Roxanne E. Buxton

Reliability and Validity of Panoramic Ultrasound for Muscle Quantification

This study examined the reliability and validity of using customized templates to acquire panoramic ultrasound (US) images for determining cross-sectional area (CSA) and volume in the vastus lateralis (VL), rectus femoris (RF), medial gastrocnemius (MG) and lateral gastrocnemius (LG). Panoramic US and magnetic resonance imaging (MRI) images were analyzed by two trained investigators. The inter-experimenter reliability (coefficient of variation [CV]) of panoramic US ranged from 2.4% to 4.1% and the intraclass correlation (ICC) ranged from 0.963 to 0.991, whereas the inter-experimenter CV of MRI ranged from 2.8% to 3.8% and the ICC from 0.946 to 0.986. Bland-Altman plots demonstrated high agreement between US and MRI; however, values obtained from MRI were systematically larger than those obtained from US. The present results indicate that using a customized US template provides reliable measures of leg muscle CSA and, thus, could be used to characterize muscle CSA and volume.

Test battery designed to quickly and safely assess diverse indices of neuromuscular function after unweighting.

Spiering, BA, Lee, SMC, Mulavara, AP, Bentley, JR, Buxton, RE, Lawrence, EL, Sinka, J, Guilliams, ME, Ploutz-Snyder, LL, and Bloomberg, JJ. Test battery designed to quickly and safely assess diverse indices of neuromuscular function after unweighting. J Strength Cond Res 25(2): 545-555, 2011-Adequately describing the functional consequences of unweighting (e.g., bed rest, immobilization, spaceflight) requires assessing diverse indices of neuromuscular function (i.e., strength, power, endurance, central activation, force steadiness). Additionally, because unweighting increases the susceptibility of muscle to damage, testing should consider supplementary safety features. The purpose of this study was to develop a test battery for quickly assessing diverse indices of neuromuscular function. Commercially available exercise equipment was modified to include data acquisition hardware (e.g., force plates, position transducers) and auxiliary safety hardware (e.g., magnetic brakes). Ten healthy, ambulatory subjects (31 ± 5 years, 173 ± 11 cm, 73 ± 14 kg) completed a battery of lower- and upper-body neuromuscular function tests on 3 occasions separated by at least 48 hours. The battery consisted of the following tests, in order: (1) knee extension central activation, (2) knee extension force steadiness, (3) leg press maximal strength, (4) leg press maximal power, (5) leg press power endurance, (6) bench press maximal strength, (7) bench press force steadiness, (8) bench press maximal power, and (9) bench press power endurance. Central activation, strength, rate of force development, maximal power, and power endurance (total work) demonstrated good-to-excellent measurement reliability (SEM = 3-14%; intraclass correlation coefficient [ICC] = 0.87-0.99). The SEM of the force steadiness variables was 20-35% (ICC = 0.20-0.60). After familiarization, the test battery required 49 ± 6 minutes to complete. In conclusion, we successfully developed a test battery that could be used to quickly and reliably assess diverse indices of neuromuscular function. Because the test battery involves minimal eccentric muscle actions and impact forces, the potential for muscle injury has likely been reduced.

Panoramic ultrasound: a novel and valid tool for monitoring change in muscle mass

The strong link between reduced muscle mass and morbidity and mortality highlights the urgent need for simple techniques that can monitor change in skeletal muscle cross‐sectional area (CSA). Our objective was to examine the validity of panoramic ultrasound to detect change in quadriceps and gastrocnemius size in comparison with magnetic resonance imaging (MRI) in subjects randomized to 70 days of bed rest (BR) with or without exercise.

Individual Variability in Aerobic Fitness Adaptations to 70 Days of Bed Rest and Exercise Training: 2448 June 3, 10

Change in maximal aerobic capacity (VO2pk) in response to exercise training and disuse is highly variable among individuals. Factors that could contribute to the observed variability (lean mass, daily activity, diet, sleep, stress) are not routinely controlled in studies. The NASA bed rest (BR) studies use a highly controlled hospital based model as an analog of spaceflight. In this study, diet, hydration, physical activity and light/dark cycles were precisely controlled and provided the opportunity to investigate individual variability. PURPOSE. Evaluate the contribution of exercise intensity and lean mass on change in VO2pk during 70-d of BR or BR + exercise. METHODS. Subjects completed 70-d of BR alone (CON, N=9) or BR + exercise (EX, N=17). The exercise prescription included 6 d/wk of aerobic exercise at 70 - 100% of max and 3 d/wk of lower body resistance exercise. Subjects were monitored 24 hr/d. VO2pk and lean mass (iDXA) were measured pre and post BR. ANOVA was used to evaluate changes in VO2pk pre to post BR. Subjects were retrospectively divided into high and low responders based on change in VO2pk (CON > 20% loss, n=5; EX >10% loss, n=4, or 5% gain, n=4) to further understand individual variability. RESULTS. VO2pk decreased from pre to post BR in CON (P<0.05) and was maintained in EX; however, significant individual variability was observed (CON: -22%, range: -39% to -.5%; EX: -1.8%, range: -16% to 12.6%). The overlap in ranges between groups included 3 CON who experienced smaller reduction in VO2pk (<16%) than the worst responding EX subjects. Individual variability was maintained when VO2pk was normalized to lean mass (range, CON: -33.7% to -5.7%; EX: -15.8% to 11%), and the overlap included 5 CON with smaller reductions in VO2pk than the worst responding EX subjects. High responders to disuse also lost the most lean mass; however, this relationship was not maintained in EX (i.e. the largest gains/losses in lean mass were observed in both high and low responders). Change in VO2pk was not related to exercise intensity. CONCLUSION. Change in VO2pk in response to disuse and exercise was highly variable among individuals, even in this tightly controlled study. Loss in lean mass accounts for a significant degree of variability in the CON; however, training induced gains in VO2pk appear unrelated to lean mass or exercise intensity.

