Luiz Carlos Negri Filho

Association of Histophilus somni with spontaneous abortions in dairy cattle herds from Brazil

This study investigated the participation of infectious agents in spontaneous abortions and reproductive problems at eight dairy cattle herds from three geographical regions of Brazil. Fourteen aborted fetuses and the organ sections of one cow with history of repeated abortions were received for pathological evaluations and molecular diagnostics. PCR/RT-PCR assays targeted specific genes of abortifacient agents of cattle: bovine viral diarrhea virus (BVDV), bovine herpesvirus 1 (BoHV-1), Listeria monocytogenes, Neospora caninum, Leptospira spp., Brucella abortus, and Histophilus somni. Six fetuses were adequate for pathological investigations; one of these did not demonstrate remarkable pathological alterations. Significant histopathological findings included vasculitis, hemorrhage, and fibrinous thrombosis of the cerebrum (n = 4); necrotizing myocarditis (n = 3); and hemorrhagic enteritis (n = 3). The placenta and uterus of the cow as well as the kidney, pancreas, and liver of her aborted fetus contained H. somni DNA and demonstrated histopathological evidence of histophilosis. All fetuses contained H. somni DNA in multiple organs. Coinfections of H. somni with B. abortus (n = 2), N. caninum (n = 2), BVDV (n = 1), and BoHV-1 (n = 1) were identified; two fetuses demonstrated three pathogens. These findings suggest that H. somni was associated with the spontaneous abortions and reproductive problems of these herds. However, the exact cause of fetal death might not be attributed only to H. somni in all aborted fetuses, since some of these were infected with other abortifacient agents.

Molecular survey of infectious agents associated with bovine respiratory disease in a beef cattle feedlot in southern Brazil

We investigated the occurrence of infectious pathogens during an outbreak of bovine respiratory disease (BRD) in a beef cattle feedlot in southern Brazil that has a high risk of developing BRD. Nasopharyngeal swabs were randomly collected from steers (n = 23) and assessed for the presence of infectious agents of BRD by PCR and/or RT-PCR assays. These included: Histophilus somni, Mannheimia haemolytica, Pasteurella multocida, Mycoplasma bovis, bovine respiratory syncytial virus (BRSV), bovine coronavirus (BCoV), bovine viral diarrhea virus (BVDV), bovine alphaherpesvirus 1 (BoHV-1), and bovine parainfluenza virus 3 (BPIV-3). Pulmonary sections of one steer that died with clinical BRD were submitted for pathology and molecular testing. The frequencies of the pathogens identified from the nasopharyngeal swabs were: H. somni 39% (9 of 23), BRSV 35% (8 of 23), BCoV 22% (5 of 23), and M. haemolytica 13% (3 of 23). PCR or RT-PCR assays did not identify P. multocida, M. bovis, BoHV-1, BVDV, or BPIV-3 from the nasopharyngeal swabs. Single and concomitant associations of infectious agents of BRD were identified. Fibrinous bronchopneumonia was diagnosed in one steer that died; samples were positive for H. somni and M. haemolytica by PCR. H. somni, BRSV, and BCoV are important disease pathogens of BRD in feedlot cattle in Brazil, but H. somni and BCoV are probably under-reported.

Bovine pulmonary lesions found in post-mortem inspection in slaughterhouse in state Paraná

Polyana Carolina Marino, Gercio Luiz Bonesi, Luiz Carlos Negri Filho, Daniella Furlan, Débora Nayara Augusto, Alexey Leon Gomel Bogado, Rogério Anderson Marcasso, Luiz Cesar da Silva, Marcelo Diniz dos Santos, Werner Okano 1 Docente. Departamento de Clínica Médica e Cirúrgica de Grandes Animais. UNINGA, Maringá, PR. pollyveterinaria@gmail.com 2 Docente. Medicina Veterinária. UNOPAR. Arapongas, PR. gercio.bonesi@agricultura.gov.br; rogeriomarcasso@gmail.com 3 Mestrando do Programa de Pós Graduação em Saúde de Produção de Ruminantes. PROSUP/CAPES. UNOPAR. Arapongas, PR. luiz.negri@hotmail.com; furlandanie@hotmail.com; debora_augusto@outlook.com 4 Docentes do Programa de Pós Graduação em Saúde de Produção de Ruminantes. UNOPAR. Arapongas, PR. alexey_leon@hotmail.com; silvaluizcesar@gmail.com; werner.okano@unopar.br 5 Docente do Programa de Pós Graduação em Biociência Animal. UNIC. Cuiabá, MT. marcelo.diniz@kroton.com.br __________________________________________________________________________________

