Ronald A. Vitali

Guanosine-5′-monophosphate dioxanate: Preparation, characterization, and application

Abstract This paper reports, for the first time, the preparation and characterization of a unique crystalline complex of guanosine-5′-monophosphate, dioxane, and water (GMP-dioxanate) in a 1:1:1 molar ratio. The specificity of this complex is shown by the fact that no other solvent has been found which can replace dioxane. In addition, no other nucleotide or solvent combination has been found which forms a crystalline complex. GMP-Dioxanate is stable to heating and drying and is readily converted to the disodium salt. The compound has been fully characterized by physical, chemical, and biological analyses. Its crystallinity has been confirmed by both microscopic and X-ray analyses. Data on the optical rotation of solutions of GMP-dioxanate as a function of wavelength, concentration, and pH are described and compared with those of GMP and GMP·2Na·2H 2 O. This complex has provided a simplified procedure for the isolation of GMP from acid and salt mixtures, fermentation broths, and ribonucleic acid hydrolyzates.