Effects Of Reduced Strength On Self-selected Pacing For Long-duration Activities: 2668 May 29, 2

Strength and aerobic capacity are predictors of astronaut performance for extravehicular activities (EVA) during exploration missions. It is expected that astronauts will selfselect a pace below their ventilatory threshold (VT). PURPOSE: To determine the percentage of VT that subjects selfselect for prolonged occupational tasks. METHODS: Maximal aerobic capacity and a variety of lowerbody strength and power variables were assessed in 17 subjects who climbed 480 rungs on a ladder ergometer and then completed 10 km on a treadmill as quickly as possible using a selfselected pace. The tasks were performed on 4 days, with a weighted suit providing 0% (suit fabric only), 40%, 60%, and 80% of additional bodyweight (BW), thereby altering the strength to BW ratio. Oxygen consumption and heart rate were continuously measured. Repeated measures ANOVA and posthoc comparisons were performed on the percent of VT values under each suited condition. RESULTS: Subjects consistently selfpaced at or below VT for both tasks and the pace was related to suit weight. At the midpoint for the ladder climb the 80% BW condition elicited the lowest metabolic cost (19+/-14% below VT), significantly different than the 0% BW (3+/-16%, P=0.002) and the 40% BW conditions (5+/-22%, P=0.023). The 60% BW condition (13+/-19%) was different than the 40% BW condition (P=0.034). Upon completion of the ladder task there were no differences among the conditions (0%BW: 3+/-18%; 40%BW: 3+/-21%; 60%BW: 8+/-25%; 80%BW: 10+/-18%). All subjects failed to complete 5km at 80%BW. At the midpoint of the treadmill test the three remaining conditions were all significantly different (0%BW: 20+/-15%; 40%BW: 33+/-15%; 60%BW: 41+/-19%). Upon completion of the treadmill test the 60% BW condition (38+/-12%) was significantly different than the 40% BW (28+/-15%, P=0.024). CONCLUSIONS: Decreasing relative strength results in progressive and disproportionate decreases (relative to VT) in selfselected pacing during longduration activities. Thus, during prolonged, endurancetype activities, large reductions in strength cause notable performance decrements despite no changes in aerobic capacity. These data highlight the importance of both aerobic capacity and muscle strength to the performance of prolonged EVA in exploration mission scenarios.

Analysis of Skeletal Muscle Metrics as Predictors of Functional Task Performance: 2895

Performance Jeffrey W. Ryder, Roxanne E. Buxton, Elizabeth Redd, Melissa Scott-Pandorf, Kyle J. Hackney, James Fiedler, Robert J. Ploutz-Snyder, Jacob J. Bloomberg Lori L. Ploutz-Snyder, FACSM. Universities Space Research Association, Houston, TX, University of Houston, Houston, TX, Wyle Integrated Science and Engineering Group, Houston, TX, Syracuse University, Syracuse, NY, NASA Johnson Space Center, Houston, TX PURPOSE: The ability to predict task performance using physiological performance metrics is vital to ensure that astronauts can execute their jobs safely and effectively. This investigation used a weighted suit to evaluate task performance at various ratios of strength, power, and endurance to body weight. METHODS: Twenty subjects completed muscle performance tests and functional tasks representative of those that would be required of astronauts during planetary exploration (see table for specific tests/tasks). Subjects performed functional tasks while wearing a weighted suit with additional loads ranging from 0-120% of initial body weight. Performance metrics were time to completion for all tasks except hatch opening, which consisted of total work. Task performance metrics were plotted against muscle metrics normalized to “body weight” (subject weight + external load; BW) for each trial. Fractional polynomial regression was used to model the relationship between muscle and task performance. RESULTS: The R values for normalized muscle performance versus task performance are presented in the table below. Supine Seat Egress and Walk Object Pick Up and Carry Ladder Treadmill Hatch Opening

Reliability of a Test Battery Designed for Quickly and Safely Assessing Diverse Indices of Neuromuscular Function: 844

Dependent VariablerSEMrICCMaximal Isometric Force Maximal Isometric Force (N) 3% 0.99rRate of Force Development 14% 0.93(N/ms)Force Steadiness Force Steadiness with Visual 20% 0.60Feedback (CV)rForce Steadiness without Visual 33% 0.26Feedback (CV)Power Endurance Maximal Power (W) 9% 0.97rFatigue Index (%) 16% 0.62rrTotal Work (J)r4%r0.99r