Richard C. Russell

Bed Bugs: Clinical Relevance and Control Options

SUMMARY Since the late 1990s, bed bugs of the species Cimex lectularius and Cimex hemipterus have undergone a worldwide resurgence. These bed bugs are blood-sucking insects that readily bite humans. Cutaneous reactions may occur and can start out as small macular lesions that can develop into distinctive wheals of around 5 cm in diameter, which are accompanied by intense itching. Occasionally, bullous eruptions may result. If bed bugs are numerous, the patient can present with widespread urticaria or eythematous rashes. Often, bites occur in lines along the limbs. Over 40 pathogens have been detected in bed bugs, but there is no definitive evidence that they transmit any disease-causing organisms to humans. Anemia may result when bed bugs are numerous, and their allergens can trigger asthmatic reactions. The misuse of chemicals and other technologies for controlling bed bugs has the potential to have a deleterious impact on human health, while the insect itself can be the cause of significant psychological trauma. The control of bed bugs is challenging and should encompass a multidisciplinary approach utilizing nonchemical means of control and the judicious use of insecticides. For accommodation providers, risk management procedures should be implemented to reduce the potential of bed bug infestations.

MOSQUITO-BORNE ARBOVIRUSES IN AUSTRALIA : THE CURRENT SCENE AND IMPLICATIONS OF CLIMATE CHANGE FOR HUMAN HEALTH

Of the mosquito-borne arboviruses, the encephalitic Murray Valley encephalitis and Kunjin viruses are a major public health concern, but the arthritides Ross River and Barmah Forest viruses are more important in a public health sense, being responsible for a far greater number of infections. Reported cases of Ross River totalled approximately 30,000 during 1991-1996; there have been several widely separated outbreaks of Barmah Forest in recent years and case reports are increasing annually. Surveillance programmes have increased our understanding of the geographic regions, climatic conditions and vector factors associated with viruses. Virus activity is widespread but is often localised, is driven primarily by mosquito abundance and various species are involved; host factors are involved also, but are not well understood. Typically, mosquito populations are governed by availability of habitat and environmental conditions. Models of climate change predict increases in rainfall, tides and temperature for parts of Australia, and such changes have the potential to increase the risk of arbovirus transmission by increasing the distribution and abundance of vectors, and duration of mosquito and arbovirus seasons. However, the amplitude of climate change is uncertain and the ecology of arbovirus transmission is complex. It is likely that some areas will have increases in arbovirus activity and human infection with predicted climate change, but risk of increased transmission will vary with locality, vector, host and human factors.

Arboviruses associated with human disease in Australia.

Mosquito-borne arboviruses are an important public health issue in Australia. The alphaviruses Ross River and Barmah Forest virus are widespread and active annually, and cause debilitating polyarthritis. The flaviviruses Murray Valley encephalitis, Kunjin and Japanese encephalitis virus are restricted in distribution and activity but may cause life-threatening illness, and dengue viruses are active in some areas.

FIELD EFFICACY OF THE BG-SENTINEL COMPARED WITH CDC BACKPACK ASPIRATORS AND CO2-BAITED EVS TRAPS FOR COLLECTION OF ADULT AEDES AEGYPTI IN CAIRNS, QUEENSLAND, AUSTRALIA

ABSTRACT In this study, we compared the efficacy of the newly available BG-Sentinel with an established “gold standard,” the CDC Backpack Aspirator, and a CO2-baited EVS trap for the collection of Aedes aegypti (L.) in Cairns, Australia. BG-Sentinels collected significantly more (P = 0.017) female Ae. aegypti (mean per collection, 1.92 ± 0.39) than both the CDC Backpack Aspirator (1.00 ± 0.35) and the EVS trap (0.71 ± 0.27). Male-only and combined male-female Ae. aegypti collections for the BG-Sentinel and the CDC Backpack Aspirator were also greater than EVS trap collections. The CDC Backpack Aspirator and the BG-Sentinel captured proportionally fewer females compared with the EVS trap. The BG-Sentinel was the most Ae. aegypti-specific collection method. The CDC Backpack Aspirator collected proportionally more bloodfed Ae. aegypti than the other methods, which collected a greater proportion of nullipars. The data presented here will aid researchers in deciding what Ae. aegypti sampling device best suits their needs. BG-Sentinels and CDC Backpack Aspirators should be considered as alternatives to human-bait collections for Ae. aegypti sampling.

