Sally Noll

Antibiotic degradation during manure composting

On-farm manure management practices, such as composting, may provide a practical and economical option for reducing antibiotic concentrations in manure before land application, thereby minimizing the potential for environmental contamination. The objective of this study was to quantify degradation of chlortetracycline, monensin, sulfamethazine, and tylosin in spiked turkey (Meleagris gallopavo) litter during composting. Three manure composting treatments were evaluated: a control treatment (manure pile with no disturbance or adjustments after initial mixing), a managed compost pile (weekly mixing and moisture content adjustments), and vessel composting. Despite significant differences in temperature, mass, and nutrient losses between the composting treatments and the control, there was no difference in antibiotic degradation among the treatments. Chlortetracycline concentrations declined rapidly during composting, whereas monensin and tylosin concentrations declined gradually in all three treatments. There was no degradation of sulfamethazine in any of treatments. At the conclusion of the composting period (22-35 d), there was >99% reduction in chlortetracycline, whereas monensin and tylosin reduction ranged from 54 to 76% in all three treatments. Assuming first-order decay, the half-lives for chlortetracycline, monensin, and tylosin were 1, 17, and 19 d, respectively. These data suggest that managed compositing in a manure pile or in a vessel is not better than the control treatment in degrading certain antibiotics in manure. Therefore, low-level manure management, such as stockpiling, after an initial adjustment of water content may be a practical and economical option for livestock producers in reducing antibiotic levels in manure before land application.

Conversion of distiller's grain into fuel alcohol and a higher-value animal feed by dilute-acid pretreatment.

Over the past three decades ethanol production in the United States has increased more than 10-fold, to approx 2.9 billion gal/yr (mid-2003), with ethanol production expected to reach 5 billion gal/yr by 2005. The simultaneous coproduction of 7 million t/yr of distillers grain (DG) may potentially drive down the price of DG as a cattle feed supplement. The sale of residual DG for animal feed is an important part of corn dry-grind ethanol production economics; therefore, dry-grind ethanol producers are seeking ways to improve the quality of DG to increase market penetration and help stabilize prices. One possible improvement is to increase the protein content of DG by converting the residual starch and fiber into ethanol. We have developed methods for steam explosion, SO2, and dilute-sulfuric acid pretreatment of DG for evaluation as a feedstock for ethanol production. The highest soluble sugar yields (∼77% of available carbohydrate) were obtained by pretreatment of DG at 140°C for 20 min with 3.27 wt% H2SO4. Fermentation protocols for pretreated DG were developed at the bench scale and scaled to a working volume of 809 L for production of hydrolyzed distillers grain (HDG) for feeding trials. The pretreated DG was fermented with Saccharomyces cerevisiae D5A, with ethanol yields of 73% of theoretical from available glucans. The HDG was air-dried and used for turkey-feeding trials. The inclusion of HDG into turkey poult (as a model non-ruminant animal) diets at 5 and 10% levels, replacing corn and soybean meal, showed weight gains in the birds similar to controls, whereas 15 and 20% inclusion levels showed slight decreases (−6%) in weight gain. At the conclusion of the trial, no negative effects on internal organs or morphology, and no mortality among the poults, was found. The high protein levels (58–61%) available in HDG show promising economics for incorporation of this process into corn dry-grind ethanol plants.

