S. A. Mamilov

Laser-optical method of stimulation of cell metabolism and its biomedical applications

The method of direct control of tissue local oxygen concentration based on in vivo laser-induced photodissosiation of oxyhemoglobin is discussed. This method gives unique possibility in selective and local increase of the concentration of free molecular oxygen into tissue that enhances metabolism of cells. Novel method of dosimetry based on key biological parameters connected with aerobic metabolism is discussed. Different biomedical applications of these methods are proposed.

Investigation in vivo the effect of temperature on quantum yield of photodissociation of oxyhemoglobin (HbO/sub 2/) in cutaneous blood vessels

The temperature effect on laser-induced photodissociation of oxyhemoglobin in vivo by measuring changes in arterial blood oxygen saturation is investigated. It is shown that during laser irradiation the local concentration of free oxygen in tissue significantly increases when temperature of the body exceeds 40/spl deg/C compare to normal one. Different biomedical applications of this phenomenon are proposed. Unique possibility in selective and local increase of the concentration of free molecular oxygen in tissue that enhances metabolism of cells is demonstrated. An important conclusion is drawn from the obtained results that temperature dependence of quantum yield of photodissociation of oxyhemoglobin should be taken into account in developing new therapeutic methods for laser medicine.

High sensitive pulse oximeter-spectrophotometer for laser-optical dosimetry in biology and medicine

High sensitive multi channel pulse oxymeter - spectrophotometer for control of the local tissue oxygen saturation is presented. Due to an original method of data storing and processing the accuracy of measurements 3-4 times are increased in compare with similar systems. This system is used for registration of the local changes of arterial blood saturation with oxygen under effect of low intensity laser radiation. It is shown that the photodissociation of oxyhemoglobin in cutaneous blood vessels play a dominant role in the mechanism of biostimulating and therapeutic effect of low intensity laser radiation. The results of experimental investigation in vivo the dependence of arterial oxygen blood saturation on temperature and the wavelength of laser radiation are presented. It is suggested that dosimetry for laser therapy could be base on regulation of local concentration of free oxygen in tissue.

Optical monitoring of laser-engraved flexo plates

The work concerns alternate versions of non-damaging optical monitoring of laser engraved flexo plates including (1) image acquisition of made by Computer-to-Plate technique masks using optics-analogue of flatbed scanners and mobile CCD microscope with calculation of halftone screen parameters and (2) measurement of flexo plate relief formed by direct laser engraving and by CtP technique using laser triangulation profilometry.