Roger G. Lentle

FEED PARTICLE SIZE: IMPLICATIONS ON THE DIGESTION AND PERFORMANCE OF POULTRY

This review highlights the limited amount of research conducted regarding the optimum particle size of different feeds for efficient poultry production. The current industry practice of using highly processed, pelleted diets masks the influence of particle size, but some reports suggest that the effects of feed particle size on performance may be maintained even after pelleting. There appears to be a general consensus that particle sizes of broiler diets based on maize or sorghum, optimum particle size should be between 600 and 900 μm. Available data clearly show that grain particle size is more critical in mash diets than in pelleted or crumble diets. Although it has been postulated that finer grinding increases substrate availability for enzymatic digestion, there is evidence that coarser grinding to a more uniform particle size improves the performance of birds maintained on mash diets. This counter-intuitive effect may result from the positive effect of feed particle size on gizzard development. A more developed gizzard is associated with increased grinding activity, resulting in increased gut motility and greater digestion of nutrients. Although grinding to fine particle size is thought to improve pellet quality, it will markedly increase energy consumption during milling. Systematic investigations on the relationships of feed particle size and diet uniformity with bird performance, gut health and pellet quality are warranted if efficiency is to be optimised in respect of the energy expenditure of grinding.

Physical characteristics of digesta and their influence on flow and mixing in the mammalian intestine: a review

The physical properties of digesta may influence mixing, efficiency of digestion, and absorption within the lumen of the intestine. We review how the physical properties of digesta change during transit through the various segments of the intestine, and how their influence on flow and mixing may be modulated by peristaltic activity. We examine how, in more fluid digesta, the solid and liquid phases interact to influence flow and mixing. Similarly, how in viscid digesta, shear strength, plasticity and elasticity of contained particulate material may influence the permeation of the fluid phase and secretions into and out of the digesta bolus. The manner in which the solid and liquid phases of digesta interact in a partly gaseous environment, such as the lower bowel, to influence bolus cohesion is also examined. Those mechanisms that promote the formation of a less viscous layer at the mucosal interface to promote plug flow are reviewed, and their effect on the efficiency of mixing and digestion discussed. It is recommended that in any future work investigating the character of mixing in the intestine, a wider range of appropriate digesta properties be measured and that, in investigations of intestinal movement, perfusates with similar characteristics to digesta be used.

Influence of Feed Particle Size on the Performance, Energy Utilization, Digestive Tract Development, and Digesta Parameters of Broiler Starters Fed Wheat- and Corn-Based Diets

The influence of particle size and grain type on the performance, AME(n), and on gross morphological and histological parameters of the various segments of the digestive tract of broilers fed wheat- or corn-based diets was investigated. The experimental design was a 2 x 2 factorial arrangement of treatments evaluating 2 particle sizes (fine and coarse) and 2 grain types (wheat and corn). The 2 particle sizes were achieved by grinding the whole grains in a hammer mill to pass through 1-mm and 7-mm screen sizes. Broiler starter diets, based on wheat- or corn-soybean meal, were formulated, pelleted, and each diet was offered to 6 cages of 8 male broilers each from d 1 to 21 posthatching. The results showed that the differences in particle size distribution still existed between diets after pelleting especially in the proportion of coarse particles (1 mm and over). In corn-based diets, coarse grinding improved (P = 0.06) weight gains compared with fine grinding, but this particle size effect was not observed in wheat-based diets. In both diets, coarse grinding lowered (P < 0.001) feed per gain of broilers compared with fine grinding. In wheat-based diets, coarse grinding improved (P = 0.06) AME(n) compared with fine grinding. Heavier (P < 0.05) gizzard weights were observed in birds fed the coarse corn-based diet, but particle size had no effect on the gizzard size in birds fed wheat-based diets. Villus height, crypt depth, and epithelial thickness in the duodenum were unaffected (P > 0.05) by particle size and grain type. Overall, the present results showed that the effect of feed particle size varies depending on grain type.

