Sadiye Ayşe Çelik

Phytochemical contents and enzyme inhibitory and antioxidant properties of Anethum graveolens L. (dill) samples cultivated under organic and conventional agricultural conditions.

Inhibitory effect of the n-hexane, dichloromethane, ethyl acetate, and ethanol extracts from Anethum graveolens L. (dill) cultivated under organic (AG-O) and conventional (AG-C) conditions was tested against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase at 200 μg mL⁻¹. Their antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), N,N-dimethyl-p-phenylendiamine (DMPD), and nitric oxide (NO) radical scavenging assays as well as ferric ion-chelation capacity, ferric-(FRAP), and phosphomolybdenum-reducing antioxidant power (PRAP). The phytochemical analyses have been performed on both of the plant samples. GC-MS analysis pointed out that α-phellandrene was the main component in both of the essential oils in varying amounts (47.75% for AG-O and 27.94% for AG-C), while oleic acid was the dominant in the fruit oils of two samples (36.39% for AG-O and 53.87% for AG-C). HPLC analysis showed that both of the extracts contained rosmarinic acid as the major phenolic acid. The extracts inhibited BChE at moderate level, while the ethanol extracts exerted remarkable NO scavenging effect. The results emphasize that cultivation conditions may have effect on bioactivity and phytochemical content on plant samples.

The Essential Oil Compositions of Rosmarinus officinalis L. Cultivated in Konya and Collected from Mersin-Turkey

Purpose: In this research, essential oil (EO) compositions of the dried and fresh aerial parts of Rosmarinus officinalis cultivated in the reearch field in Selcuk University Medicinal and Aromatic Plants Department Area in Konya and collected from Mersin was investigated. Material and Methods: EO was distilled by using Clevenger type apparatus for 3 h and the chemical compositions were detected in GC-MS. Results: Although, the oil yields of the collected rosemary was determined to be 0.4 ml (in fresh) and 0.6 ml (in dried) aerial parts, the yields of the cultivated plants for fresh and dried parts were 0.32 ml (in fresh) and 0.9 ml (in dried), respectively. In this research the differences with respect to composition and components were determined between the cultivated and collected plants. On the other hand, in this study, it was also determined that the EO compositions varied with respect to be fresh or dry of the plant parts. While there were 67 and 55 of EO compositions were observed in the fresh and dried parts of the collected marjoram, respectively; in the cultivated plants there were 46 (in fresh) and 79 (in dried) components detected. Conclusion: In this study, it was observed that the major EO compositions were camphor, 1.8-cineole, borneol, α-pinene, linalool, verbenone, bornyl acetate, limonene and camphene. The objective of the study to detect the differences between the EO compositions varied according to be the plant fresh or dry and collected or cultivated.