Ruta Jasaityte

Current State of Three-Dimensional Myocardial Strain Estimation Using Echocardiography

With the developments in ultrasound transducer technology and both hardware and software computing, real-time volumetric imaging has become widely available, accompanied by various methods of assessing three-dimensional (3D) myocardial strain, often referred to as 3D speckle-tracking echocardiographic methods. Indeed, these methods should provide cardiologists with a better view of regional myocardial mechanics, which might be important for diagnosis, prognosis, and therapy. However, currently available 3D speckle-tracking echocardiographic methods are based on different algorithms, which introduce substantial differences between them and make them not interchangeable with each other. Therefore, it is critical that each 3D speckle-tracking echocardiographic method is validated individually before being introduced into clinical practice. In this review, the authors discuss differences and similarities of the currently available 3D strain estimation approaches and provide an overview of the current status of their validation.

Multi-Transmit Beam Forming for Fast Cardiac Imaging—Experimental Validation and In Vivo Application

High frame rate (HFR) echocardiography may be of benefit for functional analysis of the heart. In current clinical equipment, HFR is obtained using multi-line acquisition (MLA) which typically requires broadening of transmit beams. As this may result in a significant degradation of spatial resolution and signal-to-noise ratio (SNR), the capacity of MLA to obtain high quality HFR images remains limited. As an alternative, we have demonstrated by computer simulation that simultaneously transmitting multiple focused beams into different directions [multi-line transmit (MLT)], can increase the frame rate without significantly compromising the spatial resolution or SNR. This study aimed to experimentally verify these theoretical predictions both in vitro and in vivo to demonstrate, for the first time, that cardiac MLT imaging is feasible. Hereto, the ultrasound advanced open platform, equipped with a 2.0 MHz phased array, was programmed to interleave MLT and conventional single line transmit (SLT) beam forming. Using these two beam forming methods, images of phantoms and healthy volunteers were acquired and investigated both qualitatively and quantitatively. The results confirmed our simulations that image quality of a 4MLT imaging system with a Tukey apodization scheme is very competitive to that of SLT while providing a 4 times higher frame rate. It is also demonstrated that MLT can be combined with MLA to provide images at 12-to 16-fold frame rate (about 340-450 Hz) without significantly compromising spatial resolution and SNR. This is thus the first study to demonstrate that this new ultrasound imaging paradigm is viable which could have significant impact on future cardiac ultrasound systems.

The Heart in Friedreich Ataxia Definition of Cardiomyopathy, Disease Severity, and Correlation With Neurological Symptoms

Background— This cross-sectional study provides a practical approach for the clinical assessment of Friedreich ataxia (FA) cardiomyopathy (FA-CM). Methods and Results— A comprehensive cardiac assessment, including standard echocardiography, color Doppler myocardial imaging, cardiac magnetic resonance imaging, ECG, and exercise stress testing, was performed in 205 FA patients. To assess myocardial hypertrophy in FA-CM, the end-diastolic interventricular septal wall thickness (IVSTd) was found to be the best echocardiographic parameter compared with cardiac magnetic resonance imaging–determined left ventricular mass. With the use of this parameter, 4 groups of patients with FA-CM could be defined. Patients with normal values for IVSTd (31.7%) were classified as having no FA-CM. Patients with an IVSTd exceeding the predicted normal IVSTd were classified as having mild FA-CM (40%) if IVSTd exceeded the normal value by <18% or as having intermediate FA-CM (16.1%) if IVSTd exceeded the normal value by ≥18%. Patients with ejection fraction <50% were classified as having severe FA-CM (12.2%). In addition to increased myocardial mass, severe FA-CM was further characterized by dilatation of the left ventricle, reduced systolic strain rate of the posterior wall, and ECG abnormalities. Regional myocardial function correlated negatively with FA-CM groups. Younger patients had a tendency for more advanced FA-CM. Importantly, no clear correlation was found between FA-CM groups and neurological function. Conclusions— We provide and describe a readily applicable clinical grouping of the cardiomyopathy associated with FA based on echocardiographic IVSTd and ejection fraction data. Because no distinct interrelations between FA-CM and neurological status could be determined, regular follow-up of potential cardiac involvement in FA patients is essential in clinical practice. Clinical Trial Registration— https://www.clinicaltrials.gov. Unique identifier: NCT00905268.Background— This cross-sectional study provides a practical approach for the clinical assessment of Friedreich ataxia (FA) cardiomyopathy (FA-CM). Methods and Results— A comprehensive cardiac assessment, including standard echocardiography, color Doppler myocardial imaging, cardiac magnetic resonance imaging, ECG, and exercise stress testing, was performed in 205 FA patients. To assess myocardial hypertrophy in FA-CM, the end-diastolic interventricular septal wall thickness (IVSTd) was found to be the best echocardiographic parameter compared with cardiac magnetic resonance imaging–determined left ventricular mass. With the use of this parameter, 4 groups of patients with FA-CM could be defined. Patients with normal values for IVSTd (31.7%) were classified as having no FA-CM. Patients with an IVSTd exceeding the predicted normal IVSTd were classified as having mild FA-CM (40%) if IVSTd exceeded the normal value by <18% or as having intermediate FA-CM (16.1%) if IVSTd exceeded the normal value by ≥18%. Patients with ejection fraction <50% were classified as having severe FA-CM (12.2%). In addition to increased myocardial mass, severe FA-CM was further characterized by dilatation of the left ventricle, reduced systolic strain rate of the posterior wall, and ECG abnormalities. Regional myocardial function correlated negatively with FA-CM groups. Younger patients had a tendency for more advanced FA-CM. Importantly, no clear correlation was found between FA-CM groups and neurological function. Conclusions— We provide and describe a readily applicable clinical grouping of the cardiomyopathy associated with FA based on echocardiographic IVSTd and ejection fraction data. Because no distinct interrelations between FA-CM and neurological status could be determined, regular follow-up of potential cardiac involvement in FA patients is essential in clinical practice. Clinical Trial Registration— [https://www.clinicaltrials.gov][1]. Unique identifier: [NCT00905268][2]. # Clinical Perspective {#article-title-30} [1]: https:www.clinicaltrials.gov [2]: /lookup/external-ref?link_type=CLINTRIALGOV&access_num=NCT00905268&atom=%2Fcirculationaha%2F125%2F13%2F1626.atom

