S. Vairamani

Structural characterization and bioactivities of sulfated polysaccharide from Monostroma oxyspermum.

Sulfated polysaccharide was isolated from Monostroma oxyspermum through hot water extraction, anion-exchange and gel permeation column chromatography. The sulfated polysaccharide contained 92% of carbohydrate, 0% of protein, 7.8% of uronic acid, 22% of ash and 33% of moisture respectively. The elemental composition was analyzed using CHNS/O analyzer. The molecular weight of sulfated polysaccharide determined through PAGE was found to be as 55 kDa. Monosaccharides analysis revealed that sulfated polysaccharide was composed of rhamnose, fructose, galactose, xylose, and glucose. The structural features of sulfated polysaccharide were analyzed by NMR spectroscopy. Further the sulfated polysaccharide showed total antioxidant and DPPH free radical scavenging activity were as 66.29% at 250 μg/ml and 66.83% at 160 μg/ml respectively. The sulfated polysaccharide also showed ABTS scavenging ability and reducing power were as 83.88% at 125 μg/ml and 15.81% at 400 μg/ml respectively. The anticoagulant activity was determined for human plasma with respect to Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT) was 20.09 IU and 1.79 IU at 25 μg/ml respectively. These results indicated that the sulfated polysaccharide from M. oxyspermum had potent antioxidant and anticoagulant activities.

Structural characterization and biomedical properties of sulfated polysaccharide from the gladius of Sepioteuthis lessoniana (Lesson, 1831).

Sulfated polysaccharide was extracted from the internal shell (gladius) of Sepioteuthis lessoniana. The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively. The elemental composition was analyzed using CHNS/O analyzer. The molecular weight of sulfated polysaccharide determined through PAGE was found to be as 66 kDa. Monosaccharides analysis revealed that sulfated polysaccharide was composed of rhamnose, galactose, xylose and glucose. The structural features of sulfated polysaccharide were analyzed by FT-IR and NMR spectroscopy. Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method. The polysaccharide has showed good antibacterial activity and MIC and MBC have also been evaluated. The anticancer activity was tested against HeLa cell line by MTT assay. The Cytotoxic Concentration (CC50) was observed as 700 μg/ml and the maximum anticancer activity of 62.89% was recorded at 200 μg/ml; whereas, the lowest of 9.87% was observed at 25 μg/ml. In conclusion, the sulfated polysaccharide is an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity.

GLYCOSAMINOGLYCANS FROM MARINE CLAM Meretrix meretrix (LINNE.) ARE AN ANTICOAGULANT

The extracted unfractionated glycosaminoglycans (GAGs) obtained from the marine clam Meretrix meretrix were fractionated by ion-exchange (Amberlite IRA-900 and 120) chromatography. The fractionated sample activity was determined through azure-A by metachromatic activity and agarose gel electrophoresis. The active fractionated sample molecular mass was determined through gradient polyacrylamide gel electrophoresis (PAGE). The structural characterization of low-molecular-weight GAGs was analyzed by Fourier transform infrared (FT-IR) and 1H-nuclear magnetic resonance (NMR) spectroscopy. The Activated partial thromboplastin time (APTT) of fractionated heparin-like glycosminoglycan is 72 IU/mg and it has a molecular mass of 15,000 Da. The disaccharide profiles, such as uronic acid 15.31%, hexosamine 24.61%, and sulfate content 11.7%, were also determined. The results of this study suggest that the GAGs from M. meretrix could be an alternative source of heparin.

Evaluation of antioxidant activities and chemical analysis of sulfated chitosan from Sepia prashadi

The chitin and chitosan of S. prashadi was prepared through demineralization, deproteinzation, deacetylation process and sulfation were carried by chlorosulfonic acid in N,N-dimethylformamide. The sulfate content in chitosan was found to be 18.9%. The carbon, hydrogen and nitrogen composition of the sulfated chitosan were recorded 39.09%, 6.95% and 6.58% respectively. The structural analysis was done by using FT-IR and NMR spectroscopy technique. The DSC curves of sulfated chitosan showed a large endothermic peak resolved with To value of 54.57°C and TP value of 97.46°C. The morphology of sulfated chitin and sulfated chitosan were studied by SEM. The Further in vitro antioxidant activity of sulfated chitosan was screened by scavenging activity of superoxide radical assay, hydroxyl radical scavenging assay, metal-ion chelating effect and reducing power. Its anticoagulant activity was tested for human plasma with respect to Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT). Results prove that sulfated chitosan has potent antioxidant and anticoagulant activity.

Isolation, characterization and molecular weight determination of collagen from marine sponge Spirastrella inconstans (Dendy)

Collagen is a major structural protein of connective tissues. It can be used as a prosthetic biomaterial applicable to artificial skin, tendon ligaments and development collagen implants. In the present study, an attempt was made to isolate and characterize collagen from the marine sponge, Spirastrella inconstans. The total protein content of sponge collagen was relatively high (32%). While determining the molecular weight of crude and purified collagen through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), the crude showed three bands (80, 60 and 59 kDa molecular weight) and purified showed only a single band (58 kDa). The structural properties were analyzed by using fourier transform infra red (FT-IR) spectrum and the stability of collagen was also given the single transition peak in differential scanning calorimetry (DSC). The microstructure of sponge collagen showed highly porous and interconnected scaffolds in scanning electron microscopic (SEM) analysis.

