Sajid Rashid Ahmad

Vulnerability and impact assessment of extreme climatic event: A case study of southern Punjab, Pakistan

Understanding of frequency, severity, damages and adaptation costs of climate extremes is crucial to manage their aftermath. Evaluation of PRECIS RCM modelled data under IPCC scenarios in Southern Punjab reveals that monthly mean temperature is 30°C under A2 scenario, 2.4°C higher than A1B which is 27.6°C in defined time lapses. Monthly mean precipitation under A2 scenario ranges from 12 to 15mm and for A1B scenario it ranges from 15 to 19mm. Frequency modelling of floods and droughts via poisson distribution shows increasing trend in upcoming decades posing serious impacts on agriculture and livestock, food security, water resources, public health and economic status. Cumulative loss projected for frequent floods without adaptation will be in the range of USD 66.8-79.3 billion in time lapse of 40years from 2010 base case. Drought damage function @ 18% for A2 scenario and @ 13.5% for A1B scenario was calculated; drought losses on agriculture and livestock sectors were modelled. Cumulative loss projected for frequent droughts without adaptation under A2 scenario will be in the range of USD 7.5-8.5 billion while under A1B scenario it will be in the range of USD 3.5-4.2 billion for time lapse of 60years from base case 1998-2002. Severity analysis of extreme events shows that situation get worse if adaptations are not only included in the policy but also in the integrated development framework with required allocation of funds. This evaluation also highlights the result of cost benefit analysis, benefits of the adaptation options (mean & worst case) for floods and droughts in Southern Punjab. Additionally the research highlights the role of integrated extreme events impact assessment methodology in performing the vulnerability assessments and to support the adaptation decisions. This paper is an effort to highlight importance of bottom up approaches to deal with climate change.

Application of Bacillus megaterium MCR-8 improved phytoextraction and stress alleviation of nickel in Vinca rosea

ABSTRACT The current research was performed to evaluate the effect of Bacillus megaterium MCR-8 on mitigation of nickel (Ni) stress in Vinca rosea grown on Ni-contaminated soil (50, 100, and 200 mg Ni kg−1 soil). The treated plants exhibited reduced growth, biomass, gas exchange capacity, and chlorophyll (Chl) content under Ni stress. The inoculated plants growing in Ni-contaminated media exhibited relatively higher growth, total soluble protein, and proline contents. Similarly, bacterial inoculation improved the activity of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) under Ni stress. The Ni stress alleviation in inoculated plants was attributed to the reduced level of malondialdehyde (MDA) and hydrogen peroxide (H2O2), enhanced synthesis of protein, proline, phenols, and flavonides in conjunction with improved activity of antioxidant enzymes. The growth-promoting characteristics of microbe such as 1-aminocyclopropane-1-carboxylate deaminase (ACCD) and phosphate solubilization activity, siderophore, and auxin production capability also improved the growth and stress mitigation in inoculated plants. Furthermore, the inoculated plants exhibited higher value for bioconcentration factor (BCF), translocation factor (TF), and resulted in higher loss of Ni content from soil. The current results exhibited the beneficial role of B. megaterium MCR-8 regarding stress alleviation and Ni phytoextraction by V. rosea.

Reservoir characterization of basal sand zone of lower Goru Formation by petrophysical studies of geophysical logs

The lower Indus basin is one of the largest hydrocarbon producing sedimentary basins in Pakistan. It is characterized by the presence of many hydrocarbon-bearing fields including clastic and carbonates proven reservoirs from the Cretaceous to the Eocene age. This study has been carried out in the Sanghar oil field to evaluate the hydrocarbon prospects of basal sand zone of lower Goru Formation of Cretaceous by using complete suite of geophysical logs of different wells. The analytical formation evaluation by using petrophysical studies and neutron-density crossplots unveils that litho-facies mainly comprising of sandstone. The hydrocarbons potentialities of the formation zone have been characterized through various isoparameteric maps such as gross reservoir and net pay thickness, net-to-gross ratio, total and effective porosity, shaliness, and water and hydrocarbons saturation. The evaluated petrophysical studies show that the reservoir has net pay zone of thickness range 5 to 10 m, net-togross ratio range of 0.17 to 0.75, effective porosity range of 07 to 12 %, shaliness range of 27 to 40 % and hydrocarbon saturation range of 12 to 31 %. However, in the net pay zone hydrocarbon saturation reaches up to 95%. The isoparametric charts of petrophysically derived parameters reveal the aerial distribution of hydrocarbons accumulation in basal sand unit of the lower Goru Formation which may be helpful for further exploration.

