Rosa María Valencia-Barrera

The role of meteorological factors in determining the annual variation ofAlternaria andCladosporium spores in the atmosphere of Palencia, 1990–1992

A study was made of the daily content ofAlternaria andCladosporium spores in the atmosphere in Palencia city (Spain) for three consecutive years 1990–1992.Alternaria andCladosporium represented 55% of the total identified spores, presenting an annual distributional pattern of which the maximum values were reached in summer. Multiple regression analyses showed a positive correlation between minimum temperature andCladosporium spore concentrations, while forAlternaria there was a positive correlation with maximum temperature and a negative correlation with precipitation. Duncans multiple range test among means ofAlternaria data indicated that winds coming from a northeasterly direction were associated with significantly higher concentrations the spores.

Airborne pollen and spores of León (Spain)

A qualitative and quantitative analysis of airborne pollen and spores was carried out over 2 years (from September 1987 to August 1989) in the city of León. Slides were prepared daily using a volumetric pollen trap, which was placed on the Faculty of Veterinary Science building (University of León) 12m above ground-level. Fifty-one pollen types were observed; the most important of these were: Cupressaceae during the winter,Pinus andQuercus in spring, and Poaceae, Leguminosae and Chenopodiaceae in the summer. The results also showed the existence of a rich mould spore assemblage in the atmosphere. The group of Amerospores (Penicillium, Aspergillus andCladosporium) as well as Dictyospores (Alternaria) were the most abundant;Puccinia was common in the air in August. Fluctuations in the total pollen and spores m3 of air were compared with meteorological parameters (temperature, relative humidity and rainfall). From the daily sampling of the atmosphere of León, considering the maximum and minimum temperature and duration of rainfall, the start of the pollen grain season was observed generally to coincide with a rise in temperature in the absence of rain.

Aerobiological study of fungal spores from Palencia (Spain)

A study of the concentration of fungal spores has been carried out in the atmosphere of Palencia town (NW Spain) during 1992. The volumetric method of filtration has been used. Half of the daily filter sample has been cultivated in Czapecdox-agar or Sabouraud-agar for the identification of fungal colonies, and the other half has been examined by optical microscopy. Several colonies belonging to 26 genera have been identified. Deuteromycetes (54%) and Zigomycetes (28%) are assembled in four genera, and Bacteria and Actynomycetes (18%) in three genera. The greatest concentrations occur forAspergillus (23%),Mucor (25%), followed byPenicillium (16%). The greatest diversity and abundance of fungal spores are found in September–December. The viable colonies are more abundant in Czapedox-agar culture, whereas Bacteria were more frequently found in Sabouraud medium.

Biogeography and bioclimatology in pollen forecasting

A comparative study of the atmospheric pollen content in two cities, located at similar latitudes, though in different continents, was undertaken. Special consideration was given to the meteorological determinants as shown by correlation analyses. In both localities, the contribution of Poaceae to the aerospora was quite distinct and varied from year to year. Analyses were carried out taking into account either years or cities separately or combined, and at two time scales: daily or weekly counts. Maximum temperature was the major meteorological determinant for daily grass pollen concentration values. Owing to its more arid climate, grass populations in Leo´n seem to react more to sunshine hours. Relative humidity and precipitation had a negative influence but at different time scales in the two localities. In Leo´n, they are related to everyday events such as convective storms, while in Montre´al, the same events are related to low-pressure systems that have the same time scale as air masses. We found that wind speed was the only factor that acted differently in Montre´al and Leo´n. In Leo´n, wind speed has the well-known dilution impact, while in Montre´al, it has a positive impact by increasing pollen shedding from the anthers. Wind speed has therefore a stronger influence at two different stages of the aerobiological pathway in both localities.

