Ryoichi Hirayama
Chiba University
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Featured researches published by Ryoichi Hirayama.
RAD Conference | 2017
Masatsugu Ohgami; Nobuhiko Takai; Masahiko Watanabe; Koichi Ando; Akiko Uzawa; Ryoichi Hirayama
The intestinal crypt stem cells in gut have a high growth potential and radiosensitivity, it is dose-dependently reduced by heavy-ion irradiation and intestinal death occurs by arrest of epithelial cells supply in high dose area. The radiation to abdominal cancer, for example uterus and bladder, could give impairments not only on tumor, but also on gut nearby target. Therefore, the development of radioprotective agents for gut may contribute to more effective and less harmful heavy-ion therapy. N-methyl-D-aspartate receptor (NMDAR) is one of glutamate receptors and NMDAR antagonist has been reported to prevent the radiation-induced injuries in the central nervous system. Thus, we examined whether the peripheral NMDAR activation is a possible cause of gut injuries in mice irradiated with carbon-ion beam. We compared the dose-dependent change in the number of crypts after irradiation between treated MK-801 (0.1 mg/kg), a noncompetitive NMDAR antagonist, and untreated mice in order to confirm a MK-801 radioprotective effect on crypts. Compared with the sham group, the number of crypts in MK-801 group was significantly increased at 12.0 Gy or over. The radiolabeled [3H]MK-801 was intravenously injected with C3H female mice received 9 Gy whole body irradiation (290 MeV/u, 20 keV/uf06dm). The significant increase was observed in [3H]MK-801 at 24 hr and 48 hr after irradiation, followed by decrease thereafter. These results suggest that intestinal NMDAR are most activated at 48 hr after carbon-ion irradiation. Thus, we suggested that radiation-induced gut injuries could be suppressed by NMDAR antagonists as radioprotective agents until 48 hr after carbon-ion exposure.
RAD Conference | 2017
Nobuhiko Takai; Masatsugu Ohgami; Koichi Ando; Akiko Uzawa; Ryoichi Hirayama; Saori Nakamura; Yoshihito Ohba
Cerebral dysfunction is one of the major concerns associated with radiotherapy of brain tumours. However, little is known about the neurochemical basis of brain dysfunction induced by proton irradiation. We here investigated the early consequences of brain damages caused by a proton beam. Brains of male wistar rats were locally irradiated with a 70 MeV proton beam. The irradiation dose was set at level known to produce vascular change followed by necrosis, which appeared the late period after irradiation with 30 Gy. The eight-arm radial maze task in irradiated rats was used. In order to assess the preservation (recall) of memory, the rats that showed the spatial cognition were irradiated. The impairment of the preservation memory was not observed in the irradiated rats compared to the control ones 24 hrs after irradiation. Repeated measures of two-way ANOVA of correct choices and number of errors showed no differences between the control group and 30 Gy irradiated group. In order to assess the acquisition process of memory and working memory for the platform location in the water maze, the task was started on the 24 hrs after irradiation. In the learning task (the acquisition process of memory), there was no difference between the control group and irradiated group in the latency to platform. The rats that memorized the location of the standard position were irradiated, and the impairment of the long-term memory was not observed in the irradiated rats compared to the control ones 24 hrs after irradiation. However, the irradiated rats required a substantially longer time finding out the platform than the control rats when the platform was placed in a non-standard position. From this it follows that a proton dose of 30 Gy impaired the working memory of rats. The function of muscarinic acetylcholine receptors was analyzed by in vivo binding assay using radioligand quinuclidinyl benzilate ([3H]QNB). The irradiated rats were intravenously injected with 5.5 MBq of [3H]QNB on the 24 hrs after the irradiation. Autoradiographic studies showed a transitional increase of [3H]QNB in-vivo binding in the early phase after proton irradiation. On the other hand, no change in in-vitro [3H]QNB binding was seen in the autoradiogram of brain slices from the irradiated rats. The cerebral blood flow and the histopathological change in the brain appeared at 5 or 6 months after irradiation. These results indicate that the relation between behavioral impairment caused by radiation is closely related to the early change in the receptor function which could be detected in in-vivo conditions.
Journal of Radiation Research | 2005
Ryoichi Hirayama; Yoshiya Furusawa; Takeshi Fukawa; Koichi Ando
Archive | 2015
Hideki Matsumoto; Masanori Tomita; Munetoshi Maeda; Masanori Hatashita; Tomoo Funayama; Yuichiro Yokota; Yasuhiko Kobayashi; Ryoichi Hirayama; Teruaki Konishi
Archive | 2014
Chen Dong; Wentzi Tu; Jiamei Fu; Teruaki Konishi; Ryoichi Hirayama; Yukio Uchihori; Yoshiya Furusawa; Chunlin Shao
Archive | 2014
Akiko Uzawa; Ryoichi Hirayama; Yoshitaka Matsumoto; Kana Koda; Sachiko Koike; Koichi Ando; Yoshiya Furusawa
Archive | 2014
Ryuichi Okayasu; Hirokazu Hirakawa; Aya Masaoka; Miho Noguchi; Ryoichi Hirayama; Huizi Li; Yoshitaka Matsumoto; Akira Fujimori
Archive | 2010
Hiroshi Maezawa; Jun Itami; Ryoichi Hirayama; Yoshiya Furusawa
The Japan Radiation Research Society Annual Meeting Abstracts The 52nd Annual Meeting of the Japan Radiation Research Society | 2009
Nobuhiro Takase; Ryoichi Hirayama; Mizuki Ueno; Yoshiya Furusawa; Ryuichi Okayasu; Chieko Murayama; Atsushi Ito
The Japan Radiation Research Society Annual Meeting Abstracts The 52nd Annual Meeting of the Japan Radiation Research Society | 2009
Sachiko Koike; Koichi Ando; Akiko Uzawa; Yoshiya Furusawa; Ryoichi Hirayama; Yoshitaka Matsumoto; Mitsuaki Yoshida; Ryuichi Okayasu