Ruixin Guo

Phytoplankton toxicity of the antibiotic chlortetracycline and its UV light degradation products.

Two common freshwater phytoplankton species Microcystis aeruginosa and Scenedesmus obliquus were employed as test organisms to investigate the toxic effects of chlortetracycline widely used in human medicine and veterinary as antibiotic. Toxicity assays were performed into two parts: antibiotic toxicity test and antibiotic degraded products toxicity test. In general, chlortetracycline had significantly toxic effect on population growth and chlorophyll-a accumulation of two phytoplankton. Although M. aeruginosa had ability to grow after exposed to chlortetracycline at 0.5 mg L(-1), its photosynthesis function was also disrupted. Compared with the data in two phytoplankton species, the chlorophyceae was more sensitive than the cyanophyceae. The adverse effect on S. obliquus was stronger than that on M. aeruginosa with increasing concentrations. In addition, for M. aeruginosa, regardless of the UV light degradation time, the treated chlortetracycline also had adverse effect on population growth and chlorophyll-a accumulated. The degraded chlortetracycline under any treatment time was more toxic for S. obliquus than chlortetracycline itself excluding under 24 h. However, the correlation between the toxicity and degradation time was not clear and toxicity enhanced in fact did not follow the increase or decrease in degradation time. Our study showed that the antibiotic chlortetracycline and its degraded products had adverse effect on freshwater phytoplankton, the former has not been reported before and the latter has been overlooked in other research in the past.

The swimming speed alteration of two freshwater rotifers Brachionus calyciflorus and Asplanchna brightwelli under dimethoate stress

Two common freshwater rotifer species Brachionus calyciflorus and Asplanchna brightwelli were employed as test organisms to investigate the toxic effects of the widely used organophosphate pesticide, dimethoate. The swimming angular speed and linear speed alteration of two rotifers were evaluated under the toxic stress in four concentrations (0.4, 0.8, 1.2, and 1.6 mg L(-1)). For B. calyciflorus, the rotifer swimming angular speed and linear speed were both adversely affected as a function of the toxicant concentrations. After a 2h exposure, the angular speeds at four concentrations were 39.37, 30.74, 26.68 and 23.96° s(-1), 65.30%, 50.98%, 44.25% and 39.74% of that of the control, respectively, while the mean linear speed decreased from 194.80 to 91.85×10(-3) mm s(-1), which was 70.12%, 48.14%, 34.02% and 33.06% of that of the control (277.82×10(-3) mm s(-1)), respectively. The pesticide also significantly inhibited the swimming angular speed of A. brightwelli. After a 2h exposure, the angular speeds of this rotifer at four concentrations were 39.37, 30.74, 26.68 and 23.96° s(-1), only 22.99%, 17.16%, 16.21% and 13.63% of that of the control (170.80° s(-1)), respectively. Compared with the results of B. calyciflorus, A. brightwelli was more sensitive on the swimming angular speed when exposed to the toxicant. It implied that A. brightwelli should be an alternative candidate model species about the toxicities of aquatic pollutants. In addition, when the rotifer A. brightwelli was exposed to four pesticide concentrations, the swimming linear speed displayed symptoms of hormesis, characterized by the conversion of low-concentration stimulate to high-concentration inhibition. Our results show that dimethoate had a significant effect on swimming of freshwater rotifers.

The process-dependent impacts of dimethoate on the feeding behavior of rotifer.

Traditional toxicological studies usually depict the dose-effect relationship at the given exposure time while ignore how toxic effects vary during and after the exposure. In the present study, feeding depression of the rotifer Brachionus calyciflorus under the impact of dimethoate was evaluated not only in the exposure time but also in the post-exposure time. Both the filtration rates (F) and the ingestion rates (I) of the rotifers varied during the exposure period under any concentration of dimethoate. A three-stepwise stress was observed when the rotifers were exposed to 0.2 and 1.0 mg L(-1) of the pesticide respectively, whereas 1.8 mg L(-1) dimethoate caused only one-step stress on the rotifers during the whole period. In addition, although the feeding behaviors of the rotifers recovered after the exposure, two feeding rates both got even more depressed at 8h in the post-exposure time. The latent inhibition after exposure was related to the exposure concentrations and the exposure time. Our works indicated that the impact of dimethoate on the feeding behavior of the rotifers was highly process-dependent that caused the stepwise stress during the exposure period and the latent stress after the exposure.