Characterization of honeys by melissopalynology and statistical analysis

We analyzed pollen from 89 honey samples, collected in Leon and Palencia provinces (NW Spain). According to their pollen spectra, 46 were considered monofloral. The most abundant monofloral honeys were Erica types followed by Castanea, Centaurea, Reseda and Helianthus. One hundred and forty-two different pollen types were recorded, belonging to 47 families. Fifty-five of them reached percentages over 3% in at least one sample, while the other 87 types never exceeded 3% in any of the 89 samples. The families that were present in the highest number of samples were Fabaceae, Rosaceae, Cistaceae and Asteraceae. Plant families that had the highest percentages were Fabaceae, Ericaceae, Asteraceae, and Rosaceae. The pollen types that appeared in most samples were Rubus ulmifolius (73 samples), Cytisus scoparius (70) and Mentha aquatica (62); the pollen types that had the highest relative abundance were Erica arborea, Lotus corniculatus, Cytisus scoparius. The pollen types of the Ericaceae family, Jasione montana...

Molecular aerobiology - Plantago allergen Pla l 1 in the atmosphere

INTRODUCTION Exposure to airborne pollen from certain plants can cause allergic disease, but allergens can also be found in non-pollen-bearing fractions of ambient air. This may explain why the allergic response in susceptible patients does not always coincide with the presence and magnitude of airborne pollen counts. Plantago pollen is an important cause of pollinosis in northern Mediterranean countries, but it is difficult to determine its incidence in allergies because Plantago pollen appears in the atmosphere at the same time as grass pollen. OBJECTIVE The study aimed to investigate the relationship between the atmospheric concentration of Pla l 1 aeroallergen and Plantago pollen, and its incidence in a population group. MATERIALS AND METHOD Pollen was sampled using a Hirst-type volumetric trap (Burkard) and Burkard Cyclone sampler (Burkard) for Pla l 1 allergen. Allergen was determined with a Pla l 1-specific ELISA. Serum-specific IgE levels to several plant allergens were measured with the EAST system. RESULTS The aerobiological dynamics of Plantago pollen grains and Pla l 1 did not follow the same trend, whereas the sum of Plantago with some other pollen types showed a more similar behaviour. Of the 118 subjects tested, sera from 52 contained IgE to Plantago pollen, but only 5 were monosensitized. CONCLUSIONS The presence of Pla l 1 in the atmosphere depends not only on Plantago pollen but also on the pollen of other species from the Oleaceae family. Knowledge of the behaviour of allergen Pla l 1 in the atmosphere can help understand better asthma exacerbations associated with aeroallergens.

Detection and release of allergenic proteins in Parietaria judaica pollen grains

Summary.Rapid diffusion of allergenic proteins in isotonic media has been demonstrated for different pollen grains. Upon contact with stigmatic secretion or with the mucosa of sensitive individuals, pollen grains absorb water and release soluble low-molecular-weight proteins, these proteins enter in the secretory pathway in order to arrive at the cell surface. In this study we located allergenic proteins in mature and hydrated-activated pollen grains of Parietaria judaica L. (Urticaceae) and studied the diffusion of these proteins during the first 20 min of the hydration and activation processes. A combination of transmission electron microscopy and immunocytochemical methods was used to locate these proteins in mature pollen and in pollen grains after different periods of hydration and activation processes. Activated proteins reacting with antibodies in human serum from allergic patients were found in the cytoplasm, wall, and exudates from the pollen grains. The allergenic component of these pollen grains changes according to the pollen state; the presence of these proteins in the exine, the cytoplasm, and especially in the intine and in the material exuded from the pollen grains, is significant in the hydrated-activated studied times, whereas this presence is not significant in mature pollen grains. The rapid activation and release of allergenic proteins of P. judaica pollen appears to be the main cause of the allergenic activity of these pollen grains.

Pollen and organoleptic analysis of honeys in Leon province (Spain)

According to the pollen spectra recorded, most of them were monofloral (23) and 16 were multifloral. Erica honeys were the most abundant monofloral samples (9), Castanea sativa came next with 5; there were 3 samples of Helianthus annuus and 3 others of Lotus corniculatus and 1 of Rubus ulmifolius, Echium and Scrophularia canina pollen type respectively. The monofloral chestnuts honeys presented a minor pollen diversity. 108 pollen types were recorded, which belonged to 41 families. Fabaceae, Rosaceae and Ericaceae were the most represented families in the honeys studied. Cytisus scoparius and Rubus ulmifolius were the pollen types, which appeared in the highest number of samples. Certain pollen types such as Lavandula stoechas and Cistus ladanifer can be used as indicator or key elements in order to recognise the origin zone of honeys in the biogeographical Mediterranean region. As regards organoleptic analyses, most of our honeys (multiflorales, Erica, Rubus ulmifolius, Castanea sativa), samples are brown, not very sweet and present a homogeneous aspect, the other honeys have lighter colours, are sweeter and also have an homogeneous aspect (Helianthus annuus, Lotus corniculatus, Echium and Scrophularia canina -type).

