Ronald Younes

Picrosirius Red Staining A Useful Tool to Appraise Collagen Networks in Normal and Pathological Tissues

Specific staining of the extracellular matrix components is especially helpful in studying tissue remodeling, particularly in the case of connective tissue pathologies. As developed by Junqueira and colleagues in 1979, specific staining by Picrosirius red is one of the most important stains to study collagen networks in different tissues. Under polarized light, collagen bundles appear green, red or yellow, and are easily differentiated from the black background, thus allowing for quantitative morphometric analysis. As Junqueira and colleagues point out, many studies use color staining to differentiate collagen bundles and to specify collagen types, yet other studies report that polarized colors only reflect fiber thickness and packing. Using a simple histological example, our study illustrates the inability of Picrosirius red staining to differentiate collagen types, since the absorbed amount of polarized light by this dye strictly depends on the orientation of the collagen bundles.

Biotribocorrosion (tribo-electrochemical) characterization of anodized titanium biomaterial containing calcium and phosphorus before and after osteoblastic cell culture

The purpose of this study was to investigate the relationship between the osteoblastic cells behavior and biotribocorrosion phenomena on bioactive titanium (Ti). Ti substrates submitted to bioactive anodic oxidation and etching treatments were cultured up to 28 days with MG63 osteoblast-like cells. Important parameters of in vitro bone-like tissue formation were assessed. Although no major differences were observed between the surfaces topography (both rough) and wettability (both hydrophobic), a significant increase in cell attachment and differentiation was detected on the anodized substrates as product of favorable surface morphology and chemical composition. Alkaline phosphatase production has increased (≈20 nmol/min/mg of protein) on the anodized materials, while phosphate concentration has reached the double of the etched material and calcium production increased (over 20 µg/mL). The mechanical and biological stability of the anodic surfaces were also put to test through biotribocorrosion sliding solicitations, putting in evidence the resistance of the anodic layer and the cells capacity of regeneration after implant degradation. The Ti osteointegration abilities were also confirmed by the development of strong cell-biomaterial bonds at the interface, on both substrates. By combining the biological and mechanical results, the anodized Ti can be considered a viable option for dentistry.

Lateral Sinus Grafting Approach: Overview and Recent Developments

With the increased popularity of dental implant therapy for the replacement of missing teeth, a need arose for a method to provide patients with bony support for these implants in cases where alveolar ridges volume were insufficient for implant placement.

Bone Lid Technique Using a Piezoelectric Device for the Treatment of a Mandibular Bony Lesion

Different techniques for the enucleation of jaw cyst lesion in the oral and maxillofacial regions have been proposed, including the bone lid technique. The purpose of this case report is to describe the case of a cystic lesion, approached with the bone lid technique performed using a piezoelectric device, with an 8-month clinical and radiographic follow-up. A 14-year-old male patient was treated for a suspicious lesion detected on a panoramic radiograph. The concerned area was surgically accessed, and a radiographically predetermined bony window was drawn, and the beveled bony lid was removed. The underlying lesion was enucleated and sent for pathology as a routine procedure, and the removed bony lid was repositioned in situ and secured with a collagen tape. Healing was uneventful with limited swelling and reduced pain. A complete radiographic bone healing at the previously diseased site was confirmed with an 8-month cone beam computed tomography (CBCT) scan with no buccal bone resorption nor ridge collapse. The bone lid technique with a piezoelectric device was noninvasive and atraumatic in this case. Further studies are needed and could lead to the adaptation of this approach as a possible standard of care.

Use of Grafting Materials in Sinus Floor Elevation: Biologic Basis and Current Updates

With the increased need for the replacement of missing teeth in the posterior maxilla through dental implant therapy, a need arose for a method to provide patients with a sufficient bony support in cases where maxillary sinuses residual height was deficient for implant placement. One of the aims of the SFE is the creation of vital bone to achieve the osseointegration of dental implants placed in the posterior maxilla. With this goal in mind, various bone substitutes (BS) have been used for SFE procedures, including autografts, allografts, xenografts, and alloplasts.

Clinical and Radiological Assessment and Planning in Sinus Floor Elevation

With relatively invasive surgical techniques such as sinus floor elevation (SFE) procedures, a precise clinical and radiographic diagnosis is required to perform a convenient dental implant planning.

Current State, Treatment Modalities, and Future Perspectives of Sinus Floor Elevation (SFE)

Nowadays, more than half (54.2 %) of the implant rehabilitations in the edentulous posterior maxilla are involved with an SFE procedure prior to implant placement (Seong et al. 2013).

Introduction and Scientific Background of Sinus Floor Elevation (SFE)

In a constantly aging society, the need for maxillary implant rehabilitation is increasing. In fact, the regeneration of the physiological function of the dento-maxillary system is crucial for improvement in life quality.

Crestal Sinus Floor Elevation (SFE) Approach: Overview and Recent Developments

Dental implant placement in the posterior maxilla remains a challenge due to maxillary sinus pneumatization. SFE procedures via lateral approach have overcome this obstacle, although it is considered to be a rather aggressive approach. In an attempt to reduce such morbidity, several techniques using osteotomes and different specialized instruments have been introduced to reach the sinus floor via crestal approach: Summers (Compendium 15:152, 1994a; Compendium 15:698, 1994b) described a one-stage crestal SFE approach originally indicated in the presence of a moderate residual bone height (RBH) and combined to simultaneous implant placement (OSFE, BAOSFE…), which was considered to be simpler and less invasive when compared to the lateral SFE. However, the main limitation of this “blind” crestal approach is the uncertainty of membrane integrity as a result of lack of visibility. Therefore, despite their high success rate, criticism on the safety of the crestal technique may be expressed.