Ruşen Dündaröz

Analysis of DNA Damage Using the Comet Assay in Infants Fed Cow’s Milk

It has been hypothesized that non-human milk feeding may increase the risk for cancer or for a specific cancer or group of cancers as well as the risk for diseases such as type-1 diabetes mellitus and Crohn’s disease. Regarding DNA damage leading to cancer development in the absence of human milk protection, a comparison between infants fed human milk and cow’s milk has been performed. Each group consisted of 35 infants, whose ages ranged from 9 to 12 months. The level of DNA damage in the peripheral blood lymphocytes of infants has been studied by the comet assay. A significant increase has been found in the number of limited DNA-damaged (p < 0.001) and extensive DNA-damaged (p < 0.001) cells of infants fed cow’s milk. To our knowledge, this is the first study using the comet assay on infants not breast-fed. Supporting our previous SCE study, these results suggest that there is some level of DNA damage in the lymphocytes of infants not breast-fed and this may lead to malignancy in childhood or later in life.

Evaluation of DNA damage using the comet assay in children on long-term benzathine penicillin for secondary prophylaxis of rheumatic fever

Abstract Backround : Benzathine penicillin is the most widely used antibiotic in the prophylaxis of children with rheumatic fever. The aim of the present study was to evaluate the DNA damage in children receiving one dose of 1.2 million units benzathine penicillin every 4 weeks over a long period to prevent recurrences of rheumatic fever.

Intracavernous application of diazepam: an alternative route of the seizure treatment--an experimental study in rabbits.

Backround : There is a general need to terminate seizures as soon as possible using anticonvulsant drugs via an intravenous (i.v.) route, but it is often difficult to achieve a secure i.v. line during the seizure, especially in children. However, it has been demonstrated that high volumes of fluid can be injected into the corpora cavernosa. The purpose of this study was to evaluate the absorption properties of diazepam (DZ) after intracavernous (i.c.) administration and whether therapeutically significant plasma concentrations can be obtained or not.

Fenobarbital Uygulamasında Intravenöz ve Intraosseöz Yolların Karşılaştırılması

SuleymanDemirel Universitesi TIP FAKULTESI DERGISI: 1999 Aralik; 6(4) Fenobarbital Uygulamasinda Intravenoz ve Intraosseoz Yollarin Karsilastirilmasi Rusen Dundaroz, Turner Turkbay, Ali Sizlan, Tuncer Degim, Faysal Gok, Tahir Ozisik Ozet Status epileptikus klasik olarak 30 dakikadan uzun suren jenaralize tonik-klonik nobet olarak tarif edilir. Uzun suren nobet aktivitesi irreversibl serebral hasar ile sonuclanabilir. Ustelik, nobet aktivitesinin uzun surmesinin kontrol edilebilirligini azalttigi yonunde kanitlar vardir. Damar yolunun saglanmasi nobet esnasinda siklikla zordur. Ote yandan intraosseoz yol (IO) kullanima hazirdir. Bu ca¬lismada, 20 mg/kg dozunda fenobarbital IV ve IO yoldan tavsanlara uygulandi. Fenobarbital kan duzey¬leri olculdu ve sonuclar karsilastirildi. Fenobarbital plazma profilinin IO uygulamada terapotik seviyede oldugu goruldu. IO yolun fenobarbital verilmesinde IV yola alternatif olabilecegi sonucuna varildi. Anahtar Kelimeler: Fenobarbital, kemik iligi infuzyonu, intraosseoz infuzyon, intravaskuler giris, status epileptikus Abstract Comparison of Intravenous and Intraosseous Routes for The Administration of Phenobarbital Status epilepticus (SE) is classically defined as a generalized tonic-clonic seizure lasting longer than 30 min. Prolonged seizure activity can result in irreversible cerebral injury. In addition, evidence suggests that the longer the duration of the seizure, the less likely the activity is to be controlled. IV access, however, is frequently difficult to achieve during the seizure. On the other hand, intraosseous (IO) access is available. In this study, phenobarbital was administered to rabbits using both IV and IO lines at the dose of 20 mg/kg. The levels of phenobarbital in the blood were measured and the results were compared. The plasma profile of phenobarbital was found to be in the therapeutic level when it was administered by IO route. It was concluded that the IO line appeared to be an alternative route to IV access in the administration of phenobarbital. Key Words: Phenobarbital, bone marrow infusion, intraosseous infusion, intravascular access, status epilepticus

Administration of Anticonvulsant Drugs by the Intraperitoneal Route

SuleymanDemirel Universitesi TIP FAKULTESI DERGISI: 1999 Eylul; 6(3) Administration ofAnticonvulsant Drugs by the Intraperitoneal Route Rusen Dundaroz, Tuncer Degim, Ali Sizlan, Mehmet Yasar, Metin Denli, Ugur Tamer Abstract Status epilepticus or seizures cause serious brain damages and irreversible cerebral injury if it prolongs and it is essential to terminate seizure rapidly. Antiepileptic drugs can be introduced to the patients intravenously (IV), hut it is almost impossible to achieve IV access during the seizure. Intraperitoneal (IP) route can be accessible but, IP route of administration of antiepileptic drugs has not been used so far. Therefore the possibility of IP administration of anti epileptic drugs was investigated in this study. Antiepileptic drug (diazepam, phenytoin or phenobarbital) was introduced rabbits and dogs by IV and IP routes and blood samples were collected by the time during the period of 60 to 120 minutes. Blood samples vere analyzed and drug contents -were determined. The plasma profiles of antiepileptic drugs were obtained and compared. The plasma profiles of antiepileptic drugs were found to be low when they were administered by IP route. It was concluded that the IP administration of antiepileptic drugs could not be an alternative route to IV administration. Key Words: Convulsion, anticonvulsant drugs, Intraperitoneal route Antikonvulzon Ilaclarin Intraperitonal Yoldan Verilmeleri Ozet Epilepsi veya epilepsi nobeti ciddi beyin hasarlarina neden olabilir, ve eger fazla uzun surerse geri donussuz beyin harabiyetine neden olabilir, bu nedenle nobetin suratle durdurulmasi oldukca onemlidir. Antiepileptik ilaclar hastalara intravenoz(IV) yolla verilebilir ancak nobetler esnasinda hastaya IV ulasim hemen hemen imkansizdir ancak intraperitonal(IP)yol ile ilac verilmesi mumkundur.