Palinologia do componente herbáceo na atmosfera de Caxias do Sul, RS, Brasil

Este estudo foi realizado na cidade de Caxias do Sul de 1/janeiro/2001 a 31/dezembro/2002 e objetivou identificar os tipos polinicos de taxons herbaceos presentes na atmosfera e suas oscilacoes sazonais. A cidade esta localizada na Encosta Superior Nordeste, Estado do Rio Grande do Sul. Seu clima e tropical pluvisazonal e apresenta tres diferentes tipos de vegetacao: Floresta Ombrofila Mista, Floresta Decidual e Campo. O monitoramento polinico foi realizado utilizando o captador volumetrico de succao Burkard®, instalado no topo do Hospital Geral a uma altura aproximada de 20 m. Durante o periodo de estudo foram identificados 40 tipos polinicos, sendo 11 de taxons herbaceos, representando 23,22% do total de graos coletados. A concentracao maxima das taxons herbaceos registrou-se nos meses de outubro e novembro. O tipo polinico Poaceae R.Brown apresentou a maior incidencia, seguido por Cyperaceae Juss., Plantago L., Amaranthus L., Rumex L., Iridaceae Juss., Brassicaceae Burnett, Apiaceae Lindl., Alternanthera Forssk., Convolvulaceae Juss. e Caryophyllaceae Juss.

Spanish honeys with quality brand: a multivariate approach to physicochemical parameters, microbiological quality, and floral origin

This study consisted of a palynological, microbiological, and physicochemical characterization of fifteen samples of Spanish honey sold under quality brands with different botanical and geographical origins from two consecutive harvest years (2010 and 2011). Eight of the fifteen honey samples were classified as monofloral honey from botanical origins Persea americana, Castanea sativa, Rosmarinus officinalis, Eucalyptus sp., and Thymus sp. With regard to microbiological analyses, mold, and yeast counting, Staphylococcus aureus, Salmonella, sulfite-reducing clostridia, and Escherichia coli were not detected in any of the samples. Aerobic mesophilic microorganisms were detected only in some samples and the counts in these cases were low. Despite the great variability between samples, the results obtained in the physicochemical analysis were consistent with the limits set by the Council Directive 2001/100. Honey samples showed high variability between two consecutive harvests, since, even if they had similar geographical origins they showed different nectar floral origins. Mieles españolas con marca de calidad: un enfoque multivariado de parámetros fisicoquímicos, calidad microbiológica y origen floral Este estudio consistió en la caracterización botánica, microbiológica y fisicoquímica de quince muestras de miel españolas acogidas a marcas de calidad diferenciada de diferentes orígenes botánicos y geográficos y procedentes de dos cosechas consecutivas (años 2010 y 2011). Ocho de las quince muestras de miel estudiadas fueron clasificadas como mieles monoflorales de Persea americana, Castanea sativa, Rosmarinus officinalis, Eucalyptus sp. y Thymus sp. En relación a los análisis microbiológicos los recuentos de mohos y levaduras, Staphylococcus aureus, Salmonella, clostridios sulfito reductores y Escherichia coli no fueron detectados en ninguna de las muestras. Se detectaron microorganismos aerobios mesófilos solo en algunas muestras y en este caso los recuentos fueron bajos. Aunque se detectaron coliformes, estos podrían estar asociados a un origen ambiental. A pesar de la gran variabilidad entre las muestras, los resultados obtenidos en el análisis fisicoquímico se encontraron dentro de los límites establecidos por la Directiva del Consejo 2001/100. Las muestras de miel mostraron una gran variabilidad entre cosechas porque aunque tuvieron un mismo origen geográfico, hubo diferencias en su origen floral.