Influence of insoluble fibre and whole wheat inclusion on the performance, digestive tract development and ileal microbiota profile of broiler chickens

1. An experiment of 21-d duration was conducted to examine the effects of diluting wheat-based diets with insoluble fibre sources and whole wheat inclusion on the performance, nutrient utilisation, digestive tract development and ileal microbiota profile of broiler chickens. The treatments were as follows: Treatment 1, control diet based on ground wheat; Treatment 2, where 200 g/kg whole wheat replaced the ground wheat pre-pelleting; and Treatments 3 and 4 where the control diet was diluted with fine cellulose and wood shavings, respectively, at a ratio of 6 : 100 (w/w). 2. Weight gains and apparent metabolisable energy were unaffected by dietary treatment. Gain : feed ratio was not influenced by the inclusion of whole wheat or wood shavings, but decreased with cellulose inclusion. However, when gain:feed of birds was corrected by subtracting the amount of cellulose and wood shavings from the total feed consumption, it was found that the inclusion of wood shavings increased gain : feed, while cellulose inclusion had no effect. Similarly, AMEN was unaffected by dietary treatment. However, when AMEN was corrected for energy contribution from cellulose or wood shavings, improvements in AMEN were observed in these two treatments. 3. Wood shavings increased the relative gizzard weights and improved ileal starch digestibility compared to other dietary treatments. All gut components were shorter in birds given diets containing cellulose and wood shavings compared to those receiving the control and whole wheat diets. 4. Ileal microbiota profiling, using denaturing gradient gel electrophoresis, showed that microbial composition was affected by dietary treatment and that the treatments were grouped into two main clusters. The two groupings showed similarity between birds receiving the control and cellulose diets and similarity between birds fed on the whole wheat and wood shavings diets. 5. The findings suggest that the effects of insoluble fibre on broiler performance differed depending on the fibre particle size.

The physical processes of digestion

Introduction Flow mixing and absorption. The physical problems of extracting nutrients from food items and the possible macroscopic and microscopic solutions. The digestive tube and its limitations. solid to liquid to solid Methods for evaluating the physical properties of digesta Particle size Rheology Viscometry Viscoelastic behaviour Time dependent behaviour Permeametry Hindered settling function Permeability and other measures Methods for evaluating the relationship between motility and flow of digesta Solid and liquid phase markers Reactor mixing Spatiotemporal mapping Physical behaviour of fluid digesta Macroscopic effects Newtonian and non Newtonian behaviour of fluids. Where digesta fit in this system the kinetics of digesta flow Securing efficient absorption from fluids and non-Newtonian fluids, chemical reactor theory and problems Buoyancy Backflow form coiled elements Physical behaviour of solid digesta The continuum between solids and liquids viscoselasticity securing efficient absorption from fluids and from viscoelastic fluids Permeability extrusion of the liquid phase Propulsion and mixing of digesta the interplay between the gut wall and its contents Tension and stretch receptors in the enteric nervous system Maintaining the flow of digesta problems of narrowing and expanding Co-evolution of motility and the physical properties of digesta Micromixing Diffusion. Mucus and the unstirred water layer. Flow in the paravillus space and the crypts. Tight junctions permeability and fluid flow Glycocalyceal signalling of shear The physics of food What is known about how the physical structure of food interacts with the digestive processes e.g starch granule digestion digestion of proteinaceous aggregates eetc ( Dr Allan Hardacre NZ Crop and Food ) Flow and microorganisms Adaptions of micro-organisms to move within digesta and mucus Glycocalyceal signalling Messing with the properties of digesta Adulterating foods with viscoactive substances Nutraceuticals planning the rate of nutrient release. Modulating lumen pressure Microencapsulation and adherence to the gut wall

High-definition spatiotemporal mapping of contractile activity in the isolated proximal colon of the rabbit

Four types of contractile activity were identified and characterised in the isolated triple haustrated proximal colon of the rabbit using high-definition spatiotemporal mapping techniques. Mass peristalses were hexamethonium-sensitive deep circular contractions with associated taenial longitudinal contractile activity that occurred irregularly and propagated rapidly aborad, preceded by a zone of local lumen distension. They were sufficiently sustained for each event to occupy the length of the isolated colonic segment and the contraction persisted longer orally than aborally, the difference being more pronounced when lumen contents were viscous. Haustra were bounded by deep even-spaced ring contractions that progressed slowly aborad (haustral progression). Haustral formation and progression were hexamethonium-sensitive and coordinated across intertaenial domains. Ripples were hexamethonium-resistant phasic circular contractions that propagated predominantly orad at varying rates. In the presence of haustra, they were uncoordinated across intertaenial domains but were more coordinated when haustra were absent. Fast phasic contractions were relatively shallow hexamethonium-resistant contractions that propagated rapidly in a predominantly aborad direction. Fast phasic circular contractions were accompanied by taenial longitudinal muscle contractions which increased in amplitude prior to a mass peristaltic event and following the administration of hexamethonium. On the basis of the concurrence and interaction of these contractile activities, we hypothesise that dual pacemakers are present with fast phasic contractions being modulated by the interstitial cells of Cajal in the Auerbach’s plexus (ICC-MY) while ripples are due to the submucosal ICC (ICC-SM). Further, that ICC-SM mediate the enteric motor neurons that generate haustral progression, while the intramuscular ICC (ICC-IM) mediate mass peristalsis. The orad movement of watery fluid was possibly due to ripples in the absence of haustra.