Pathogenesis of Avian Pneumovirus Infection in Turkeys

Avian pneumovirus (APV) is the cause of a respiratory disease of turkeys characterized by coughing, ocular and nasal discharge, and swelling of the infraorbital sinuses. Sixty turkey poults were reared in isolation conditions. At 3 weeks of age, serum samples were collected and determined to be free of antibodies against APV, avian influenza, hemorrhagic enteritis, Newcastle disease, Mycoplasma gallisepticum, Mycoplasma synoviae, Mycoplasma meleagridis, Ornithobacterium rhinotracheale, and Bordetella avium. When the poults were 4 weeks old, they were inoculated with cell culture–propagated APV (APV/Minnesota/turkey/2a/97) via the conjunctival spaces and nostrils. After inoculation, four poults were euthanatized every 2 days for 14 days, and blood, swabs, and tissues were collected. Clinical signs consisting of nasal discharge, swelling of the infraorbital sinuses, and frothy ocular discharge were evident by 2 days postinoculation (PI) and persisted until day 12 PI. Mild inflammation of the mucosa of the nasal turbinates and infraorbital sinuses was present between days 2 and 10 PI. Mild inflammatory changes were seen in tracheas of poults euthanatized between days 4 and 10 PI. Antibody to APV was detected by day 7 PI. The virus was detected in tissue preparations and swabs of nasal turbinates and infraorbital sinuses by reverse transcription polymerase chain reaction, virus isolation, and immunohistochemical staining methods between days 2 and 10 PI. Virus was detected in tracheal tissue and swabs between days 2 and 6 PI using the same methods. In this experiment, turkey poults inoculated with tissue culture-propagated APV developed clinical signs similar to those seen in field cases associated with infection with this virus.

Avian pneumovirus infection in Minnesota turkeys: experimental reproduction of the disease.

Avian pneumovirus (APV) is an emerging viral respiratory disease agent of turkeys in Minnesota. Clinical signs of APV infection include open mouth breathing, ocular and nasal discharge, and swelling of infraorbital sinuses. The virus spreads rapidly among flocks of susceptible turkeys and is associated with increased mortality rates. A flock of 11-wk-old turkeys experienced a respiratory problem characterized by coughing, sneezing, swollen sinuses, and nasal discharge. The reverse transcriptase-polymerase chain reaction (RT-PCR) performed on tissues from the nasal turbinates and tracheal tissues was positive for avian pneumovirus. Turbinate tissue was inoculated into chicken embryo fibroblasts, and cytopathic effect was observed after five blind passages. In an attempt to reproduce the disease, 50 microl of this cell culture-propagated virus was instilled into each conjunctival space and nostril of 23-day-old turkey poults. The poults were sacrificed at 2-day intervals for 12 days, and serum, tissues, and tracheal and cloacal swabs were collected. Between days 2 and 10 after exposure, the poults developed ocular and nasal discharge and swollen sinuses. The virus was detected by RT-PCR and virus isolation from the nasal turbinates of poults sacrificed on days 4 and 6 postinoculation. Antibodies to APV were detected by enzyme-linked immunosorbent assay.

Succession of the turkey gastrointestinal bacterial microbiome related to weight gain

Because of concerns related to the use of antibiotics in animal agriculture, antibiotic-free alternatives are greatly needed to prevent disease and promote animal growth. One of the current challenges facing commercial turkey production in Minnesota is difficulty obtaining flock average weights typical of the industry standard, and this condition has been coined “Light Turkey Syndrome” or LTS. This condition has been identified in Minnesota turkey flocks for at least five years, and it has been observed that average flock body weights never approach their genetic potential. However, a single causative agent responsible for these weight reductions has not been identified despite numerous efforts to do so. The purpose of this study was to identify the bacterial community composition within the small intestines of heavy and light turkey flocks using 16S rRNA sequencing, and to identify possible correlations between microbiome and average flock weight. This study also sought to define the temporal succession of bacteria occurring in the turkey ileum. Based upon 2.7 million sequences across nine different turkey flocks, dominant operational taxonomic units (OTUs) were identified and compared between the flocks studied. OTUs that were associated with heavier weight flocks included those with similarity to Candidatus division Arthromitus and Clostridium bartlettii, while these flocks had decreased counts of several Lactobacillus species compared to lighter weight flocks. The core bacterial microbiome succession in commercial turkeys was also defined. Several defining markers of microbiome succession were identified, including the presence or abundance of Candidatus division Arthromitus, Lactobacillus aviarius, Lactobacillus ingluviei, Lactobacillus salivarius, and Clostridium bartlettii. Overall, the succession of the ileum bacterial microbiome in commercial turkeys proceeds in a predictable manner. Efforts to prevent disease and promote growth in the absence of antibiotics could involve target dominant bacteria identified in the turkey ileum that are associated with increased weight gain.