An assessment of pan-sharpening algorithms for mapping mangrove ecosystems: a hybrid approach

ABSTRACT Mapping mangrove (littoral and swamps) ecosystems is challenging due to the qualitative and quantitative nature of the surrounding water and mudflats. However, accurate assessment of mangroves is required to determine carbon credits. This research study explores five pan-sharpening algorithms with the aim of determining the best algorithm to map mangrove ecosystems from very high resolution satellite images. In this research, a multidimensional evaluation was employed to pinpoint the best algorithm from among five advanced algorithms, i.e. Ehler’s transformation (ET), modified intensity hue saturation (MIHS), wavelet transformation (WT), optimized high pass filter addition (OHPFA), and subtractive resolution merge (SRM). These approaches involve the calculation of spectral root mean square error (RMSE), Sobel filter RMSE and correlation coefficient (r). OHPFA and SRM provided good results during this assessment. Object-based image analysis was incorporated to further assess the best technique between these two approaches for assessing mangrove tree canopy by calculating under and over segmentation. The SRM algorithm provides the best results with a kappa coefficient (κ) of 0.875 and an accuracy of 92.3% when compared with ground data. This research is very useful in various applications such as calculation of crown projection area using high resolution satellite images for estimation of blue carbon in mangrove trees.

Effect of Pseudomonas fluorescens RB4 and Bacillus subtilis 189 on the phytoremediation potential of Catharanthus roseus (L.) in Cu and Pb contaminated soils.

ABSTRACT The remediation of heavy metal-contaminated soils has become a critical issue due to toxic effects of these metals on living organisms. The current research was conducted to study the effect of Pseudomonas fluorescens RB4 and Bacillus subtilis 189 on the growth and phytoremediation potential of Catharanthus roseus in Cu- and Pb-contaminated soils. The bacterial strains exhibited significantly higher level of water-extractable Pb and Cu in Pb, Cu, and Cu+Pb-contaminated. The P. fluorescens RB4 inoculated plants, produced 102%, 48%, and 45% higher fresh weight (FW) in soils contaminated with Cu, Pb, and both elements, respectively, as compared to un-inoculated control plants. Similarly, B. subtilis 189 inoculated plants produced 108%, 43%, and 114% more FW in the presence of Cu, Pb, and both elements. The plants co-cultivated with both bacteria exhibited 121%, 102%, and 177% higher FW, in Cu, Pb, and both elements contaminated soils, as compared to respective un-inoculated control. Co-cultivation of P. fluorescens RB4, B. subtilis 189, and P. fluorescens RB4 + B. subtilis 189 resulted in higher accumulation of Cu and Pb in shoots of the C. roseus grown in contaminated soils as compared to un-inoculated control. Bacterial treatments also improved the translocation and metal bioconcentration factors. The growth and phytoextraction capability of C. roseus was improved by inoculation of P. fluorescens RB4 and B. subtilis 189.

Persistent organic pollutants in Pakistan: Potential threat to ecological integrities in terms of genotoxicity and oxidative stress

ABSTRACT As a consequence of both increasing population and industrialization in agro-economic sector, Pakistan has inevitably been confronted by multicomplex environmental challenges. Owing in part to poor regulatory framework, pollution due to persistent organic pollutants (POPs) has caused serious problems throughout the country. Resultantly, extensive use of POPs is causing vigorous deterioration of environment and human health. The current study addresses: (1) the general information on associated ecological effects and toxicity assessment by meta-analysis for local fauna and flora (2) their respective occurrence in living organisms; and (3) sources and distribution patterns of various POPs classes in environmental compartments of Pakistan. Based on the study, it can be concluded that the environment of Pakistan is highly contaminated with organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), dechlorane plus (DP), and polychlorinated naphthalenes (PCNs), which is further supported with the meta-analysis. Nevertheless, unavailability of environmental quality standards and food safety for POPs render it a forthcoming challenge of multicompartment toxicity exposure. Therefore, strategies must be planned for risk assessment of biologically active POPs, while the POP waste inventory should be elevated, along with the necessary measures to promote appropriate handling and treatment of POP as a matter of prime importance.

