Ryo Miyake

Cell measurement by using a flow cytometry chip with a twisted micro-sheath flow channel

We have developed an integrated flow cytometry chip, which comprises a pre-treatment part and a sheath flow-forming part with a twisted microchannel structure, for continuous monitoring of bacteria in drinking water. Due to its unique elastic properties, polydimethylsiloxane (PDMS) was used to fabricate the twisted microchannel that forms the sheath flow, which is a three-dimensional focusing flow. Cells from a human lung cancer cell line were stained in the pre-treatment part and the fluorescence of the stained cells was successfully detected at the sheath flow-forming part. With no dead volume between the two parts, the transfer time was nearly zero, and only 30 μl of sample fluid and approximately 6 μl of reagent were required.

Application of Hybrid Petri Nets for a Drawing Blood Flow from Fingertip.

Our objective of this study are to make a fluid dynamical model and to conduct the flow simulation for obtaining a large amount of drawing blood from a fingertip. The processes of drawing blood are hybrid systems including both the continuity system of blood flow and the discrete systems of cuff pressing and puncture. Therefore, we made the modelling of the fingertip drwaing blood process from the analogy of fluid daynamic tank and pipe systems control using hybrid Petri nets. Using the hybrid Petri nets simulation with cuff pressing and puncture modeled as discrete and blood flow modeled as continuity, we confirmed that the simulation results were agreement with the experimental drawing blood data.

Influence of complex permittivity on confocal imaging of 3D-printed breast phantom

Confocal imaging of a breast tumor phantom embedded in a 3D-printed breast phantom was demonstrated. 16 compact ultra-wideband (UWB) slot antennas were attached on the surface of the 3D-printed breast phantom and confocal imaging of 192 combinations was carried out by a radar-based breast cancer detection system. The breast adipose, glandular and tumor tissues were made of acrylonitrile butadien styrene plastic, the mixture of glycerin and water (saline) and bacon with the size of 10 mm × 10 mm × 10 mm.

Airborne virus detection by a sensing system using a disposable integrated impaction device.

There are many respiratory infections such as influenza that cause epidemics. These respiratory infection epidemics can be effectively prevented by determining the presence or absence of infections in patients using frequent tests. We think that self-diagnosis may be possible using a system that can collect and detect biological aerosol particles in the patients breath because breath sampling is easy work requiring no examiner. In this paper, we report a sensing system for biological aerosol particles (SSBAP) with a disposable device. Using the system and the device, someone with no medical knowledge or skills can safely, easily, and rapidly detect infectious biological aerosol particles. The disposable device, which is the core of the SSBAP, can be an impactor for biological aerosol particles, a flow-cell for reagents, and an optical window for the fluorescent detection of collected particles. Furthermore, to detect the fluorescence of very small collected particles, this disposable device is covered with a light-blocking film that lets only fluorescence of particles pass through a fluorescence detector of the SSBAP. The SSBAP using the device can automatically detect biological aerosol particles by the following process: collecting biological aerosol particles from a patients breath in a sampling bag by the impaction method, labeling the collected biological aerosol particles with fluorescent dyes by the antigen-antibody reaction, removing free fluorescent dyes, and detecting the fluorescence of the biological aerosol particles. The collection efficiency of the device for microspheres aerosolized in the sampling bag was more than 97%, and the SSBAP with the device could detect more than 8.3  ×  10(3) particles l(-1) of aerosolized influenza virus particles within 10 min.

Micro cell isolation column for allergic diagnosis

We suggest a new micro cell isolation column of basophils for an allergic diagnostic system for detecting human basophils activations. Surface plasmon resonance imaging (SPRI) biosensors using human basophils allow allergic diagnosis of less than 1 ml of peripheral blood. However, an isolation of basophils from a small amount of blood is not easy. In this study, we constructed a new micro cell isolation column for basophils with poly(dimethylsiloxane) (PDMS) microflow pass including magnetic particles. Furthermore, we determined whether leukocytes were captured by the micro cell isolation column from a small amount of blood. We can isolate basophils from other leukocytes by using the micro cell isolation column.

Confocal imaging of breast tumor phantom using 3-D-printed breast phantom

Confocal imaging of a breast tumor phantom embedded in a 3-D-printed breast phantom was demonstrated. 16 compact ultra-wideband (UWB) slot antennas were attached on the surface of the 3-D-printed breast phantom and confocal imaging of 192 combinations was carried out by a radar-based breast cancer detection system. The breast adipose, glandular and tumor tissues were made of acrylonitrile butadien styrene plastic, silicone and cured pork meat, respectively. The three dimensional confocal image of the tumor phantom at the position of x=55 mm, y=55 mm, z=30 mm was reconstructed.