Robert M. Dowben

The biosynthesis of radioactive estradiol I. synthesis by surviving tissue slices and cell-free homogenates of dog ovary

1. 1. 2-14C-acetate is incorporated into estradiol, estrone and cholesterol by surviving tissue slices and homogenates of dog ovary. 2. 2. Under comparable conditions more acetate was incorporated into estradiol than into estrone. 3. 3. The activity of estradiol and estrone obtained from tissues of animals in proestrus was greater than that of animals in anestrus.

On the hydrolysis and oxidation of glucuronolactone.

Abstract 1. 1.The non-enzymic hydrolysis of glucuronolactone has been studied in distilled water and Tris and phosphate buffer. It proceeds at a faster rate in phosphate than in Tris buffer. 2. 2.Enzymic hydrolysis of glucuronolactone can be demonstrated in liver homogenates while kidney shows little or no activity. In liver, enzymic activity is present in the high speed supernatant primarily. 3. 3.Using kidney homogenates with Tris buffer, it is found that glucuronolactone is more readily oxidized to carbon dioxide than glucuronate.

Effects of Hexahydrocoenzyme Q4 on Murine Muscular Dystrophy

Summary The administration of hexahydrocoenzyme Q4 (20 μg/g of body weight) resulted in an increase in muscle strength of dystrophic mice with a hanging time on a vertical screen of 44.5 sec after 4 weeks of treatment compared to 5.5 sec for untreated controls. A slight decrease in the efflux rate of potassium accompanied treatment, but there was no change in the electrolyte composition of dystrophic muscle.