Elastic Image Registration Versus Speckle Tracking for 2-D Myocardial Motion Estimation: A Direct Comparison In Vivo

Despite the availability of multiple solutions for assessing myocardial strain by ultrasound, little is currently known about the relative performance of the different methods. In this study, we sought to contrast two strain estimation techniques directly (speckle tracking and elastic registration) in an in vivo setting by comparing both to a gold standard reference measurement. In five open-chest sheep instrumented with ultrasonic microcrystals, 2-D images were acquired with a GE Vivid7 ultrasound system. Radial (ε_RR), longitudinal (ε_LL), and circumferential strain (ε_CC) were estimated during four inotropic stages: at rest, during esmolol and dobutamine infusion, and during acute ischemia. The correlation of the end-systolic strain values of a well-validated speckle tracking approach and an elastic registration method against sonomicrometry were comparable for ε_LL (<i>r</i>=0.70 versus <i>r</i>=0.61 , respectively; <i>p</i>=0.32) and ε_CC (<i>r</i>=0.73 versus <i>r</i>=0.80 respectively; <i>p</i>=0.31). However, the elastic registration method performed considerably better for ε_RR (<i>r</i>=0.64 versus <i>r</i>=0.85 respectively; <i>p</i>=0.09). Moreover, the bias and limits of agreement with respect to the reference strain estimates were statistically significantly smaller in this direction (<i>p</i> <; 0.001). This could be related to regularization which is imposed during the motion estimation process as opposed to an a posteriori regularization step in the speckle tracking method. Whether one method outperforms the other in detecting dysfunctional regions remains the topic of future research.

The Slope of the Segmental Stretch-Strain Relationship as a Noninvasive Index of LV Inotropy

OBJECTIVES The aim of this study was to test the hypothesis that the noninvasively constructed slope of the relationship between left ventricular (LV) regional systolic strain and stretch during atrial contraction represents LV inotropic state. BACKGROUND LV systolic response to a changing preload depends on its inotropic state. Changing the preload has allowed constructing the slope of the end-systolic pressure-volume relationship that is used as an invasive measurement of LV inotropy. We assumed that the slope of the relationship between regional systolic LV strain (total_S) and stretch during atrial contraction (preS) depends on the LV inotropic state as well and can thus be used as a LV inotropy index. METHODS Strain curves (tissue Doppler) were extracted from 27 healthy individuals to determine the normal stretch-strain relationship at rest, during a low-dose dobutamine (LD) challenge and during passive leg-lift (LL). The method was also applied in 7 patients with breast cancer before and after chemotherapy with anthracyclines. RESULTS PreS and total_S correlated closely in all subjects (r = 0.82). Total_S values increased (p < 0.05) with LD (-20.44 ± 3.89% vs. -24.24 ± 5.55%) and LL (-19.65 ± 3.77% vs. -24.05 ± 3.67%), whereas preS increased only with LL (5.96 ± 1.72% vs. 8.61 ± 2.18%), but not with LD (6.83 ± 2.34% vs. 7.29 ± 2.24%). No changes of total_S or preS were observed after the exposure to chemotherapy (-21.23 ± 2.93% vs. -21.49 ± 2.89% and 8.11 ± 1.03% vs. 8.59 ± 1.73%, respectively). The slope of stretch-strain relationship got steeper with LD (-1.47 ± 0.36 vs. -2.34 ± 0.36, p < 0.05), declined after the chemotherapy (-1.68 ± 0.15 to -0.86 ± 0.23, p < 0.05) and did not change with LL (-1.39 ± 0.57 vs. -1.51 ± 0.38, p = NS). CONCLUSIONS The slope of the regional stretch-strain relationship can be regarded as a noninvasive index of myocardial inotropic state. It gets steeper with increasing inotropy, does not change with preload induced changes of LV systolic function, and flattens after the exposure to a cardiotoxic drug.