Role of Ni-tolerant Bacillus spp. and Althea rosea L. in the phytoremediation of Ni-contaminated soils

ABSTRACT In our current study, four nickel-tolerant (Ni-tolerant) bacterial species viz, Bacillus thuringiensis 002, Bacillus fortis 162, Bacillus subtilis 174, and Bacillus farraginis 354, were screened using Ni-contaminated media. The screened microbes exhibited positive results for synthesis of indole acetic acid (IAA), siderophore production, and phosphate solubilization. The effects of these screened microbes on Ni mobility in the soil, root elongation, plant biomass, and Ni uptake in Althea rosea plants grown in Ni-contaminated soil (200 mg Ni kg−1) were evaluated. Significantly higher value for water-extractable Ni (38 mg kg−1) was observed in case of Ni-amended soils inoculated with B. subtilis 174. Similarly, B. thuringiensis 002, B. fortis 162, and B. subtilis 174 significantly enhanced growth and Ni uptake in A. rosea. The Ni uptake in the shoots and roots of B. subtilis 174-inoculated plants enhanced up to 1.7 and 1.6-fold, respectively, as compared to that in the un-inoculated control. Bacterial inoculation also significantly improved the root and shoot biomass of treated plants. The current study presents a novel approach for bacteria-assisted phytoremediation of Ni-contaminated areas.

Evaluation of state and community/private forests in Punjab, Pakistan using geospatial data and related techniques

BackgroundForests are fundamental in maintaining water supplies, providing economic goods, mitigating climate change, and maintaining biodiversity, thus providing many of the world’s poorest with income, food and medicine. Too often, forested lands are treated as “wastelands” or “free” and are easily cleared for agricultural and infrastructure expansion.MethodsIn this paper, the sustainability of two forest ecosystems (state and community/private owned) was evaluated using SPOT-5 satellite images of 2005 and 2011. This study was conducted in a sub-watershed area covering 468 km2, of which 201 km2 is managed by the state and 267 km2 by community/private ownership in the Murree Galliat region of Punjab Province of Pakistan. A participatory approach was adopted for the delineation and demarcation of forest boundaries. The Geographic Object-Based Image Analysis (GEOBIA) technique was used for identification and mapping of ten Land Cover (LC) features.ResultsThe results show that between the years 2005 to 2011, a total of 55 km2 (24 km2 in state-owned forest and 31 km2 in community/private forest) was converted from forest to non-forest. The conclusion is that conservation is more effective in state-owned forests than in the community/private forests.ConclusionsThese findings may help to mobilize community awareness and identify effective initiatives for improved management of community/private forest land for other regions of Pakistan.

Amelioration of Cadmium Stress in Gladiolus (Gladiolus grandiflora L.) by Application of Potassium and Silicon

ABSTRACT Gladiolus corms were grown in media contaminated with cadmium (Cd) (50 mg kg−1) and supplemented with silicon (Si) and potassium (K). The role of Si and K for mitigation of Cd toxicity was evaluated. Cd-induced stress generated significantly increased level of oxidative stress markers including hydrogen peroxide (H2O2), and malondialdehyde (MDA) in gladiolus. The application of K and Si improved the production of protein and proline in the treated plants. Moreover, K and Si supplemented plants exhibited an improvement in the activity of antioxidant enzymes and a reduction in the level of MDA, H2O2 and Cd uptake under Cd stress. Application of K and Si also enhanced the uptake of mineral nutrients including calcium (Ca), magnesium (Mg), manganese (Mn), sulfur (S) and K. The plants supplemented with K and Si exhibited a higher amount of total phenolics and flavonoids. The combined effect of Si and K was more pronounced regarding beneficial effects on gladiolus plants compared to individual effect of these elements under Cd stress. The current research reveals that Si and K may improve gladiolus growth by decreasing the oxidative stress and Cd uptake and by increasing the activity of antioxidant defense enzymes, the quantity of secondary metabolites and plant nutrition.

Role of Burkholderia cepacia CS8 in Cd-stress alleviation and phytoremediation by Catharanthus roseus

ABSTRACT The current study was performed to assess the effect of Burkholderia cepacia CS8 on the phytoremediation of cadmium (Cd) by Catharanthus roseus grown in Cd-contaminated soil. The plants cultivated in Cd amended soil showed reduced growth, dry mass, gas-exchange capacity, and chlorophyll contents. Furthermore, the plants exhibited elevated levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2) under Cd stress. The bacterized plants showed higher shoot length, root length; fresh and dry weight. The improved stress tolerance in inoculated plants was attributed to the reduced quantity of MDA and H2O2, enhanced synthesis of protein, proline, phenols, flavonoids, and improved activity of antioxidant enzymes including peroxidase, superoxide dismutase, ascorbate peroxidase, and catalase. Similarly, the 1-aminocyclopropane-1-carboxylate deaminase activity, phosphate solubilization, auxin, and siderophore production capability of B. cepacia CS8 improved growth and stress alleviation in treated plants. The bacterial inoculation enhanced the amount of water extractable Cd from soil. Furthermore, the inoculated plants showed higher bioconcentration factor and translocation factor. The current study exhibits that B. cepacia CS8 improves stress alleviation and phytoextraction potential of C. roseus plants growing under Cd stress.