Sullim Lee

Identification of anti-cancer active components of Taraxacum coreanum on human gastric cancer AGS cells

Anti-cancer effects were compared amongst Taraxacum coreanum extract, its fractions, and 7 ingredients (β-sitosterol, daucosterol, taraxasteryl acetate, chrysoeriol, diosmetin, luteolin, and luteoloside). Exposure to the ethyl acetate fraction (50 and 100 μg/mL) of T. coreanum extract and luteolin (10 and 50 μM) for 24 h induced the cleavage of poly (ADP-ribose) polymerase (PARP), caspase-3, and caspase-8, in a dose-dependent manner. These findings demonstrate that luteolin is the main active component of T. coreanum extract activating caspases-3 and -8 which contribute to apoptotic cell death.

Simultaneous determination of luteolin and luteoloside in dandelions using HPLC

The content of the two flavonoids, luteolin and luteoloside, in dandelions was determined by high-performance liquid chromatography. A Nucleosil®100-5C18 column was used for the determination of luteolin and luteoloside concentrations. The mobile phase was a mixture of acetonitrile and water containing 0.1% formic acid (99.9:0.1, v/v). The gradient elution program was 10% to 23% acetonitrile within 60 min at a flow rate of 1.0 mL·min−1. The ultraviolet spectra were recorded at 350 nm for the identification of the two flavonoids. The contents of luteolin and luteoloside were highest in T. officinale flowers (5.817 ± 0.030 mg·g−1) and in T. coreanum leaves (6.205 ± 0.087 mg·g−1), respectively. The total content of luteolin and luteoloside in T. coreanum was about 1.3–2 times higher than that found in T. ohwianum and T. officinale.