Whole myocardium tracking in 2D-echocardiography in multiple orientations using a motion constrained level-set

The segmentation and tracking of the myocardium in echocardiographic sequences is an important task for the diagnosis of heart disease. This task is difficult due to the inherent problems of echographic images (i.e. low contrast, speckle noise, signal dropout, presence of shadows). In this article, we extend a level-set method recently proposed in Dietenbeck et al. (2012) in order to track the whole myocardium in echocardiographic sequences. To this end, we enforce temporal coherence by adding a new motion prior energy to the existing framework. This motion prior term is expressed as new constraint that enforces the conservation of the levels of the implicit function along the image sequence. Moreover, the robustness of the proposed method is improved by adjusting the associated hyperparameters in a spatially adaptive way, using the available strong a priori about the echocardiographic regions to be segmented. The accuracy and robustness of the proposed method is evaluated by comparing the obtained segmentation with experts references and to another state-of-the-art method on a dataset of 15 sequences (≃ 900 images) acquired in three echocardiographic views. We show that the algorithm provides results that are consistent with the inter-observer variability and outperforms the state-of-the-art method. We also carry out a complete study on the influence of the parameters settings. The obtained results demonstrate the stability of our method according to those values.

Consistent Regional Heterogeneity of Passive Diastolic Stretch and Systolic Deformation in the Healthy Heart: Age-Related Changes in Left Ventricle Contractility

The consistency of the normal spatial distribution of segmental passive stretch (PreS) and systolic strain (SS) within the left ventricle was investigated and a recently proposed echocardiographic estimate of left ventricular (LV) contractility was used to detect contractility changes with age. Hereto, in 54 healthy subjects, segmental PreS and SS were measured on tissue Doppler images of six left ventricle walls. For each subject, a linear regression line was estimated through the segmental PreS and SS values. The slopes and intercepts of this PreS-SS relationship did not differ between age groups, suggesting no changes in LV contractility with age. Moreover, a consistent regional distribution of PreS was observed, with the highest values measured in the septum, resulting in a similar distribution of SS as a direct consequence of the Frank-Starling mechanism.

Multiview myocardial tracking in echocardiographic 2D sequences using shape and motion constrained level-set

Segmentation of the myocardium in echocardiographic images is an important task for the diagnosis of heart disease. This task is difficult due to the inherent problems of echographic images (i.e. low contrast, speckle noise, signal dropout, presence of shadows). In this article, we extend a level-set method recently proposed in [1] in order to track the whole myocardium in echocardiographic sequences. To this end, we formulate a new motion prior energy that constrains the zero-level of the implicit function to satisfy the optical flow hypothesis. The algorithm is then compared to experts references and to another method on a dataset of 12 sequences (more than 700 images) acquired in the four main echocardiographic views.

Three-dimensional myocardial strain estimation from volumetric ultrasound: Experimental validation in an animal model

Although real-time three-dimensional echocardiography has the potential to allow for more accurate assessment of global and regional ventricular dynamics compared to the more traditional two-dimensional ultrasound examinations, it still requires rigorous testing and validation against other accepted techniques should it breakthrough as a standard examination in routine clinical practice. Very few studies have looked at a validation of regional functional indices in an in-vivo context. The aim of the present study therefore was to validate a previously proposed 3D strain estimation-method based on elastic registration of subsequent volumes on a segmental level in an animal model. Volumetric images were acquired with a GE Vivid7 ultrasound system in five open-chest sheep instrumented with ultrasonic microcrystals. Radial (εRR), longitudinal (εLL) and circumferential strain (εCC) were estimated during four stages: at rest, during esmolol and dobutamine infusion, and during acute ischemia. Moderate correlations for εLL (r=0.63; p<;0.01) and εCC (r=0.60; p=0.01) were obtained, whereas no significant radial correlation was found. These findings are comparable to the performance of the current state-of-the-art commercial 3D speckle tracking methods.

Motion and deformation estimation of cardiac ultrasound sequences using an anatomical B-spline transformation model

We present a novel method for tracking myocardial motion in 2D ultrasound sequences based on non-rigid registration using an anatomical free-form deformation (AFFD) model where the basis functions are locally oriented along the radial and circumferential direction of the left ventricle (LV). This formulation allows us to model the LV motion more naturally compared to previously proposed FFDs defined on a regular Cartesian grid (CFFD). In this paper we compare the performance of the AFFD against the CFFD model in an in-vivo setting. Short-axis images were acquired in five open-chest sheep using sonomicrometry as ground-truth deformation estimates. We demonstrated that regional end-systolic strain values assessed with the AFFD model are comparable with CFFD, while also displaying a statistically lower drift at the end of the cardiac cycle and a better agreement with a manual end-systolic reference. Furthermore, tracking using AFFD was visually more appealing to clinical experts.