THE ANTICOAGULANT ACTIVITY AND STRUCTURAL CHARACTERIZATION OF FRACTIONATED AND PURIFIED GLYCOSAMINOGLYCANS FROM VENERID CLAM MERETRIX CASTA (CHEMNITZ)

Heparin (heterogeneous anionic polysaccharide), the glycosaminoglycans (GAGs) has been used as an anticoagulant for several years in clinical studies. In the present study, GAGs from Meretrix casta were fractionated by ion exchange chromatography (Amberlite IRA-900). The active fractions were identified by agarose gel electrophoresis and metachromatic assay (confirmatory test) pooled, dialyzed, and purified by barium acetate. The molecular weights of both fractionated and purified GAGs were observed through polyacrylamide gradient gel electrophoresis and its structural characterization relied on Fourier transform-infrared and 1H-NMR spectroscopy. Finally the activated partial thromboplastin time and the disaccharides profile of GAGs were also determined. The outcome of results of this study suggested that the extracted GAGs from M. casta could be an alternative source of heparin.

Antibiotic Efficacy and Characterization of Mangrove Metabolites against UTI Microbes

In vitro antibacterial aptitude of Avicennia marina, A. marina var acutissima, Rhizophora mucronata, and R. annamalayana leaves were evaluated for urinary tract infective bacterial strains. A. marina var. acutissima and R. annamalayana methanolic extracts had diverse phytochemicals and demonstrated lower antibacterial activity at the concentrations of 100 to 250 μg.mL−1 and greater activity at 500 to 1,000 μg.mL−1. The minimum inhibitory concentration and minimum bactericidal concentration intensity of methanolic extracts were at 150 to 375 μg.mL−1. Alkaloids and genstine were noted in TLC fractionation, and their bioactivities were determined in bioautography analysis. Active functional groups OH alcoholic (hydrogen bond), C-H methylene, and C-O- aldehyde were present in both active guided fractions, but R2C=CH2 alkene and N-O nitraso groups were only in A. marina var. acutissima.

Isolation and chemical characteristics of rhamnose enriched polysaccharide from G rateloupia lithophila

The crude polysaccharide was extracted from Grateloupia lithophila through hot-water extraction and deproteinization. Further, fractionated by anion-exchange column using Q-Sepharose and purified by gel-permeation chromatography using Sepharose 4-LB column. The crude and purified polysaccharide contains high carbohydrate (75.7 and 89.7%), ash (18.2 and 3.2%) and moisture (14.8 and 1.3%); the protein and uronic acid were absent. The molecular weight of crude, fractionated and purified polysaccharide was found to be 37 kDa, 29 kDa and 24 kDa. The monosaccharide composition of the crude polysaccharide was found to be having rhamnose (79.82%), fructose (8.38%), galactose (3.95%), xylose (3.31%) and glucose (1.48%); whereas the purified polysaccharide reported higher amount of rhamnose (95.88%), 1.13% of xylose and 2.21% of fructose. The structural elucidation of the purified polysaccharide was conformed as α-l-rhamnose through polarimetry, FT-IR and 1H NMR spectroscopy.

Mucopolysaccharide from cuttlefish: Purification, chemical characterization and bioactive potential

The sulfated mucopolysaccharide (GAG) was isolated from S. pharonis and the carbohydrate and protein content was found to be 62.4% and 3.9%. The disaccharide profile of sulfated GAG composed glucuronic acid, N-acetyl glucosamine and sulfate content by contributing 50.11%, 38.00% and 27.69% respectively. The carbon, hydrogen and nitrogen content of the sulfated GAG showed 14.80%, 1.68% and 2.99% respectively. The molecular weight of sulfated GAG was calculated as 27kDa and the structural characterization was done by Fourier Transform Infrared (FT-IR) and NMR Spectroscopy. The Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT) of sulfated GAG were determined as 91 IU and 39.55 IU at 25μg/ml respectively. Further the sulfated GAG reported the cytotoxic effect (CC50) of 1100μg/ml concentration on Vero cell line. The sulfated GAG reported the anticancer activity against HeLa cell line with an inhibition rate of 18.65%-66.13% at 50-250μg/ml concentration. The sulfated GAG can be considered as a potent anticoagulant and anticancer drug in future.

Exploration of the preventive effect of S. lessoniana liver oil on cardiac markers, hematological patterns and lysosomal hydrolases in isoproterenol-induced myocardial infarction in wistar rats: a novel report

The purpose of this study was to explore the in vivo cardioprotective potency of liver (digestive gland) oil from S. lessoniana on isoproterenol induced myocardial infracted wistar rats. Rats received IPH for 2 successive days (85 mg kg−1 body weight) at 24 h intervals to induce myocardial infarction at the end of the experiment. S. lessoniana liver oil was served orally at a dose of 0.05 mL per day for 45 days, then the serum and heart tissue were analysed for CK and LDH enzyme activity, then haematological, lipid profile changes was examined in the blood, histopathological examination was carried in heart tissue and the activity of β-D-glucuronidase, β-D-acetylglucosaminidase, acid phosphatase and cathepsin-D in the serum lysosomal heart fraction. The results of the present study suggested that the pre-treated animals with squid liver oil prevented isoproterenol-induced haematological changes and heart weight increases. Lysosomal membrane integrity was well protected in rats pre-treated with squid liver oil, as indicated by significantly lowered activities of lysosomal hydrolases in the serum and associated increase in their activity in the lysosomal fraction of the heart. The histopathological examination further confirmed that the cardioprotective potency of squid liver oil.