Saem Han

Simultaneous determination of luteolin and luteoloside in dandelions using HPLC

The content of the two flavonoids, luteolin and luteoloside, in dandelions was determined by high-performance liquid chromatography. A Nucleosil®100-5C18 column was used for the determination of luteolin and luteoloside concentrations. The mobile phase was a mixture of acetonitrile and water containing 0.1% formic acid (99.9:0.1, v/v). The gradient elution program was 10% to 23% acetonitrile within 60 min at a flow rate of 1.0 mL·min−1. The ultraviolet spectra were recorded at 350 nm for the identification of the two flavonoids. The contents of luteolin and luteoloside were highest in T. officinale flowers (5.817 ± 0.030 mg·g−1) and in T. coreanum leaves (6.205 ± 0.087 mg·g−1), respectively. The total content of luteolin and luteoloside in T. coreanum was about 1.3–2 times higher than that found in T. ohwianum and T. officinale.