Rosemary B. Bassey

Curcuma Longa: Staining Effect on Histomorphology of the Testis

Curcuma Longa: Staining Effect on Histomorphology of the Testis Background: The use of non-allergic, non-toxic and eco-friendly natural dyes has become a matter of significant importance due to the increased environmental awareness in order to avoid some hazardous synthetic dyes. Method: The ethanolic extract of Curcuma longa was diluted using 1% acetic acid in 70% ethanol to a concentration of 0.2 g/ml. It was used to stain histological sections of the testes for 15 minutes. Curcuma longa was also used as a counter stain for Haematoxylin. Phytochemical constituents were investigated. Results: The Curcuma longa dye distinctly stained the seminiferous epithelium and interstitium yellow. Curcuma longa provided a good counter stain for Haematoxylin, taking up the acidic staining characteristics with Haematoxylin staining the basic staining characteristics. Phytochemical screening revealed the presence of saponins, alkaloids, tannins and flavonoids. Conclusion:Curcuma longa has good potential for use as a counter stain for Haematoxylin in the staining of tissues in lieu of Eosin.

Conditional Prerequisites for Microchannel Cytologic Analysis on Wet Mount (Fluid-Based) Biopsies

Advanced capabilities in genomic sequencing developed in the research sector will soon enter the clinical arena. Issues such as the proportioning of patient specimen material for traditional bright‐field microscopic evaluation or dedication for molecular analysis will intensify, particularly in situations of small core biopsies. Microfluidics appears aptly suited as a platform capable of allowing traditional cytologic diagnostics and downstream molecular analysis from the same specimen. However, clarification is needed to determine that forces which act on cells in a fluidic environment do not drastically alter their cytologic features.

An evaluation of synthetic and natural supravital stains for the cytological examination of dissociated cells in a microfluidic channel.

Advances in biotechnology will result in paradigm shifts in both oncology and diagnostics. In pathology, methods such as microfluidics are being explored as delivery tools so that processed cells can serve dual purposes: conventional cytology‐based diagnostics and recovery of the same cells for molecular assays. This wet mount‐based approach to diagnosis will require staining of these cells by supravital dyes. This study was undertaken to determine the optimal supravital stain for the examination of cells in the wet mount preparations present in microfluidic platforms.

Staining effect of Hibiscus sabdariffa extract on sperm cell morphology of Sprague–Dawley rats

Abstract There is increasing awareness among people towards natural products. Due to their non-toxic properties, low pollution, and lower side effects, natural dyes are used in many day-to-day products. Although the African continent possesses plentiful plant resources, only a small amount has been exploited so far. This study evaluated the use of Hibiscus sabdariffa as a stain to evaluate sperm morphology. Following liquefaction, 10 μl of semen was spread onto glass slides and allowed to air-dry at room temperature. The smear was fixed for 15 minutes in methanol. The sperm morphology was analyzed by staining 10 slides of the smears with eosin (control) and the ethanolic extract of Hibiscus sabdariffa dye was used to stain the sperm cells. The smears were air-dried and viewed at magnification of ×400. Phytochemical and chromatographic analyses were carried out. The sperm cells were stained in shades of reddish brown. Preliminary phytochemical screening of Hibiscus sabdariffa revealed that it contains alkaloids, saponins, flavonoids, and tannins. Hibiscus sabdariffa has potential for use as a stain for study of sperm morphology.

Effect of Preconception Gamma Irradiation on Morphometric Assessment of Adult Female Mice and Embryo

El objetivo fue estudiar el efecto de la irradiacion gamma antes de la concepcion sobre la morfometria macroscopica de ratones hembra adultos y los embriones de sus crias. Veinte y siete ratones, 18 hembras y 9 machos, divididos en 3 grupos (control, sin irradiacion e irradiado) con 6 hembras y 3 machos cada uno en proporcion 2:1. Una dosis de radiacion gamma de 1 Gy/min fue aplicada a uno de los ratones expuestos por un equipo Cobalt 60, Theratron modelo 780c, Atomic Energy of Canada Limited (AECL) en el departamento de radioterapia del Hospital University College de Ibadan. Todos los animales se aparearon 1 semana despues de la irradiacion. Se confirmaron los tapones vaginales, y las hembras prenadas fueron sacrificadas en el dia 14 de la gestacion por inhalacion de cloroformo. La morfologia general de los ratones hembras y sus camadas fueron evaluadas y analizadas estadisticamente. Un total de 113 embriones se recolectaron en todos los grupos, 54 del grupo control, 50 del grupo no irradiados y 9 del grupo irradiado. Las evaluaciones morfologicas macroscopicas de los fetos fueron estadisticamente significativas (p<0,05) para los 3 grupos de comparacion. Estos hallazgos sugieren que una irradiacion previa a la concepcion afecta a la morfologia de los ratones hembra y su progenie.

Factors influencing extract of Hibiscus sabdariffa staining of rat testes

Abstract Some plant extracts can be used in biology and medicine to reveal or identify cellular components and tissues. We investigated the effects of time and concentration on staining of histological sections of rat testes by an acidified extract of Hibiscus sabdariffa. An ethanolic extract of H. sabdariffa was diluted using 1% acetic acid in 70% ethanol to stain histological sections of testes at concentrations of 0.2, 0.1 and 0.05 g/ml for 5, 10, 15, 30, 45 and 60 min. The sections of testes were stained deep red. The staining efficiency of H. sabdariffa was greater at a high concentration and required less time to achieve optimal staining. H. sabdariffa is a strongly basic dye that can be used for various diagnostic purposes. Staining time and concentration must be considered to achieve optimal results.