Integrated toxic evaluation of sulfamethazine on zebrafish: Including two lifespan stages (embryo-larval and adult) and three exposure periods (exposure, post-exposure and re-exposure)

Persistence of antibiotics in aquatic environment may pose a risk to the non-target aquatic organisms. This study provided an integrated evaluation to analyze the toxic stress of sulfamethazine (SMZ) on zebrafish in two lifespan stages (embryo-larval and adult) and three exposure periods (exposure, post-exposure and re-exposure). Zebrafish embryos and adult zebrafish were exposed to SMZ at 0.2, 20 and 2000 μg/L, respectively. The results showed that SMZ at any given concentration inhibited the hatching of embryos at 58-96 hpf (hours post-fertilization). Our result also indicated that two major kinds of the malformation, which was induced by the antibiotic, were edema and spinal curvature. Additionally, the antibiotic stimulated the heartbeat while reduced the body length of the embryo at 72 hpf. Superoxide dismutase (SOD) activities and malondialdehyde (MDA) contents significantly increased at 120 hpf when the embryos were exposed to the lowest concentration (0.2 μg/L) of the antibiotic. On the other hand, the antibiotic induced SOD activities and MDA contents in adult zebrafish in the exposure and re-exposure periods. The MDA contents could recover while SOD activities still increased in 2 d after the exposure. Both SOD activities and MDA contents could recover in 7 d after the exposure. Levels of SOD and MDA in the re-exposure were higher than those in the first exposure. Our results suggested that SMZ had toxic effects on both embryos and adult zebrafish, and provided an integrated evaluation of the toxic effects of SMZ on zebrafish at a new perspective.

An integrated assessment of ceftazidime and photoproducts on the feeding behavior of rotifers: From exposure to post-exposure

Compared to traditional toxicological studies, which depict the dose-effect of contaminants themselves on organisms at the given time, the exposure and post-exposure impacts of antibiotic ceftazidime and its photoproducts are carried out to systematically evaluate the environmental risk fate of ceftazidime in aquatic environments. For the exposure process, the promotion effect of ceftazidime on the feeding behavior of the rotifers decreased when the target compound was irradiated by sunlight, and the promotion effect was converted into inhibition effect, which indicated that the highest toxicity of ceftazidime on the feeding behavior of the rotifers was found after UV-B irradiation. The overcompensation occurred in the post-exposure, indicating a short - term effect of the corresponding photoproducts on the rotifer. In order to better understand the mechanism of this change, the photodegradation pathways of the target compound was analyzed and compared. The degradation degree under the UV-B irradiation had intensified greatly than that under the nature light irradiation. The reactive oxygen species (ROS) of the rotifer in exposure and post-exposure was also detected. Ceftazidime and photoproducts induced generation of ROS, indicating that oxidative damage occurred, and the decreasing of ROS levels could be viewed as the recovery of the rotifers in the post-exposure.

The impact assessment of anticancer drug imatinib on the feeding behavior of rotifers with an integrated perspective: Exposure, post-exposure and re-exposure

The anticancer drugs are getting increasing attention as an emerging contaminant in the aquatic environments. In the present study, feeding behavior of the rotifer Brachionus calyciflorus under the impact of anticancer drug imatinib was evaluated. Traditional toxicological studies usually focus on dose-effect relationship at a given exposure time, while ignore the possible impact after the exposure. Thus, how the impact varied in the post-exposure and re-exposure was also considered in the present study. The feeding depression of the rotifers was attributed to the increased concentration of imatinib. Although the filtration and ingestion rate of the rotifers recovered to a certain extent after the exposure, the significant feeding inhibition still persisted even if the exposure was ended. In the re-exposure period, the feeding behavior was less depressed than those of the exposure period, which implied that rotifers might develop a tolerance to the same toxics. The activities of acetylcholine esterase (AchE) and the levels of reactive oxygen species (ROS) in rotifers were also detected. Imatinib inhibited the activities of AchE in the exposure and re-exposure while ROS levels increased significantly in the re-exposure period. Our present study provided an integrated assessment the potential environmental risks of imatinib at a new perspective.