Effects of Bacterial Coinfection on the Pathogenesis of Avian Pneumovirus Infection in Turkeys

Abstract Four- and nine-week-old poults were inoculated with cell culture propagated avian pneumovirus (APV) into each conjunctival space and nostril, followed by inoculation 3 days later with Escherichia coli, Bordetella avium (BA), or Ornithobacterium rhinotracheale or a mixture of all three (EBO). Clinical signs were evaluated on days 3, 5, 7, 9, 11, and 14 postinoculation (PI) of APV. The poults were euthanatized on days 2, 4, 6, 10, and 14 PI, and blood and tissues were collected. The poults that received APV followed by EBO or BA alone developed more severe clinical signs related to nasal discharge and swelling of intraorbital sinuses than did poults inoculated with APV alone or bacteria alone. More severe pathologic changes were found in poults inoculated with APV+BA that extended to the air sacs and lungs, particularly in 9-wk-old poults. Bordetella avium was recovered from tracheas and lungs of birds that were inoculated with APV followed by EBO or BA alone. APV was detected by immunohistochemical staining in the upper respiratory tract longer in the groups of poults inoculated with APV and pathogenic bacteria than in those that received only APV, particularly when BA was involved. Viral antigen was also detected in the lungs of poults that were inoculated with APV followed by administration of EBO or BA alone. Loss of cilia on the epithelial surface of the upper respiratory tract was associated with BA infection and may enhance infection with APV, allowing deeper penetration of the virus into the respiratory tract.

Immunohistochemical detection of avian pneumovirus in formalin-fixed tissues

An immunohistochemical staining technique (IHC) was developed to detect avian pneumovirus (APV) antigen in formalin-fixed, paraffin-embedded tissue sections using streptavidin-biotin immunoperoxidase staining. Samples of nasal turbinates and infraorbital sinuses were collected from 4-week-old poults experimentally inoculated with APV and from older turkeys infected during naturally occurring outbreaks of avian pneumovirus. Tissue was fixed in 10% buffered neutral formalin, embedded in paraffin, sectioned and stained. Inflammatory changes were observed microscopically in the mucosa and submucosa of the nasal turbinates and infraorbital sinuses of both experimentally inoculated poults and naturally infected birds. Viral antigen was detected by IHC in the ciliated epithelial cells of nasal turbinates and infraorbital sinuses.

Ammonia and PM Emissions from a Tom Turkey Barn in Iowa

Considerable progress has been made toward collection of baseline data on air emissions from U.S. animal feeding operations. However, limited data exist in the literature regarding turkey air emissions. The project described in this paper continuously monitors ammonia (NH3) and particulate matter (PM) emissions from turkey production houses in Iowa (IA) and Minnesota (MN) for one year, with IA monitoring Hybrid tom turkeys and MN monitoring Hybrid hens. Mobile Air Emission Monitoring Units are used in the continuous monitoring. Data collection and analysis has been ongoing since May 2, 2007 for the IA site and October 9, 2007 for the MN site. Based on the one-year measurement at the IA site involving three flocks, daily NH3 emissions (g/d-bird) from the IA turkey house varied from 0.04 to 6.4 (mean of 1.9) for flock 1 (May-Aug), 0.2 to 3.4 (mean of 1.3) for flock 2 (Aug-Dec), and 0.16 to 3.8 (mean 1.4) for flock 3 (Dec-Apr). The PM10 emissions (g/d-bird) were 0.04 to 1.6 (mean of 0.58), 0.04 to 0.39 (mean of 0.2), and 0.04 to 0.82 (mean of 0.37) for flocks 1, 2, and 3, respectively; and the concomitant PM2.5 emissions (g/d-bird) were 0 to 0.11 (mean of 0.048), 0 to 0.05 (mean of 0.021), and 0 to 0.14 (mean of 0.053) for flocks 1, 2, and 3, respectively. Annual mean emissions from the tom turkeys (including downtime emission), expressed as grams of constituent per bird marketed, were 169 g NH3, 40 g PM10, and 4.3 g PM2.5 per bird marketed. Data collection and analysis at the MN site are ongoing.