Mark–release–recapture study to measure dispersal of the mosquito Aedes aegypti in Cairns, Queensland, Australia

Abstract.  In Queensland, Australia, in response to isolated cases of dengue infection, larval control of the vector Aedes aegypti (L.) (Diptera: Culicidae) is targeted at breeding sites within 200 m of a case and interior spraying with a pyrethroid adulticide is targeted at premises within 100 m. To ascertain whether these limits are appropriate, we conducted a mark–release–recapture study to measure the dispersal of female Ae. aegypti in the city of Cairns where transmission occurs. Female mosquitoes reared from wild collected eggs were differentially marked with fluorescent dust depending on whether they were to be released blood‐fed or non‐blood‐fed, and a total of 1948 females was released. A total of 132 sticky ovitraps was set at 64 premises within a 200 m radius and collections of trapped adults were made at 5–15 days post‐release. Sixty‐seven females (3.4%) were recaptured, with the furthest being caught 200 m from the release point, and the mean distance travelled was 78 m. Overall, 23.1% of the recaptures outside the release site were taken beyond 100 m by day 15. Dispersal was comparable for both blood‐fed and non‐blood‐fed releases. There was a significant tendency for dispersal to be in a north‐westerly direction, probably because of the presence of numerous containers and heavy shading by trees in this direction and a busy road to the south of the release point that appeared to inhibit dispersal. The results suggest that adulticiding may have to be extended beyond 100 m if more than 8 days have elapsed since female Ae. aegypti could have fed upon a viraemic dengue case. The study also shows that dispersal is not random, and that it may be possible to maximize vector control by taking into account environmental factors that affect the direction of female mosquito flight.

Constructed wetlands and mosquitoes: Health hazards and management options—An Australian perspective

Abstract Constructed wetlands are increasingly being installed to ‘polish’ urban drainage and storm water by reducing contaminants before disposal into river systems. Unfortunately, they also provide habitat for mosquitoes that can be nuisance pests and transmit pathogens such as arboviruses and malaria. In Australia, Ross River virus is responsible for thousands of cases annually of a disease that is severely debilitating, has regional incidence rates often exceeding 1:1000, and costs millions of dollars in health and other impacts. Disease transmission depends on mosquito species and abundance, and extent of contact with humans; the characteristics and siting of wetlands determine hazards, and indicate risks for nuisance or disease. Mosquito control should not rely solely on chemical and biological agents. Design of wetlands is important: shallow water and dense vegetation promote mosquito production. Deeper habitats with cleaner steeper margins, and more open water, produce fewer mosquitoes. Water and vegetation management can reduce mosquitoes: aeration and sprinkler systems, and flooding and drainage regimes, can reduce larval densities; vegetation thinning can assist mosquito predators. Such measures may appear incompatible with objectives and operations of wetlands, but mosquito management must be an integral objective of modern wetland design and maintenance in order to minimise health hazards.

Characterization of Virulent West Nile Virus Kunjin Strain, Australia, 2011

An encephalitis outbreak among horses was caused by a pathogenic variant of Kunjin virus.

Discovery of a Widespread Infestation of Aedes albopictus in the Torres Strait, Australia

ABSTRACT Aedes albopictus is a container-breeding Stegomyia mosquito that has dispersed widely from its origins in Southeast Asia. Because Ae. albopictus is a known dengue vector and a potential vector of a variety of arboviruses and it can tolerate cooler climates than Aedes aegypti, Australian quarantine and health authorities have strategies to detect and eliminate it from international ports. Following the detection of 42 adult Ae. albopictus in BG-Sentinel traps set on Yorke island in the Torres Strait of Australia in April 2005, extensive surveys were conducted to determine the distribution of Ae. albopictus in the Torres Strait and adjoining Cape York Peninsula. A total of 17 islands and the northern peninsula area of Cape York Peninsula were surveyed by collection of larvae and pupae from flooded containers and human bait collections of adult mosquitoes with aspirators and sweep nets. Aedes albopictus was detected on 10 islands and comprised 100% of the day-biting container-breeding mosquitoes on Yorke and Stephens Islands. No Ae. albopictus were detected in the mainland sites on Cape York. Retrospective genetic analysis of larvae collected in April 2004 and April 2005 on Yorke Island indicated that Ae. albopictus was present in low densities in 2004 and that there were 3 genetically distinct mitochondrial haplotypes on Yorke Island in April 2005. Additionally, on Yorke Island there is evidence that Ae. albopictus is displacing Aedes scutellaris.