Periodic fluid extrusion and models of digesta mixing in the intestine of a herbivore, the common brushtail possum (Trichosurus vulpecula)

The digesta in four gut compartments (proximal and distal halves of small intestine, caecum, and proximal colon) of a wild hindgut fermenting herbivore, the common brushtail possum (Trichosurus vulpecula), were investigated by rheometry and permeametry. Digesta from all compartments were highly viscous and exhibited shear-thinning. Apparent viscosity was positively related to dry matter content, and increased from proximal small intestine to colon. Dynamic rheological measurements showed that in small intestinal digesta the elastic modulus was greater than the viscous modulus and their ratios were characteristic of weak gels, indicating that digesta could sustain compression. The apparent viscosity of distal small intestinal digesta was markedly lower when measured by capillary viscometry than by rotatory viscometry, indicating that plug flow was likely to be facilitated by lubrication from a peripheral layer of less viscous fluid; i.e., there was an augmented plug flow. Permeametry showed that fluid was extruded from all digesta on compression at physiological pressures, that there was significant permeability of proximal and distal small intestinal digesta, but that digesta became progressively compacted during permeation, with a concomitant reduction in permeability as dry matter content increased. It is proposed that conditions within the small intestine differ from those of an ideal plug flow reactor as radial mixing and turbulence cannot occur. Instead, we suggest that segmentation and peristalsis aid radial mixing of the fluid phase by compressing the solid phase, with extrusion of fluid through the digesta plug. This extrusion may be followed by resorption of fluid back into the plug when the elasticity of the solid phase of digesta is Hookean, thus aiding the mixing of secreted enzymes with insoluble substrates within the plug.

Manipulating digestion with foods designed to change the physical characteristics of digesta.

We explore how foods can be designed to modulate digestion and to promote health by changing the physical properties of digesta. The physical characteristics of digesta are discussed along with their impact on the physiology of digestion with special reference to sites where these characteristics are likely to influence digestive efficiency. Evidence is reviewed regarding the effects of supplementation with viscoactive agents on the flow and mixing of digesta in particular segments of the human gut that, by changing the rheology and liquid permeability of digesta in that segment, influence specific aspects of digestion and absorption.

The bioelectrical basis and validity of gastrointestinal extracellular slow wave recordings

•  Extracellular recording techniques are commonly used to measure bioelectrical activity. However, the validity of gastrointestinal extracellular recordings has recently been challenged. •  In this joint experimental and modelling study, slow waves were recorded during contractile inhibition, biphasic and monophasic slow wave potentials were recorded simultaneously, and the biophysical basis of extracellular potentials was modelled with comparison to experimental data. •  The results showed that in vivo extracellular techniques reliably recorded slow waves in the absence of contractions, and potentials recorded using conventional serosal electrodes (biphasic) were concordant in phase and morphology with those recorded using suction electrodes (monophasic). •  Modelling further demonstrated that the morphology of experimental recordings is consistent with the biophysics underlying slow wave depolarisation. •  In total, these results demonstrate that gastrointestinal extracellular recordings are valid when performed and analysed correctly, reliably representing bioelectrical slow wave events. Motion suppression is not routinely required for in vivo extracellular studies.

Quantification of the effects of the volume and viscosity of gastric contents on antral and fundic activity in the rat stomach maintained ex vivo.

AimsThe aim of this study was to examine the effect of varying the rheological properties of perfusate on the volume and muscular activity of the various compartments of the rat stomach.MethodsImage analysis was used to quantify the activity of the ex vivo stomach preparations when perfused according to a ramp profile.ResultsThe area of the fundus increased to a greater extent than that of the body when watery or viscous material was perfused. However, initial distension of the corpus was greater and occurred more rapidly when viscous material was perfused. Only the fundus expanded when perfusion followed the administration of verapamil. The frequency of antrocorporal contractions decreased significantly and the amplitude of antrocorporal contractions increased significantly with increase in gastric volume. The velocity of antrocorporal contractions did not vary with gastric volume but varied regionally in some preparations being faster distally than proximally. Neither the frequency, amplitude or velocity of antrocorporal contractions differed when pseudoplastic rather than watery fluid was perfused. However, the characteristics of antrocorporal contractions changed significantly when the stomach was perfused with material with rheological characteristics that induce different patterns of wall tension to those normally encountered. Hence, the mean frequency and speed of propagation of antrocorporal contractions increased and their direction of propagation became inconstant.