Rudy Agung Nugroho

Dietary organic selenium improves growth, survival and resistance to vibrio mimicus in cultured marron, cherax cainii (Austin, 2002)

To determine the effects of dietary organic selenium (OS) supplementation on the growth performance and immune competence of marron, Cherax cainii (Austin, 2002), a group of marron were fed 0.2 g kg(-1) of Sel-Plex(®) supplemented basal diet and then compared with another group (control) of marron fed basal diet without any supplementation. After 90 days of feeding, final weight, average weekly gains (AWG), relative gain rate (RGR), specific growth rate (SGR), survival, total and differential haemocyte counts (THC and DHC), were compared between the two groups. Surviving marron from each group were then divided into three sub-groups (three tanks per sub-group with seven marron per tank); (1) first sub-group was injected with 20 μL of 3.24 × 10(6) cfu Vibrio mimicus; (2) the second sub-group was injected with 20 μL normal saline and (3) the third sub-group was not subjected to injection and became the control group. THC, DHC, neutral red retention time (NRRT) and Vibrio ranks of post-injected marron were evaluated for 96 h, at every 24-h interval. The results showed that after 90 days of feeding, final weight, AWG, RGR, SGR, survival, THC, proportion of hyaline cells of OS-fed marron were significantly higher (P < 0.05) than the control group, whereas proportion of granular and semigranular cells were not affected by dietary OS. After challenging with V. mimicus, survival rate of marron without dietary OS significantly decreased (P < 0.05) as compared to the control group of marron. THC of marron in all sub-groups were significantly reduced (P < 0.05) after the challenge. However, THC and granular cells of sub-groups fed OS were higher than other sub-groups. Vibrio ranks and NRRT of marron fed OS were significantly lower and slower, respectively, than marron fed without OS. These findings demonstrated the benefits of OS inclusion in the marron diet in terms of growth, health and disease resistance.

Terminalia catappa L. extract improves survival, hematological profile and resistance to Aeromonas hydrophila in Betta sp.

Abstract To determine the effects of Terminalia catappa extract (TCE) immersion on the survival and hematological profile of Betta sp, a group of fish was immersed in 500 ppm TCE and compared with the control group. After three days of immersion, survival, leukocyte (WBC), erythrocyte (RBC), hemoglobin (Hb), hematocrit (Hct), platelet (PLT), and differential leukocyte counts were compared between the two groups. Surviving fish from each group were then divided into three subgroups: subgroup without injection; subgroup injected with normal saline; subgroup injected with Aeromonas hydrophila. Survival, WBC, RBC, Hb, Hct, PLT, the percentage and number of lymphocyte, monocyte, and granulocytes post injection were evaluated for 48 h. The results showed that the survival of immersed fish was significantly higher than that in the control. No significant differences in the hematological profile were noted between the control and the immersed fish. The WBC of control subgroup (A. hydrophila injection) was significantly increased after 24 h. The fish immersed and injected with A. hydrophila had the highest PLT. The number of lymphocytes of all subgroups of fish was stable while the percentage of monocytes and granulocytes of the subgroups of immersed fish were increased. This finding suggested that 500 ppm of TCE is beneficial for improving survival, blood profile, and resistance to A. hydrophila.

Effects of propolis (Trigona sp.) extract supplementation on the growth and blood profile of Pangasius djambal

The study was conducted to examine the effects of propolis extract (PE) on the growth (G), growth rate (GR), specific growth rate (SGR) and blood profile (erythrocyte, leukocyte, and hemoglobin) of catfish (Pangasius djambal). five groups of fish with three replicates, containing 10 fish each group were fed various concentration of PE, viz 2 (P1), 4 (P2), 6 (P3), 8 (P4) and 10 (P5) g kg−1 of PE in a basal diet and compared to control (C) fish without PE at a rate 3% of body weight for 4 weeks. At the end of the trial, G, GR, SGR, and blood profile (erythrocyte, leukocyte, and hemoglobin) of all groups of fish were examined. The results showed that PE in the fish diet significantly increased G, GR, SGR, erythrocyte, hemoglobin while leukocyte was not affected by dietary any concentration of PE. The dietary 10 g kg−1 PE in the diet of fish showed the highest growth while the highest number of erythrocyte and hemoglobin activity was found in the fish fed 6 g kg−1 PE in the diet. These findings suggested that...