Investigation of the removal mechanism of antibiotic ceftazidime by green algae and subsequent microbic impact assessment

The present study provides an integrated view of algal removal of the antibiotic ceftazidime and its basic parent structure 7-aminocephalosporanic acid (7-ACA), including contribution analysis, bacteriostatic and aquatic toxic assessment and metabolite verification. 92.70% and 96.07% of the two target compounds was removed after the algal treatment, respectively. The algal removal can be separated into three steps: a rapid adsorption, a slow cell wall-transmission and the final biodegradation. Additionally, while ceftazidime demonstrated an excellent inhibitory effect on Escherichia coli, there was no bacteriostasis introduced after the algal treatment, which could avoid favoring the harmful selective pressure. On the other hand, no significant aquatic impact of the two target compounds on rotifers was observed and it was not enhanced after the algal treatment. To better reveal the mechanism involved, metabolite analyses were performed. Δ-3 ceftazidime and trans-ceftazidime were regarded as the metabolites of ceftazidime and the metabolite of 7-ACA was regarded as a compound which shared the similar structure with 4-chlorocinnamic acid. Our study indicated that the green algae performed a satisfactory growth capacity and played a dominant role for the biodegradation of the target antibiotics, which achieved high removal efficiency and low environmental impact.

Occurrence and partitioning behavior of organophosphate esters in surface water and sediment of a shallow Chinese freshwater lake (Taihu Lake): Implication for eco-toxicity risk

Organophosphate esters (OPEs) are ubiquitous in the aquatic environment, which have been considered or suspected as carcinogens and neurotoxicants. In this study, the occurrence, spatial distribution, potential sources, partitioning character and potential risks of OPEs in the surface water and sediment collected from Taihu Lake were investigated. The concentrations of ∑12 OPEs varied from 1.0 × 102 to 1.7 × 103 ng/L for the surface water and from 8.1 to 4.2 × 102 ng/g dw for the sediment. Trimethyl phosphate (TEP) was the predominant congener in the surface water, while Tris(2-ethylhexyl) phosphate (TEHP) in the sediment. Positive correlations between OPEs indicated that they may have the same sources and/or similar environmental behavior. The pseudo-partitioning values of OPEs ranged from 0.59 to 6.5 × 104 L/kg. TEHP has the highest pseudo-partitioning coefficient, which indicated that TEHP inclined to be enriched in the sediment in Taihu Lake. Risk assessment (RQ) showed that individual OPEs in the surface water and sediment posed no/low risk to aquatic organisms, except 2-Ethylhexyl diphenyl phosphate (EHDPP) (moderate risk) in water.

The dual function of the algal treatment: Antibiotic elimination combined with CO2 fixation

The study provided an algal treatment to achieve dual function with antibiotic elimination and CO2 fixation simultaneously. Two widely used antibiotics, cefradine and amoxicillin were selected as the target compounds. First of all, we assessed the influence of light intensity on algal growth and antibiotic removal efficiency to obtain the optimal light intensity. Secondly, after the algal antibiotic treatment, the CO2 capture capacities at varied CO2 volume concentrations were assessed and compared. Significant improvement in the removal efficiency of cefradine occurred when CO2 was added into the treatment. Change in the content of photosynthetic pigments and the activities of RuBisCO and carbonic anhydrase occurred as the algal responses to the treatment condition. Our results showed that Chlorella pyrenoidosa performed better than Microcystis aeruginosa in both the antibiotic removal efficiency and the CO2 capture capacity. In the integrated algal treatment, the remove rate of antibiotic has been improved by 30.16% and at the same time, the CO2 absorption rate has been promoted by 10.94%. Metabolite analyses also revealed the mechanism involved, which proved the crucial role of the algae in the biodegradation of the target antibiotic.