Morfometria ovariana de bovinos de abate em Ibiporã – Paraná

O objetivo deste estudo foi avaliar a morfometria ovariana de 123 pares de ovarios (79 gestantes – G1 e 48 nao gestantes – G2) que foram coletadas em abatedouro em Ibipora, estado do Parana. As dimensoes ovarianas foram mensuradas usando um paquimetro digital medindo-se o comprimento e largura dos ovarios direito e esquerdo, a presenca de cistos foliculares e de corpo luteo (CL). Em vacas leiteiras foram observadas diferenca significativa para o tamanho do ovario direito em animais do grupo G1 ou G2 em relacao ao ovario esquerdo, cujas dimensoes eram menores em gado de corte. Foram observados 35,41% de cistos foliculares. Nao havendo diferenca estatistica na frequencia de corpo luteo nas aptidoes de corte e leite.

Liver abscess in cattle slaughter coming from 14 counties of north central mesorregion of Parana

The beef liver has important nutritional properties in food, but can transmit zoonoses important to humans. Service inspection of products of animal origin used to give food security in the consumption of these products. Liver abscesses occur by different etiologies in bovine liver. From the record of liver condemnations of cattle slaughter in abatoir under state inspection, 2,256 cattle slaughtered between August 2011 and March 2014, in Rolandia county from 14 counties of north central mesorregion Parana were detected 8,37% of liver abscesses. All 189 livers containing single or multiple abscesses were condemned by the standard inspection service, causing economic losses resulting decreased feed efficiency, and decreased carcass yield beyond the value of the viscera which is no longer marketed. Keys words: slaughterhouse, liver, Animal Standard Meat Inspection _______________________________ 1 . Acadêmico de Medicina Veterinária / UNOPAR Arapongas/PR. Bolsista PIBIC CNPq 2 . Acadêmico de Medicina Veterinária / UNOPAR Arapongas/PR. IC voluntário 3 . Acadêmico de Medicina Veterinária / UNOPAR Arapongas/PR. Bolsista PIBIT CNPq 4 . Mestrando Programa em Saúde e Produção de Ruminantes / UNOPAR UEL 5 . Docente de Medicina Veterinária / UNOPAR/PR – Arapongas 6 . Docente de Medicina Veterinária / UNOPAR Arapongas / PPG Saúde e Produção de Ruminantes UNOPAR – UEL 7 . Docente de Medicina Veterinária UNIC Cuiabá / PPG Biociência Animal Autor para correspondência * vetwerner@gmail.com Recebido em 30/006/2014. Aprovado em 20/09/2014

Morphometric characteristics of ovaries from cows collected at a abattoir in Ibipora - Paraná

The aim of the present study was to evaluate the ovarian morphometry from 123 pairs of ovaries cows (79 pregnant – G1 and 48 non-pregnant – G2) were collected in slaughterhouses in Ibipora, state Paraná. Ovarian dimensions were measured using digital pachymeter measured in thickness e width from the right and left ovaries, the follicle and the corpora lutea (CL) were examined. In dairy cows was observed significant difference for size of the right ovaries in G1 and G2 with the left ovary, the dimensions of ovaries right were lower in beef cows. It was observed 35,41% ovarian cysts. Its wasn’t difference significant the frequency of corpora lutea in the right and left side and dairy e beef cattle.

Hematological parameters of Holstein breed bovines: the influence of by the tuberculin comparative test

Hematological parameters of Holstein breed bovines: the influence of by the tuberculin comparative test Luiz Carlos Negri Filho1, Lucas Vinícius de Alcântara Andrade2, Pedro Henrique Newbery Chineze3, Mauricio Zampronio Affonso 4 , Marcelo Diniz dos Santos 5 , Flávio Antônio Barca Junior 6 , Alexey Leon Gomel Bogado 6 , Luiz Cesar da Silva 7 ,Werner Okano 7* _________________________________________________________________________________________________________________

Prevalence and classifications of claws injuries in dairy cattle in the region of Belo Horizonte-MG

1 Medico Veterinario autonomo em Santa Fe – PR 2 Aluno da Graduacao de Medicina Veterinaria UNOPAR, bolsista CNPq PIBIT 3 Mestrando PPG Academico Saude e Producao de Ruminantes UEL UNOPAR 4 Docentes PPG Mestrado Academico Saude e Producao de Ruminantes UEL UNOPAR e Medicina Veterinaria UNOPAR 5 Docente PPG Mestrado Academico Saude e Producao de Ruminantes UEL UNOPAR e Medicina Veterinaria UNOPAR. vetwerner@gmail.com ___________________________________________________________________________