Air Emissions from Tom and Hen Turkey Houses in the U.S. Midwest

Limited data exist in the literature regarding air emissions from U.S. turkey feeding operations. The project described in this article continuously monitored ammonia (NH3) and particulate matter (PM) emissions from turkey production houses in Iowa (IA) and Minnesota (MN) for 10 to 16 months, with IA monitoring Hybrid tom turkeys (35 to 143 d of age, average market body weight of 17.9 kg) for 16 months and MN monitoring Hybrid hens (35 to 84 d of age, average market body weight of 6.7 kg) for 10 months. Mobile air emission monitoring units (MAEMUs) were used in the continuous monitoring. Based on the approximately one-year measurement, each involving three flocks of birds, daily NH3, PM10, and PM2.5 concentrations (mean ±SD) in the tom turkey barn were 8.6 ±10.0 ppm, 1104 ±719 µg m-3, and 143 (±124) µg m-3, respectively. Daily NH3 and PM10 concentrations (mean ±SD) in the hen turkey barn were 7.3 ±7.9 ppm and 301 ±160 µg m-3, respectively. Daily NH3 concentrations during downtime (mean ±SD) were 38.4 ±20.5 and 20.0 ±16.3 ppm in the tom and hen barns, respectively. The cumulative NH3 emissions (mean ±SE) were 141 ±13.1 and 1.8 ±0.9 g bird-1 for the tom turkeys during 108 d growout and 13 d downtime, respectively, and 52 ±2.1 and 28.2 ±2.5 g bird-1 for the hen turkeys during 49 d growout and 32 d downtime, respectively (the extended downtime for the hen house was to ensure monitoring of one flock per season). The cumulative PM10 emission (mean ±SE) was 28.2 ±3.3 g bird-1 for the tom turkeys during 108 d growout and 4.6 ±2.2 and 0.3 ±0.06 g bird-1 for the hen turkeys during 49 d growout and 32 d downtime, respectively. Downtime in the hen house was of greater duration than would be typically observed (32 d vs. 7 d to 14 d typical). The cumulative PM2.5 emission (mean ±SE) was 3.6 ±0.7 g bird-1 for the tom turkeys during 108 d growout (not monitored for the hen turkeys). Because farm operations will vary in flock number, growout days, and downtime; annual emissions can be calculated from the cumulative emissions and downtime emissions per bird from the data provided. Air emissions data from this study, presented in both daily emission and cumulative per-bird-marketed emission, contribute to the improved U.S. national air emissions inventory for animal feeding operations.

Market Turkey Performance Affected by Floor Type and Brooding Method

Two experiments with 320 Large White male turkeys (Nicholas strain) investigated the effect of partial replacement of litter with different types of slotted flooring (fiberglass, concrete, or wood in Experiment 1 and poly-vinyl chloride pipe, concrete, or wood in Experiment 2) and the effect of brooding method (cage or floor) on later growing performance. Growth, mortality, feed efficiency, and foot pad dermatitis were not affected by partial replacement of the litter floor area with slotted flooring. Excreta buildup due to narrow slot width was a problem in Experiment 1. Turkeys reared on deep litter had greater breast blister scores than turkeys reared on the wider-slotted flooring in Experiment 2. Slotted flooring reduced litter moisture in both experiments. Cage-brooded turkeys in Experiment 1 were heavier than floor-brooded turkeys at three weeks of age, had greater mortality, and in Experiment 2 had more breast blisters.