Antimicrobial activity of Terminalia catappa brown leaf extracts against Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853

The aim of this study was to determine the effects of various concentration of Terminalia catappa brown leaves extract which can inhibit the growth of Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853. The crushed-brown leaves of Terminalia catappa was extracted using 95% ethanol, filtered, and evaporated. The dried T. catappa extract was used to identify phytochemical content qualitatively. Total phenolic and flavonoid contents were also measured quantitatively from dried extract. The dried extracts were also dissolved in sterile aquadest and serial dilutions were prepared to final concentration of 30, 60 and 90%. A disc diffusion method was used to evaluate the antibacterial activity of various concentrations of ethanol extract of brown leaves of T. catappa. Inhibition zone diameter was measured to determine antibacterial activity. Gentamycin sulfate and distilled water were used as positive and negative controls, respectively. Dried ethanolic extract of brown T. catappa leaves contained flavonoid, quinon, phenolic, triterpenoid, and tannin. A total of 208.722 mg gallic acid equivalent/g extract of total phenolic and 35.7671 mg quercetin equivalent/g extract of total flavonoid were also found in the dried extract. The inhibition zone diameters of ethanolic extracts ranged from 1.73 to 9.06 mm ( S. aureus) and from 1.83 to 6.5 mm ( P. aeruginosa). The higher concentration of extract, the wider the inhibition zone diameters for both bacteria. P. aeruginosa was more resistant to high concentrations of extract (90%) than S. aureus. Ethanolic extracts of the brown leaves of T. catappa had different antibacterial effects against S. aureus and P. aeruginosa. The higher the concentration of extract, the wider the inhibition zone diameter for both bacteria. P. aeruginosa was more resistant to high concentrations of ethanolic extracts of the brown leaves of T. catappa.

Effect of various organic acid supplementation diets on Clarias gariepinus BURCHELL, 1822: Evaluation of growth, survival and feed utilization

Background: The purpose of the current study was to determine the growth status, survival and feed utilization of catfish ( Clarias gariepinus BURCHELL, 1822) fed various organic acid supplementations. Methods: In total, 1600 fish were randomly distributed into 20 tanks and fed different types of diet: A, control diet without organic acid supplementation; B, control diet supplemented with 0.05% formic, acetic, and propionic acid; C, control diet supplemented with 0.1% formic, acetic, and propionic acid; D, control diet supplemented with 0.05% butyric acid; E, control diet supplemented with 0.01% butyric acid. The control diet was a commercial diet, containing 35% crude protein, 8.58% crude fat, and 2.75% fibre. All fish were fed using a satiation method, three times per day for 56 days. At the end of the trial, growth, survival and feed utilization were determined. Water quality parameters during the trial were also measured once a week. Results: Fish fed diet type D had the significantly lowest ( P<0.05) final weight (FW), weight gain (WG), and specific growth rate (SGR) of all diets. Similar FW, WG, and SGR were found for fish fed diets A-C and E. Meanwhile, the feed conversion ratio, feed efficiency, and survival rate of fish were not affected by any types of diet. The water quality parameters were not significantly different between tanks and weeks: dissolved oxygen 6.79-6.81 mg L -1, pH 7.11-7.19, water temperature 28.97-29.32°C, nitrite (NO 2) content 0.48- 0.50 mg L -1, and ammonia (NH 3) content 0.064-0.066 mg L -1. Conclusion: The supplementation of 0.05% butyric acid in the diet of C. gariepinus for 56 days reduced the growth performance of the fish. However, supplementation of an organic acid in the diet of C. gariepinus had no impact on feed utilization, survival, and water quality parameters.