Enhanced arbovirus surveillance with deep sequencing: Identification of novel rhabdoviruses and bunyaviruses in Australian mosquitoes

Viral metagenomics characterizes known and identifies unknown viruses based on sequence similarities to any previously sequenced viral genomes. A metagenomics approach was used to identify virus sequences in Australian mosquitoes causing cytopathic effects in inoculated mammalian cell cultures. Sequence comparisons revealed strains of Liao Ning virus (Reovirus, Seadornavirus), previously detected only in China, livestock-infecting Stretch Lagoon virus (Reovirus, Orbivirus), two novel dimarhabdoviruses, named Beaumont and North Creek viruses, and two novel orthobunyaviruses, named Murrumbidgee and Salt Ash viruses. The novel virus proteomes diverged by ≥ 50% relative to their closest previously genetically characterized viral relatives. Deep sequencing also generated genomes of Warrego and Wallal viruses, orbiviruses linked to kangaroo blindness, whose genomes had not been fully characterized. This study highlights viral metagenomics in concert with traditional arbovirus surveillance to characterize known and new arboviruses in field-collected mosquitoes. Follow-up epidemiological studies are required to determine whether the novel viruses infect humans.

A lethal ovitrap-based mass trapping scheme for dengue control in Australia: II. Impact on populations of the mosquito Aedes aegypti

In Cairns, Australia, the impacts on Aedes aegypti L. (Diptera: Culicidae) populations of two types of ‘lure & kill’ (L&K) lethal ovitraps (LOs), the standard lethal ovitrap (SLO) and the biodegradable lethal ovitrap (BLO) were measured during three mass‐trapping interventions. To assess the efficacy of the SLO, two interventions (one dry season and one wet season) were conducted in three discrete areas, each lasting 4 weeks, with the following treatments: (i) SLOs (>200 traps, ∼4/premise), BG‐sentinel traps (BGSs; ∼15, 1/premise) and larval control (container reduction and methoprene treatment) and (ii) larval control alone, and (iii) untreated control. Female Ae. aegypti populations were monitored for 4 weeks pre‐ and post‐treatment in all three areas using BGSs and sticky ovitraps (SOs) or non‐lethal regular ovitraps (ROs). In the dry season, 206 SLOs and 15 BGSs set at 54 and 15 houses, respectively, caught and killed an estimated 419 and 73 female Ae. aegypti, respectively. No significant decrease in collection size of female Ae. aegypti could be attributed to the treatments. In the wet season, 243 SLOs and 15 BGSs killed ∼993 and 119 female Ae. aegypti, respectively. The mean number of female Ae. aegypti collected after 4 weeks with SOs and BGSs was significantly less than the control (LSD post‐hoc test). The third mass‐trapping intervention was conducted using the BLO during the wet season in Cairns. For this trial, three treatment areas were each provided with BLOs (>500, ∼4/premise) plus larval control, and an untreated control area was designated. Adult female Ae. aegypti were collected for 4 weeks pre‐ and post‐treatment using 15 BGSs and 20 SOs. During this period, 53.2% of BLOs contained a total of 6654 Ae. aegypti eggs. Over the intervention period, collections of Ae. aegypti in the treatment areas were significantly less than in the control area for BGSs but not SOs. An influx of relatively large numbers of young females may have confounded the measurement of changes in populations of older females in these studies. This is an important issue, with implications for assessing delayed action control measures, such as LOs and parasites/pathogens that aim to change mosquito age structure. Finally, the high public acceptability of SLOs and BLOs, coupled with significant impacts on female Ae. aegypti populations in two of the three interventions reported here, suggest that mass trapping with SLOs and BLOs can be an effective component of a dengue control strategy.