

Typical edible non-dairy animal products in Africa from local animal resources

Abstract This review aims to identify the main typical non-dairy edible products of animal origin available in Africa, describing their production processes and their strengths and constraints. Farm animals are mainly raised in an extensive, family-run system; there is, however, a significant development of intensive poultry production. Meat products are usually obtained by drying, but meat and/or offal can also be stored as stuffed products and can be additionally treated by smoking and/or curing. The increasing poultry production provides eggs and meat at low price. The small-scale/family farms are managed mainly by women and children, with a positive social impact. The assets and limits of local breeds and of extensive versus semi-extensive or intensive production systems are discussed. Seafood are an essential source of proteins, minerals and micronutrients. Due its high perishability, the proportion of cured fish in this continent is higher than the world average. Wildlife can supply high-quality meat, but attention must be paid to the vulnerable/endangered species and to the sanitary aspects of this food chain. Insects are traditionally consumed in Africa, supplying very cheap highly nutritive food, with low environmental impact. Finally, a variety of honey and other bee products, including some Slow Food praesidia, are described. From the point of view of the respect of biodiversity and ecosystems, local culture, accessibility and nutritional requirements, animal productions in Africa are usually carried out in a sustainable way; however, the low efficiency of most traditional production systems represents an important limit, also in relation to export opportunities.

Typical dairy products in Africa from local animal resources

Abstract Milk is recognised as an important element of the pastoral civilisations in Africa, where it has not only an economic value, but also a social and cultural value. The objective of this review is to identify the main African dairy products, mainly focussing on typical production processes based on the use of local animal resources. To this aim, dairy products were classified into five categories, according to the type and the production method: fresh cheese, ripened cheese, fermented milk, butter and dairy by-products. These categories give rise to an extraordinary range of local products where tradition, culture, and environmental conditions and constraints play a major role in contributing to this diversification. In some countries, some attempts are being made in order to valorise the local dairy products by establishing Slow Food Presidia or including them in the Ark of Taste compiled by Slow Food. One of the main constraints that limits the commercialisation and export of African dairy products is related to the scarce hygiene in milk production and processing, and the frequent absence of starter cultures, which result in products with non-standardised characteristics, short shelf life and high spoilage susceptibility, with consequent risks for human health. Efforts should be made to support the dairy products chain in Africa, by making production processes safer and by valuing local resources, in order to expand the internal market and the export opportunities. Development cooperation projects may play an important role in this respect and should therefore be encouraged.

Effect of ruminally unprotected Echium oil on milk yield, composition and fatty acid profile in mid-lactation goats

This study investigated the effects on goat milk yield and composition of a diet supplemented with Echium plantagineum oil (EPO). Twenty-four mid-lactation multiparous Camosciata goats were divided into two balanced groups and fed for 44 d a diet based on hay and concentrate, supplemented (EPO group, Echium) or not (CON group, control) with 40 ml of ruminally unprotected EPO. Individual milk yield was recorded and individual milk samples were collected at 11, 22, 33, and 44 d after supplementation. Milk samples were analysed for milk components and fatty acids (FA). Data were statistically analysed by repeated-measures analysis of variance. Milk yield, protein and lactose contents were significantly higher in EPO than CON group. The inclusion of EPO significantly decreased total saturated FA and total branched-chain FA, and contemporarily sharply increased trans biohydrogenation intermediates (P ⩽ 0.001). Milk concentration of α-linolenic, stearidonic and γ-linolenic acids increased by 23, 1000 and 67%, respectively (P ⩽ 0.001). Due to extensive ruminal biohydrogenation, their apparent transfer rate was less than 3%. As a consequence, the milk concentrations of very long-chain (VLC) polyunsaturated fatty acids (PUFA), such as eicosapentaenoic (20:5 n-3) and dihomo-γ-linolenic (20:3 n-6) acids, significantly increased with EPO treatment, but values remained very low. Docosahexaenoic acid (22:6 n-3) was undetectable in all analysed milk samples. Results show that ruminally unprotected EPO can enhance milk yield and protein and improve the overall goat milk FA profile. However, this kind of supplementation cannot be considered a valuable strategy to develop goat functional dairy products enriched with VLC n-3 PUFA for human consumption.

Nutritive Value and Energy Content of the Straw of Selected Vicia L. Taxa from Tunisia

The chemical composition and energy value of straws of Vicia sativa L. (varieties Languedoc and Mghila, and subspecies amphicarpa) and Vicia villosa Roth. (variety Sejenane and accession 2565) were investigated. The plants were grown in a Mediterranean environment, under identical environmental conditions. Total digestible nutrients (TDN) and energy values (digestible energy, metabolisable energy, and net energy for lactation) were estimated according to the equations developed by the 2001 Dairy National Research Council. Both ether extract (EE) or total fatty acids (FA) amounts, and digestible neutral detergent fibre (dNDF) calculated from chemical analysis or measured using a 48-hour rumen in vitro assay were used for calculations. Significant differences were observed in the chemical composition and energy value of the straws of the considered V. sativa and V. villosa varieties. Within the same variety, the TDN was similar using either EE or FA values for calculation. The energy resulted largely dependent on the dNDF values. Energy was higher when in vitro dNDF was used for calculation in low-NDF straw samples, while the opposite occurred for high-NDF samples.

Effect of an automatic feeding system on growth performance and feeding behaviour of pigs reared outdoors

Abstract Nine Mora Romagnola and 10 Large White x Mora Romagnola growing pigs were reared outdoors. In both groups ad libitum feed was provided. Conventional pigs received it twice a day, distributed in two long troughs. Inside the corral of the second group, an automatic station was set up for: feed distribution, pigs weighing, and control by an analog camera. Thus the self-feeders received feed ad libitum individually by the automatic system, divided into small quantities at meal times. During the experiment the analog camera was used over 24 hours each day, to collect pictures of pigs in order to investigate their behaviours. For each picture the day and hour, the number of visible pigs and their behaviours were recorded and a statistical analysis of data, which was expressed as hourly frequencies of behavioural elements, was performed. Moreover to highlight “active” and “passive” behaviours between the groups, two categories “Move” and “Rest” were created grouping some behavioural elements. With regard to performance, conventional pigs reached a higher total weight gain (56.1±2.42 kg vs 46.7±2.42 kg; P=0.0117). But the feed conversion index (FCI) of both groups was similar. The self-feeders had consumed less feed than conventional animals. The feeding system seems to influence behaviours. The percentage of time spent in Eating activity differs (P<0.0001) between the self-fed (median 24.6%) and conventional pigs (median 10.9%). The resulting more regular eating trend of self-feeders influenced the daily activities distribution. The behavioural category Rest (median: self-feeders 55.0% vs 71.4% conventional pigs) was dominant, with conventional pigs becoming more restless, particularly at meal times. This type of feeding competition and aggressive behaviour did not happen in the self-feeders due to the feed distribution system. The self-feeder results showed that pigs eat at the automatic station both day and night. The animals perform on average 3 visits per hour at night and 10 during the day, with an average duration of some minutes (from 3 to 5 approximately).