Rogério Anderson Marcasso

Meningiomas em cães: aspectos clínicos, histopatológicos e imuno-histoquímicos

Neoplasias of the central nervous system (CNS) of small animals are frequently diagnosed; however, data relative to prevalence are scarce. The aim of this study was to evaluate retrospectively the occurrence of primary CNS tumors in dogs in a Veterinary Teaching Hospital and describe clinical, histopathological and immunohistochemical aspects of the most common tumors. Fourteen cases of CNS primary neoplasia (prevalence of 0.27%) were identified from 1998 to 2013; from these 11 were diagnosed as meningiomas. The mean age of dogs with meningiomas was 10 years; male (7/11) and the Boxer breed (3/11) were most commonly represented. Seven meningiomas were located in the spinal cord and four intracranially; the main clinical signs were impaired locomotion and seizures, respectively. Pulmonary metastasis was found in two cases. Myelography was performed in six dogs with spinal meningiomas. In these cases it was effective in demonstrating the deviation or interruption of the contrast column. Exploratory surgery to confirm diagnosis or remove the tumor was performed in five animals and the survival rate varies 85 to 960 days. Meningiomas were histologically classified as transitional (4/11), meningothelial (2/11), papillary (2/11), angiomatous (1/11), microcystic (1/11) and anaplastic (1/11). Evaluation of histological grade was performed; 10 meningiomas were classified as grade I and one as grade III. The neoplastic cells of eight tumors (8/11) were positive for Massons trichrome and one with the Congo red histochemical techniques. The immunohistochemical assays revealed in all tumors a positive immunoreactivity for vimentin but negative staining for factor VIII and p53. Immunolabelling for S100 (6/11), GFAP (5/11) and pancytokeratin (3/11) showed a variable staining intensity. The mean cell proliferation index was 3.2 mitotic figures and 3.4% for Ki-67 immunostaining. The results confirmed that meningiomas are the most frequent primary CNS neoplasia in dogs, with variation in histological subtypes. Histochemical and immunohistochemical characterization contributed to the diagnosis; however, studies of the expression of other genes are necessary to assist with the prognosis of this kind of neoplasia.

Molecular survey of infectious agents associated with bovine respiratory disease in a beef cattle feedlot in southern Brazil

We investigated the occurrence of infectious pathogens during an outbreak of bovine respiratory disease (BRD) in a beef cattle feedlot in southern Brazil that has a high risk of developing BRD. Nasopharyngeal swabs were randomly collected from steers (n = 23) and assessed for the presence of infectious agents of BRD by PCR and/or RT-PCR assays. These included: Histophilus somni, Mannheimia haemolytica, Pasteurella multocida, Mycoplasma bovis, bovine respiratory syncytial virus (BRSV), bovine coronavirus (BCoV), bovine viral diarrhea virus (BVDV), bovine alphaherpesvirus 1 (BoHV-1), and bovine parainfluenza virus 3 (BPIV-3). Pulmonary sections of one steer that died with clinical BRD were submitted for pathology and molecular testing. The frequencies of the pathogens identified from the nasopharyngeal swabs were: H. somni 39% (9 of 23), BRSV 35% (8 of 23), BCoV 22% (5 of 23), and M. haemolytica 13% (3 of 23). PCR or RT-PCR assays did not identify P. multocida, M. bovis, BoHV-1, BVDV, or BPIV-3 from the nasopharyngeal swabs. Single and concomitant associations of infectious agents of BRD were identified. Fibrinous bronchopneumonia was diagnosed in one steer that died; samples were positive for H. somni and M. haemolytica by PCR. H. somni, BRSV, and BCoV are important disease pathogens of BRD in feedlot cattle in Brazil, but H. somni and BCoV are probably under-reported.

Bovine pulmonary lesions found in post-mortem inspection in slaughterhouse in state Paraná

Polyana Carolina Marino, Gercio Luiz Bonesi, Luiz Carlos Negri Filho, Daniella Furlan, Débora Nayara Augusto, Alexey Leon Gomel Bogado, Rogério Anderson Marcasso, Luiz Cesar da Silva, Marcelo Diniz dos Santos, Werner Okano 1 Docente. Departamento de Clínica Médica e Cirúrgica de Grandes Animais. UNINGA, Maringá, PR. pollyveterinaria@gmail.com 2 Docente. Medicina Veterinária. UNOPAR. Arapongas, PR. gercio.bonesi@agricultura.gov.br; rogeriomarcasso@gmail.com 3 Mestrando do Programa de Pós Graduação em Saúde de Produção de Ruminantes. PROSUP/CAPES. UNOPAR. Arapongas, PR. luiz.negri@hotmail.com; furlandanie@hotmail.com; debora_augusto@outlook.com 4 Docentes do Programa de Pós Graduação em Saúde de Produção de Ruminantes. UNOPAR. Arapongas, PR. alexey_leon@hotmail.com; silvaluizcesar@gmail.com; werner.okano@unopar.br 5 Docente do Programa de Pós Graduação em Biociência Animal. UNIC. Cuiabá, MT. marcelo.diniz@kroton.com.br __________________________________________________________________________________

Congenital Mature Intracranial Teratoma in a Pampas Deer ( Ozotoceros bezoarticus ) in Brazil.

Abstract We describe a congenital mature intracranial teratoma in a Pampas deer (Ozotoceros bezoarticus) in southern Brazil. We found an irregular, spongy, space-occupying mass in the brain. The tumor consisted of well-differentiated tissues that derived from all three germ layers.