Robert J. Salmon

Effects of x-irradiation on phospholipid metabolism. I. Investigation of P32 uptake into individual liver and spleen phosphatides of normal and irradiated mice.

Phospholipids, tagged with P32, of liver and spleen of normal and irradiated mice were separated by silicic acid column chromatography. The speciiic radioactivity of the total phospholipids and of seven groups of phosphatides was determined at various times after the injection of the P32 in both normal and irradiated animals. The uptake of P32 into the total liver phospholipids was higher in the irradiated animals than in the controls. This was due to increased specific activity of the cephalins and lecithins. The specific activity of inositides and lysolecithin was not greatly changed by irradiation, whereas that of the sphingomyelins was increased at 2 and 4 hours but decreased after 8 hours. The specific radioactivity of liver phospholipids reached a maximum at 8 hours after injection of the P32 in normal animals but occurred at 6 hours in the irradiated animals.

Effect of X-Radiation on Uptake of P32 into Individual Nucleotides of HeLa Cells

Summary Separation of 24 acid soluble phosphorus compounds from irradiated and non-irradiated HeLa cells is described. Relative specific activities of 19 of these compounds are given. Irradiation of the cells produced an increase of relative specific activity and amount of uridine diphosphoacetylglucosamine, and a decrease in amount and specific activity of adenosine triphosphate.